Article ; Online: Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein: Targets for COVID-19 symptomatology and disease control.
European journal of immunology
2023 Volume 53, Issue 4, Page(s) e2250206
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) still poses a challenge for biomedicine and public health. To advance the development of effective diagnostic, prognostic, and preventive interventions, our study focused on high-throughput ... ...
| Abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) still poses a challenge for biomedicine and public health. To advance the development of effective diagnostic, prognostic, and preventive interventions, our study focused on high-throughput antibody binding epitope mapping of the SARS-CoV-2 spike RBD protein by IgA, IgM and IgG antibodies in saliva and sera of different cohorts from healthy uninfected individuals to SARS-CoV-2-infected unvaccinated and vaccinated asymptomatic, recovered, nonsevere, and severe patients. Identified candidate diagnostic (455-LFRKSNLKPFERD-467), prognostic (395-VYADSFVIRGDEV-407-C-KLH, 332-ITNLCPFGEV-342-C-KLH, 352-AWNRKRI-358-C-KLH, 524-VCGPKKSTNLVKN-536-KLH), and protective (MKLLE-487-NCYFPLQSYGFQPTNGVG-504-GGGGS-446-GGNYNYLYRLFRKSNLKPFERD-467) epitopes were validated with sera from prevaccine and postvaccine cohorts. The results identified neutralizing epitopes and support that antibody recognition of linear B-cell epitopes in RBD protein is associated with antibody isotype and disease symptomatology. The findings in asymptomatic individuals suggest a role for anti-RBD antibodies in the protective response against SARS-CoV-2. The possibility of translating results into diagnostic interventions for the early diagnosis of asymptomatic individuals and prognosis of disease severity provides new tools for COVID-19 surveillance and evaluation of risks in hospitalized patients. These results, together with other approaches, may contribute to the development of new vaccines for the control of COVID-19 and other coronavirus-related diseases using a quantum vaccinomics approach through the combination of protective epitopes. |
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| MeSH term(s) | Humans ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19/diagnosis ; Epitope Mapping ; Epitopes, B-Lymphocyte ; SARS-CoV-2 | ||||||||||
| Chemical Substances | Antibodies, Neutralizing ; Antibodies, Viral ; Epitopes, B-Lymphocyte ; spike protein, SARS-CoV-2 | ||||||||||
| Language | English | ||||||||||
| Publishing date | 2023-02-01 | ||||||||||
| Publishing country | Germany | ||||||||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | ||||||||||
| ZDB-ID | 120108-6 | ||||||||||
| ISSN | 1521-4141 ; 0014-2980 | ||||||||||
| ISSN (online) | 1521-4141 | ||||||||||
| ISSN | 0014-2980 | ||||||||||
| DOI | 10.1002/eji.202250206 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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