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  1. Article ; Online: Report of Salmonella Saintpaul from chicken carcass co-harbouring blaCTX-M2 and mcr-9.1.

    de Almeida Figueira, Arthur / Salles Dias, Thomas / Alves Costa, Gisllany / de Souza, Giovanna Silva / da Costa Abreu, Dayse Lima / Dos Santos Medeiros, Luciana / de Almeida Pereira, Virginia Léo

    Journal of global antimicrobial resistance

    2024  Volume 37, Page(s) 22–23

    Language English
    Publishing date 2024-02-27
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 2710046-7
    ISSN 2213-7173 ; 2213-7173
    ISSN (online) 2213-7173
    ISSN 2213-7173
    DOI 10.1016/j.jgar.2024.02.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Correction to: SVR-flaA typing of erythromycin- and ciprofloxacin-resistant Campylobacter jejuni strains isolated from poultry slaughterhouses in southern Brazil.

    Dias, Thomas Salles / de Almeida Figueira, Arthur / Costa, Gisllany Alves / da Cunha, Nathalie Costa / Rossi, Daise Aparecida / de Melo, Roberta Torres / de Almeida Pereira, Virginia Léo / de Aquino, Maria Helena Cosendey

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2023  Volume 54, Issue 2, Page(s) 1323

    Language English
    Publishing date 2023-04-27
    Publishing country Brazil
    Document type Published Erratum
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1517-8382
    ISSN (online) 1678-4405
    ISSN 1517-8382
    DOI 10.1007/s42770-023-00983-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: SVR-flaA typing of erythromycin- and ciprofloxacin-resistant Campylobacter jejuni strains isolated from poultry slaughterhouses in southern Brazil.

    Dias, Thomas Salles / de Almeida Figueira, Arthur / Costa, Gisllany Alves / da Cunha, Nathalie Costa / Rossi, Daise Aparecida / de Melo, Roberta Torres / de Almeida Pereira, Virginia Léo / de Aquino, Maria Helena Cosendey

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2023  Volume 54, Issue 2, Page(s) 1065–1073

    Abstract: The emergence of fluoroquinolone and macrolide resistance in C. jejuni, a recognized zoonotic pathogen, has increased worldwide. This study aimed to investigate phenotypic resistance to ciprofloxacin and erythromycin, the molecular mechanisms involved, ... ...

    Abstract The emergence of fluoroquinolone and macrolide resistance in C. jejuni, a recognized zoonotic pathogen, has increased worldwide. This study aimed to investigate phenotypic resistance to ciprofloxacin and erythromycin, the molecular mechanisms involved, and the strain of C. jejuni isolated from broiler carcasses. Eighty C. jejuni isolates from broiler carcasses in southern Brazil were investigated for their susceptibility to ciprofloxacin and erythromycin at minimal inhibitory concentrations. Mismatch amplification mutation assay-polymerase chain reaction (MAMA-PCR) was performed to detect substitutions of Thr-86-Ile, A2074C, and A2075G of domain V in the 23S rRNA. The presence of ermB gene and CmeABC operon were investigated by PCR. DNA sequencing was used to detect substitutions in the L4 and L22 proteins of the erythromycin-resistant strains. The Short Variable Region (SVR) of flaA was used to type all the strains resistant to both antimicrobials. Ciprofloxacin and erythromycin resistance were detected in 81.25% and 30.00% of the strains, respectively, and minimal inhibitory concentration values ranged from ≤ 0.125 to 64 µg/mL for ciprofloxacin and 0.5 to > 128 µg/mL for erythromycin. The Thr-86-Ile mutation in gyrA was observed in 100% of the ciprofloxacin-resistant strains. Mutations in both the A2074C and A2075G positions of 23S rRNA were observed in 62.5% of the erythromycin-resistant strains, while 37.5% had only the mutation A2075G. None of the strains harbored CmeABC operon, and ermB was not detected. Using DNA sequencing, the amino acid substitution T177S was detected in L4, and substitutions I65V, A103V, and S109A were detected in L22. Twelve flaA-SVR alleles were identified among the strains, with the most common SVR-flaA allele, type 287, covering 31.03% of ciprofloxacin- and erythromycin-resistant isolates. The present study revealed a high incidence and high levels of resistance to ciprofloxacin and erythromycin, as well as broad molecular diversity in C. jejuni isolates from broiler carcasses.
    MeSH term(s) Animals ; Ciprofloxacin/pharmacology ; Erythromycin/pharmacology ; Anti-Bacterial Agents/pharmacology ; Campylobacter jejuni/genetics ; Poultry ; Abattoirs ; Brazil ; RNA, Ribosomal, 23S/genetics ; Campylobacter Infections/microbiology ; Drug Resistance, Bacterial/genetics ; Macrolides/pharmacology ; Chickens/microbiology ; Microbial Sensitivity Tests
    Chemical Substances Ciprofloxacin (5E8K9I0O4U) ; Erythromycin (63937KV33D) ; Anti-Bacterial Agents ; RNA, Ribosomal, 23S ; Macrolides
    Language English
    Publishing date 2023-04-13
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1517-8382
    ISSN (online) 1678-4405
    ISSN 1517-8382
    DOI 10.1007/s42770-023-00969-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Molecular markers associated with antimicrobial resistance and genotypes of Campylobacter jejuni and Campylobacter coli isolated from broiler and swine flocks in southeast Brazil

