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  1. Article ; Online: Advancing mechanobiology by performing whole-cell patch clamp recording on mechanosensitive cells subjected simultaneously to dynamic stretch events.

    de Coulon, Etienne / Dellenbach, Christian / Rohr, Stephan

    iScience

    2021  Volume 24, Issue 2, Page(s) 102041

    Abstract: A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing ... ...

    Abstract A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing simultaneously
    Language English
    Publishing date 2021-01-07
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2021.102041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation.

    Desbiolles, Benoît X E / de Coulon, Etienne / Maïno, Nicolas / Bertsch, Arnaud / Rohr, Stephan / Renaud, Philippe

    Microsystems & nanoengineering

    2020  Volume 6, Page(s) 67

    Abstract: Volcano-shaped microelectrodes (nanovolcanoes) functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access. These nanovolcanoes ... ...

    Abstract Volcano-shaped microelectrodes (nanovolcanoes) functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access. These nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion mechanism. In this study, we investigated whether the use of electroporation improves the performance of nanovolcano arrays in terms of action potential amplitudes, recording durations, and yield. Experiments with neonatal rat cardiomyocyte monolayers grown on nanovolcano arrays demonstrated that electroporation pulses with characteristics derived from analytical models increased the efficiency of nanovolcano recordings, as they enabled multiple on-demand registration of intracellular action potentials with amplitudes as high as 62 mV and parallel recordings in up to ~76% of the available channels. The performance of nanovolcanoes showed no dependence on the presence of functionalized nanopatterns, indicating that the tip geometry itself is instrumental for establishing a tight seal at the cell-electrode interface, which ultimately determines the quality of recordings. Importantly, the use of electroporation permitted the recording of attenuated cardiomyocyte action potentials during consecutive days at identical sites, indicating that nanovolcano recordings are nondestructive and permit long-term on-demand recordings from excitable cardiac tissues. Apart from demonstrating that less complex manufacturing processes can be used for next-generation nanovolcano arrays, the finding that the devices are suitable for performing on-demand recordings of electrical activity from multiple sites of excitable cardiac tissues over extended periods of time opens the possibility of using the devices not only in basic research but also in the context of comprehensive drug testing.
    Language English
    Publishing date 2020-08-24
    Publishing country England
    Document type Journal Article
    ISSN 2055-7434
    ISSN (online) 2055-7434
    DOI 10.1038/s41378-020-0178-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: High-speed mechano-active multielectrode array for investigating rapid stretch effects on cardiac tissue.

    Imboden, Matthias / de Coulon, Etienne / Poulin, Alexandre / Dellenbach, Christian / Rosset, Samuel / Shea, Herbert / Rohr, Stephan

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 834

    Abstract: Systematic investigations of the effects of mechano-electric coupling (MEC) on cellular cardiac electrophysiology lack experimental systems suitable to subject tissues to in-vivo like strain patterns while simultaneously reporting changes in electrical ... ...

    Abstract Systematic investigations of the effects of mechano-electric coupling (MEC) on cellular cardiac electrophysiology lack experimental systems suitable to subject tissues to in-vivo like strain patterns while simultaneously reporting changes in electrical activation. Here, we describe a self-contained motor-less device (mechano-active multielectrode-array, MaMEA) that permits the assessment of impulse conduction along bioengineered strands of cardiac tissue in response to dynamic strain cycles. The device is based on polydimethylsiloxane (PDMS) cell culture substrates patterned with dielectric actuators (DEAs) and compliant gold ion-implanted extracellular electrodes. The DEAs induce uniaxial stretch and compression in defined regions of the PDMS substrate at selectable amplitudes and with rates up to 18 s
    MeSH term(s) Animals ; Cardiac Electrophysiology/instrumentation ; Cardiac Electrophysiology/methods ; Cells, Cultured ; Dimethylpolysiloxanes ; Electric Stimulation/instrumentation ; Electric Stimulation/methods ; Electrodes, Implanted ; Equipment Design ; Myocytes, Cardiac/physiology ; Rats, Wistar
    Chemical Substances Dimethylpolysiloxanes ; baysilon (63148-62-9)
    Language English
    Publishing date 2019-02-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-08757-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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