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  1. Article ; Online: Reverse Genetics of Dengue Virus.

    Silva Júnior, José Valter Joaquim / da Silva, Andréa Nazaré Monteiro Rangel / da Silva Santos, Jefferson José / Gil, Laura Helena Vega Gonzales

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2733, Page(s) 231–248

    Abstract: Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV ... ...

    Abstract Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV biology, which have been facilitated by the development of different molecular strategies for engineering viral genomes, such as reverse genetics approaches. Reverse genetic systems are a powerful tool for investigating virus-host interaction, for vaccine development, and for high-throughput screening of antiviral compounds. However, stable manipulation of DENV genomes is a major molecular challenge, especially when using conventional cloning systems. To circumvent this issue, we describe a simple and efficient yeast-based reverse genetics system to recover infectious DENV clones.
    MeSH term(s) Humans ; Dengue Virus/genetics ; Reverse Genetics ; High-Throughput Screening Assays ; Genome, Viral ; Dengue/genetics ; Virus Replication/genetics
    Language English
    Publishing date 2023-12-08
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3533-9_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Detection of Human polyomavirus 2 (HPyV2) in oyster samples in northern Brazil.

    Abreu, Isabella Nogueira / Cortinhas, Jacqueline Monteiro / Dos Santos, Mike Barbosa / Queiroz, Maria Alice Freitas / da Silva, Andréa Nazaré Monteiro Rangel / Cayres-Vallinoto, Izaura Maria Vieira / Vallinoto, Antonio Carlos Rosário

    Virology journal

    2020  Volume 17, Issue 1, Page(s) 85

    Abstract: Background: Human polyomavirus 2 (HPyV2 or JCPyV) is persistent in the environment due to its excretion in urine and feces; it is detected in samples of wastewater, surface water and drinking water. A lack of basic sanitation and sewage collection ... ...

    Abstract Background: Human polyomavirus 2 (HPyV2 or JCPyV) is persistent in the environment due to its excretion in urine and feces; it is detected in samples of wastewater, surface water and drinking water. A lack of basic sanitation and sewage collection results in the presence of this virus in food, especially in oysters, since they are bioaccumulators and are consumed in their natural form, thus posing a risk to human health.
    Methods: This study investigated the frequency of HPyV2 in samples of oysters marketed in northeastern Pará State, Brazil, and optimized a real-time PCR (qPCR) protocol for the detection of an endogenous oyster control. A total of 217 oysters in 22 pools from five municipalities in the state of Pará were analyzed. Samples underwent dissection and total maceration of oyster tissue using a viral concentration technique, followed by DNA extraction with phenol-chloroform and amplification of the VP1 region for molecular detection via qPCR.
    Results: HPyV2 was detected in 18.2% (4/22) of the pooled samples, with frequencies of 25, 20, 20 and 16% in the municipalities of Salinópolis, Augusto Corrêa, São Caetano de Odivelas and Curuçá, respectively. Notably, the sample pool from the municipality of Bragança did not have detectable HPyV2 and this was the only sampled location with a water treatment station. In this study, Crassostrea genus-specific primers (AFL52 ribosomal RNA gene) of oyster were developed for use as an endogenous control in the qPCR analysis, which will be useful for future studies.
    Conclusions: The detection of HPyV2 in oyster samples commercialized in the state of Pará shows the circulation of this virus in the studied municipalities. Thus, it is necessary to implement measures for improving sewage collection and basic sanitation to avoid contamination of water and food with HPyV2.
    MeSH term(s) Animals ; Brazil ; Environmental Monitoring ; Humans ; Ostreidae/virology ; Polyomavirus/genetics ; Polyomavirus/isolation & purification ; Sewage/virology ; Water Microbiology ; Water Purification
    Chemical Substances Sewage
    Language English
    Publishing date 2020-06-26
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1743-422X
    ISSN (online) 1743-422X
    DOI 10.1186/s12985-020-01360-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: STING and cGAS gene expressions were downregulated among HIV-1-infected persons after antiretroviral therapy.

    de Lima, Lorena Leticia Peixoto / de Oliveira, Allysson Quintino Tenório / Moura, Tuane Carolina Ferreira / da Silva Graça Amoras, Ednelza / Lima, Sandra Souza / da Silva, Andrea Nazaré Monteiro Rangel / Queiroz, Maria Alice Freitas / Cayres-Vallinoto, Izaura Maria Vieira / Ishak, Ricardo / Vallinoto, Antonio Carlos Rosário

    Virology journal

    2021  Volume 18, Issue 1, Page(s) 78

    Abstract: Background: The HIV-1 epidemic is still considered a global public health problem, but great advances have been made in fighting it by antiretroviral therapy (ART). ART has a considerable impact on viral replication and host immunity. The production of ... ...

