LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 5 of total 5

Search options

  1. Article: Ghrelin misbalance affects mice embryo implantation and pregnancy success by uterine immune dysregulation and nitrosative stress.

    Luque, Eugenia Mercedes / Díaz-Luján, Cintia María / Paira, Daniela Andrea / de Loredo, Nicolás / Torres, Pedro Javier / Cantarelli, Verónica Inés / Fretes, Ricardo / Motrich, Rubén Darío / Martini, Ana Carolina

    Frontiers in endocrinology

    2023  Volume 14, Page(s) 1288779

    Abstract: Introduction: In a previous study we found that ghrelin (Ghrl) misbalance during the peri-implantation period significantly impaired fetus development. In this study we aimed to evaluate the putative mechanisms underlying these effects, including embryo ...

    Abstract Introduction: In a previous study we found that ghrelin (Ghrl) misbalance during the peri-implantation period significantly impaired fetus development. In this study we aimed to evaluate the putative mechanisms underlying these effects, including embryo implantation success, uterine nitric oxide synthase (NOS) activity, nitric oxide synthesis and the inflammatory/immune uterine profile.
    Methods: Ghrelin misbalance was induced by injecting 4nmol/animal/day of Ghrl (hyperghrelinemia) or 6nmol/animal/day of a Ghrl antagonist (Ant: (D-Lys3)GHRP-6) from day 3 to 8 of pregnancy. Control animals (C) were injected with de vehicle. Females were euthanized at pregnancy day 8 and their uteri excised in order to evaluate: the percentage of reabsorbed embryos (microscopically), eNOS, iNOS and nytrotirosine expression (by immunohistochemistry), nitrite synthesis (by Griess technique), VEGF, IL-10, IL-17, IL-6, MMP9 and GM-CSF expression (by qPCR) and leukocyte infiltration by flow cytometry (evaluating T cells, NK cells, granulocytes, dendritic cells and macrophages).
    Results: Ant-treatment significantly increased the percentage of reabsorbed embryos and the uterine expression of eNOS, iNOS and nytrotirosine. (D-Lys3)GHRP-6-treatment increased also the expression of the inflammatory cytokines IL-6, IL-17 and MMP9, and decreased that of IL-10 (anti-inflammatory). Moreover, Ant-treatment increased also the NK cells population and that of CD11b
    Conclusion: Ghrl misbalance during the peri-implantation period induces pro-inflammatory changes and nitrosative stress in the gravid uterus, impairing significantly embryo implantation and/or development.
    MeSH term(s) Female ; Pregnancy ; Mice ; Animals ; Interleukin-10 ; Interleukin-17 ; Matrix Metalloproteinase 9 ; Ghrelin/pharmacology ; Nitrosative Stress ; Interleukin-6 ; Embryo Implantation ; Uterus
    Chemical Substances Interleukin-10 (130068-27-8) ; Interleukin-17 ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Ghrelin ; Interleukin-6
    Language English
    Publishing date 2023-12-01
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1288779
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Evolution of Risk Behaviors, Sexually Transmitted Infections and PrEP Care Continuum in a Hospital-Based PrEP Program in Barcelona, Spain: A Descriptive Study of the First 2 Years' Experience.

    Ugarte, Ainoa / de la Mora, Lorena / García, David / Martínez-Rebollar, María / de Lazzari, Elisa / Torres, Berta / Inciarte, Alexy / Ambrosioni, Juan / Chivite, Iván / Solbes, Estela / de Loredo, Nicolás / Del Carlo, Guillermo Federico / González-Cordón, Ana / Blanco, José Luis / Martínez, Esteban / Mallolas, Josep / Laguno, Montserrat

    Infectious diseases and therapy

    2022  Volume 12, Issue 2, Page(s) 425–442

    Abstract: Introduction: Pre-exposure prophylaxis (PrEP) is effective for HIV prevention, but the PrEP care continuum also involves improving PrEP awareness, uptake, adherence, and retention in care. Users' awareness is often compromised because of vulnerability ... ...

