Article ; Online: Reverse Genetics of Dengue Virus.
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2733, Page(s) 231–248
Abstract: Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV ... ...
| Abstract | Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV biology, which have been facilitated by the development of different molecular strategies for engineering viral genomes, such as reverse genetics approaches. Reverse genetic systems are a powerful tool for investigating virus-host interaction, for vaccine development, and for high-throughput screening of antiviral compounds. However, stable manipulation of DENV genomes is a major molecular challenge, especially when using conventional cloning systems. To circumvent this issue, we describe a simple and efficient yeast-based reverse genetics system to recover infectious DENV clones. |
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| MeSH term(s) | Humans ; Dengue Virus/genetics ; Reverse Genetics ; High-Throughput Screening Assays ; Genome, Viral ; Dengue/genetics ; Virus Replication/genetics |
| Language | English |
| Publishing date | 2023-12-08 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-0716-3533-9_14 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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