    Abstract Background: The HIV-1 epidemic is still considered a global public health problem, but great advances have been made in fighting it by antiretroviral therapy (ART). ART has a considerable impact on viral replication and host immunity. The production of type I interferon (IFN) is key to the innate immune response to viral infections. The STING and cGAS proteins have proven roles in the antiviral cascade. The present study aimed to evaluate the impact of ART on innate immunity, which was represented by STING and cGAS gene expression and plasma IFN-α level.
    Methods: This cohort study evaluated a group of 33 individuals who were initially naïve to therapy and who were treated at a reference center and reassessed 12 months after starting ART. Gene expression levels and viral load were evaluated by real-time PCR, CD4
    Results: From before to after ART, the CD4
    Conclusions: ART provided immune recovery and viral suppression to the studied group and indirectly downregulated the STING and cGAS genes. In contrast, ART did not influence IFN-α. The expression of STING and cGAS was not correlated with the plasma level of IFN-α, which suggests that there is another pathway regulating this cytokine in addition to the STING-cGAS pathway.
    MeSH term(s) Anti-HIV Agents/therapeutic use ; Cohort Studies ; Gene Expression ; HIV Infections/drug therapy ; HIV-1/genetics ; HIV-1/metabolism ; Humans ; Interferon-alpha/blood ; Membrane Proteins/genetics ; Nucleotidyltransferases/genetics ; Signal Transduction
    Chemical Substances Anti-HIV Agents ; Interferon-alpha ; Membrane Proteins ; STING1 protein, human ; Nucleotidyltransferases (EC 2.7.7.-) ; cGAS protein, human (EC 2.7.7.-)
    Language English
    Publishing date 2021-04-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2160640-7
    ISSN 1743-422X ; 1743-422X
    ISSN (online) 1743-422X
    ISSN 1743-422X
    DOI 10.1186/s12985-021-01548-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Antibody Response to the SARS-CoV-2 Spike and Nucleocapsid Proteins in Patients with Different COVID-19 Clinical Profiles.

    Soares, Sinei Ramos / da Silva Torres, Maria Karoliny / Lima, Sandra Souza / de Sarges, Kevin Matheus Lima / Santos, Erika Ferreira Dos / de Brito, Mioni Thieli Figueiredo Magalhães / da Silva, Andréa Luciana Soares / de Meira Leite, Mauro / da Costa, Flávia Póvoa / Cantanhede, Marcos Henrique Damasceno / da Silva, Rosilene / de Oliveira Lameira Veríssimo, Adriana / Vallinoto, Izaura Maria Vieira Cayres / Feitosa, Rosimar Neris Martins / Quaresma, Juarez Antônio Simões / Chaves, Tânia do Socorro Souza / Viana, Giselle Maria Rachid / Falcão, Luiz Fábio Magno / Santos, Eduardo José Melo Dos /
    Vallinoto, Antonio Carlos Rosário / da Silva, Andréa Nazaré Monteiro Rangel

    Viruses

    2023  Volume 15, Issue 4

    Abstract: The first case of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in Brazil was diagnosed on February 26, 2020. Due to the important epidemiological impact of COVID-19, the present study aimed ... ...

    Abstract The first case of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in Brazil was diagnosed on February 26, 2020. Due to the important epidemiological impact of COVID-19, the present study aimed to analyze the specificity of IgG antibody responses to the S1, S2 and N proteins of SARS-CoV-2 in different COVID-19 clinical profiles. This study enrolled 136 individuals who were diagnosed with or without COVID-19 based on clinical findings and laboratory results and classified as asymptomatic or as having mild, moderate or severe disease. Data collection was performed through a semistructured questionnaire to obtain demographic information and main clinical manifestations. IgG antibody responses to the S1 and S2 subunits of the spike (S) protein and the nucleocapsid (N) protein were evaluated using an enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's instructions. The results showed that among the participants, 87.5% (119/136) exhibited IgG responses to the S1 subunit and 88.25% (120/136) to N. Conversely, only 14.44% of the subjects (21/136) displayed S2 subunit responses. When analyzing the IgG antibody response while considering the different proteins of the virus, patients with severe disease had significantly higher antibody responses to N and S1 than asymptomatic individuals (
    MeSH term(s) Humans ; Antibodies, Viral ; Antibody Formation ; COVID-19 ; Immunoglobulin G ; Nucleocapsid Proteins ; Post-Acute COVID-19 Syndrome ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
    Chemical Substances Antibodies, Viral ; Immunoglobulin G ; Nucleocapsid Proteins ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2023-03-31
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15040898
    Database MEDical Literature Analysis and Retrieval System OnLINE

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