    Abstract Introduction: Pre-exposure prophylaxis (PrEP) is effective for HIV prevention, but the PrEP care continuum also involves improving PrEP awareness, uptake, adherence, and retention in care. Users' awareness is often compromised because of vulnerability factors and risk behaviors, such as chemsex practice or specific substance use, which could lead to risk compensation. Correct adherence and retention in care are essential to achieve the full effectiveness of PrEP. This study describes changes in users' risk behaviors and sexually transmitted infections (STIs), as well also PrEP care continuum details.
    Methods: This was a descriptive single-center retrospective study including adults at high HIV risk screened between November 2019 and June 2021 in the PrEP program of our hospital. Demographic, behavioral, STI, adherence, and retention in care variables were assessed. Data were collected from medical records and self-report questionnaires.
    Results: A total of 295 people were included, 94% men and 5% transgender women, with a mean age of 34 years (SD 10) and 10% sex workers. At baseline, 55% disclosed chemsex practice and 3% slamming. During follow-up, condom use for anal intercourse decreased from 41% to 13% (p ≤ 0.0001) and one HIV infection was detected; other risk behaviors and STIs remained stable. Chemsex, group sex, fluid exchange, and condomless anal intercourse were related to STI risk. Adherence was correct in 80% of users, and retention in care was 57%. Discontinuations and loss to follow-up were high, mainly affecting transgender women, sex workers, and people practicing fisting.
    Conclusion: PrEP program implementation in our hospital was adequate, since it allowed, in a population at high HIV risk, overall users' risk behaviors and STIs to remain stable, with only one HIV diagnosis during the follow-up. We should target specific strategies to improve adherence and retention in care, as vulnerable subgroups at higher risk of loss to follow-up are identified.
    Language English
    Publishing date 2022-12-15
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2701611-0
    ISSN 2193-6382 ; 2193-8229
    ISSN (online) 2193-6382
    ISSN 2193-8229
    DOI 10.1007/s40121-022-00733-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Chemsex Practices in PrEP: Beyond Addiction and Risk Toward a Healthy Sex Life-Baseline Experiences from a Hospital-Based PrEP Program in Barcelona, Spain.

    De La Mora, Lorena / Ugarte, Ainoa / Martínez-Rebollar, Maria / De Lazzari, Elisa / García-Hernández, David / Font, Guillermo / De Loredo, Nicolás / Solbes, Estela / Miquel, Laia / Blanch, Jordi / Torres, Berta / Riera, Josep / Chivite, Iván / Ambrosioni, Juan / Inciarte, Alexy / González-Cordón, Ana / Martínez, Esteban / Blanco, José Luis / Mallolas, Josep /
    Laguno, Montserrat

    AIDS and behavior

    2022  Volume 26, Issue 12, Page(s) 4055–4062

    Abstract: Pre-exposure prophylaxis (PrEP) is a biomedical intervention that has demonstrated efficacy in HIV prevention in individuals at high-risk, among them chemsex users. Out of 190 PrEP users followed at Hospital Clinic of Barcelona until October 2020, 89% ... ...

    Abstract Pre-exposure prophylaxis (PrEP) is a biomedical intervention that has demonstrated efficacy in HIV prevention in individuals at high-risk, among them chemsex users. Out of 190 PrEP users followed at Hospital Clinic of Barcelona until October 2020, 89% reported drug use, and 63% disclosed that they had engaged in chemsex practices, initiated in 64% of cases within the past year. Twenty-one percent used 3 or more drugs simultaneously, being GHB/GBL, nitrites, sildenafil, and methamphetamine the most prevalent combination. Eight percent reported slamming. Forty-one percent described having had negative experiences and 8% did not remember the last time they had sober sex. Methamphetamine, mephedrone, GHB/GBL, and having had open relationships, group sex, double penetration, and fisting were significantly more prevalent. Forty-nine percent admitted being worried about chemsex use, and 18% said they needed help. A comprehensive, interdisciplinary approach is mandatory to enable the attainment of a healthy approach to one's sex life.
    MeSH term(s) Male ; Humans ; Homosexuality, Male ; Sodium Oxybate ; Spain/epidemiology ; HIV Infections/epidemiology ; HIV Infections/prevention & control ; Pre-Exposure Prophylaxis ; Sexual Behavior ; Methamphetamine ; Substance-Related Disorders/epidemiology ; Hospitals ; Sexual and Gender Minorities
    Chemical Substances Sodium Oxybate (7G33012534) ; Methamphetamine (44RAL3456C)
    Language English
    Publishing date 2022-06-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1339885-4
    ISSN 1573-3254 ; 1090-7165
    ISSN (online) 1573-3254
    ISSN 1090-7165
    DOI 10.1007/s10461-022-03730-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6451: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Characteristics and Management of Ocular Involvement in Individuals with Monkeypox Disease.