    Dias, Thomas Salles / Costa, Gisllany Alves / de Almeida Figueira, Arthur / dos Santos Machado, Leandro / da Cunha, Nathalie Costa / do Nascimento, Elmiro Rosendo / de Almeida Pereira, Virginia Léo / de Aquino, Maria Helena Cosendey

    Comparative immunology, microbiology, and infectious diseases. 2022 Sept., v. 88

    2022  

    Abstract: This study aimed to identify molecular markers associated with antimicrobial resistance and genotype isolates of Campylobacter spp. from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C. coli (n = 35) strains were ... ...

    Abstract This study aimed to identify molecular markers associated with antimicrobial resistance and genotype isolates of Campylobacter spp. from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C. coli (n = 35) strains were screened for the antimicrobial genetic markers C257T in gyrA, A2074C and A2075G in 23S rRNA, CmeABC, ermB, tetO and blaOXA61 by PCR. Fifteen strains had SVR-flaA and porA genes sequenced to evaluate their genetic diversity. Among C. jejuni strains 62.96% had C257T mutation and only one strain had A2075G mutation. CmeA, cmeB, cmeC, tetO and blaOXA61 were detected respectively in 92.59%, 100%, 100%, 85.19%, 85.19% of the strains. All C. coli had C257T mutation; 48.75% had A2075G and cmeA, cmeB, cmeC, tetO, blaOXA61 were detected in 8.57%, 94.29%, 91.43%, 91.43%, 80%, respectively. Twelve porA and SVR-flaA alleles were detected, with a Simpson index of diversity value of 0.962.
    Keywords Campylobacter coli ; Campylobacter jejuni ; antibiotic resistance ; genetic variation ; genotype ; immunology ; microbiology ; mutation ; swine ; Brazil
    Language English
    Dates of publication 2022-09
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 436522-7
    ISSN 1878-1667 ; 0147-9571
    ISSN (online) 1878-1667
    ISSN 0147-9571
    DOI 10.1016/j.cimid.2022.101866
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1443: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Molecular markers associated with antimicrobial resistance and genotypes of Campylobacter jejuni and Campylobacter coli isolated from broiler and swine flocks in southeast Brazil.

    Dias, Thomas Salles / Costa, Gisllany Alves / de Almeida Figueira, Arthur / Dos Santos Machado, Leandro / da Cunha, Nathalie Costa / do Nascimento, Elmiro Rosendo / de Almeida Pereira, Virginia Léo / de Aquino, Maria Helena Cosendey

    Comparative immunology, microbiology and infectious diseases

    2022  Volume 88, Page(s) 101866

    Abstract: This study aimed to identify molecular markers associated with antimicrobial resistance and genotype isolates of Campylobacter spp. from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C. coli (n = 35) strains were ... ...

    Abstract This study aimed to identify molecular markers associated with antimicrobial resistance and genotype isolates of Campylobacter spp. from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C. coli (n = 35) strains were screened for the antimicrobial genetic markers C257T in gyrA, A2074C and A2075G in 23S rRNA, CmeABC, ermB, tetO and blaOXA61 by PCR. Fifteen strains had SVR-flaA and porA genes sequenced to evaluate their genetic diversity. Among C. jejuni strains 62.96% had C257T mutation and only one strain had A2075G mutation. CmeA, cmeB, cmeC, tetO and blaOXA61 were detected respectively in 92.59%, 100%, 100%, 85.19%, 85.19% of the strains. All C. coli had C257T mutation; 48.75% had A2075G and cmeA, cmeB, cmeC, tetO, blaOXA61 were detected in 8.57%, 94.29%, 91.43%, 91.43%, 80%, respectively. Twelve porA and SVR-flaA alleles were detected, with a Simpson index of diversity value of 0.962.
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents/pharmacology ; Brazil/epidemiology ; CME-Carbodiimide ; Campylobacter Infections/epidemiology ; Campylobacter Infections/veterinary ; Campylobacter coli/genetics ; Campylobacter jejuni ; Chickens ; Drug Resistance, Bacterial/genetics ; Genotype ; Swine ; Swine Diseases
    Chemical Substances Anti-Bacterial Agents ; Anti-Infective Agents ; CME-Carbodiimide (2491-17-0)
    Language English
    Publishing date 2022-08-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 436522-7
    ISSN 1878-1667 ; 0147-9571
    ISSN (online) 1878-1667
    ISSN 0147-9571
    DOI 10.1016/j.cimid.2022.101866
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1443: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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