    Pazos, Marta / Riera, Josep / Moll-Udina, Aina / Catala, Alba / Narvaez, Sofia / Fuertes, Irene / Dotti-Boada, Marina / Petiti, Gisela / Izquierdo-Serra, Jordi / Maldonado, Enrique / Chang-Sotomayor, Meilin / Garcia, David / Camós-Carreras, Anna / Gilera, Vanessa / De Loredo, Nicolas / Peraza-Nieves, Jorge / Ventura-Abreu, Nestor / Spencer, Felipe / Del Carlo, Guillerima F /
    Torras, Josep / Nicolas, Josep Maria / Adán, Alfredo / Vilella, Anna / Puig, Susana / Martinez, Esteban / Martinez, Mikel J / Sánchez-Dalmau, Bernardo / Blanco, José L

    Ophthalmology

    2023  Volume 130, Issue 6, Page(s) 655–658

    MeSH term(s) Humans ; Mpox (monkeypox) ; Eye ; Face
    Language English
    Publishing date 2023-02-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 392083-5
    ISSN 1549-4713 ; 0161-6420
    ISSN (online) 1549-4713
    ISSN 0161-6420
    DOI 10.1016/j.ophtha.2023.02.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ul II Zs.83: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 533: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Role of ghrelin in fertilization, early embryo development, and implantation periods.

    Luque, Eugenia Mercedes / Torres, Pedro Javier / de Loredo, Nicolás / Vincenti, Laura María / Stutz, Graciela / Santillán, María Emilia / Ruiz, Rubén Daniel / de Cuneo, Marta Fiol / Martini, Ana Carolina

    Reproduction (Cambridge, England)

    2014  Volume 148, Issue 2, Page(s) 159–167

    Abstract: In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (d-Lys3)GHRP6) on fertilization, early embryo ... ...

    Abstract In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (d-Lys3)GHRP6) on fertilization, early embryo development, and implantation periods in mice. Three experiments were performed, treating female mice with ghrelin or its antagonist: i) starting from 1 week before copulation to 12 h after copulation, mice were killed at day 18 of gestation; ii) since ovulation induction until 80 h later, when we retrieved the embryos from oviducts/uterus, and iii) starting from days 3 to 7 of gestation (peri-implantation), mice were killed at day 18. In experiments 1 and 3, the antagonist and/or the highest dose of ghrelin significantly increased the percentage of atrophied fetuses and that of females exhibiting this finding or a higher amount of corpora lutea compared with fetuses (nCL/nF) (experiment 3: higher nCL/nF-atrophied fetuses: ghrelin 4, 71.4-71.4% and antagonist, 75.0-62.5% vs ghrelin 2, 46.2-15.4% and control, 10-0.0%; n=7-13 females/group; P<0.01). In experiment 2, the antagonist diminished the fertilization rate, and both, ghrelin and the antagonist, delayed embryo development (blastocysts: ghrelin 2, 62.5%; ghrelin 4, 50.6%; and antagonist, 61.0% vs control 78.4%; n=82-102 embryos/treatment; P<0.0001). In experiment 3, additionally, ghrelin (4 nmol/day) and the antagonist significantly diminished the weight gain of fetuses and dams during pregnancy. Our results indicate that not only hyperghrelinemia but also the inhibition of the endogenous ghrelin effects exerts negative effects on the fertilization, implantation, and embryo/fetal development periods, supporting the hypothesis that ghrelin (in 'adequate' concentrations) has a physiological role in early gestational events.
    MeSH term(s) Animals ; Copulation ; Corpus Luteum/cytology ; Corpus Luteum/drug effects ; Embryo Implantation/drug effects ; Embryo, Mammalian/cytology ; Embryo, Mammalian/drug effects ; Female ; Fertilization/drug effects ; Fertilization/physiology ; Ghrelin/pharmacology ; Mice ; Pregnancy
    Chemical Substances Ghrelin
    Language English
    Publishing date 2014-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2034501-X
    ISSN 1741-7899 ; 1470-1626 ; 1476-3990
    ISSN (online) 1741-7899
    ISSN 1470-1626 ; 1476-3990
    DOI 10.1530/REP-14-0129
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.362: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top