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  1. Article ; Online: An overview of New Psychoactive Substances (NPS) in northeast Brazil: NMR-based identification and analysis of ecstasy tablets by GC-MS.

    Cunha, Ricardo Leal / Oliveira, Celinalva da Silva Lima / de Oliveira, Aline Lima / Maldaner, Adriano Otávio / do Desterro Cunha, Silvio / Pereira, Pedro Afonso P

    Forensic science international

    2023  Volume 344, Page(s) 111597

    Abstract: The actual illicit market for synthetic drugs is characterized by a wide variety of psychoactive substances of different chemical and pharmacological classes, such as amphetamine-type stimulants and new psychoactive substances. The knowledge about its ... ...

    Abstract The actual illicit market for synthetic drugs is characterized by a wide variety of psychoactive substances of different chemical and pharmacological classes, such as amphetamine-type stimulants and new psychoactive substances. The knowledge about its chemical composition, as well as the nature and quantity of the active substances present, is important for emergency care in intoxication cases by these substances and to establish adequate chemical and toxicological analysis procedures in forensic laboratories. The aim of this work was to study the prevalence of amphetamine-type stimulants and new psychoactive substances in the states of Bahia and Sergipe, in the northeast region of Brazil, involving samples of drugs seized by the local police forces from 2014 to 2019. In a total of 121 seized and analyzed samples, in which ecstasy tablets predominated (n = 101), nineteen substances were identified using GC-MS and
    MeSH term(s) N-Methyl-3,4-methylenedioxyamphetamine/analysis ; Brazil ; Gas Chromatography-Mass Spectrometry ; Illicit Drugs/analysis ; Central Nervous System Stimulants/analysis ; Amphetamine/analysis ; Tablets ; Synthetic Drugs ; Psychotropic Drugs/analysis
    Chemical Substances N-Methyl-3,4-methylenedioxyamphetamine (KE1SEN21RM) ; Illicit Drugs ; Central Nervous System Stimulants ; Amphetamine (CK833KGX7E) ; Tablets ; Synthetic Drugs ; Psychotropic Drugs
    Language English
    Publishing date 2023-02-03
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 424042-x
    ISSN 1872-6283 ; 0379-0738
    ISSN (online) 1872-6283
    ISSN 0379-0738
    DOI 10.1016/j.forsciint.2023.111597
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  2. Article ; Online: Probing human proteins for short encrypted antimicrobial peptides reveals Hs10, a peptide with selective activity for gram-negative bacteria.

    Santos, Michele A / Silva, Fernanda L / Lira, Bianca O V / Cardozo Fh, José L / Vasconcelos, Andreanne G / Araujo, Alyne R / Murad, André M / Garay, Aisel V / Freitas, Sonia M / Leite, José Roberto S A / Bloch, Carlos / Ramada, Marcelo H S / de Oliveira, Aline Lima / Brand, Guilherme D

    Biochimica et biophysica acta. General subjects

    2022  Volume 1867, Issue 1, Page(s) 130265

    Abstract: Background: Some cationic and amphiphilic α-helical segments of proteins adsorb to prokaryotic membranes when synthesized as individual polypeptide sequences, resulting in broad and potent antimicrobial activity. However, amphiphilicity, a determinant ... ...

    Abstract Background: Some cationic and amphiphilic α-helical segments of proteins adsorb to prokaryotic membranes when synthesized as individual polypeptide sequences, resulting in broad and potent antimicrobial activity. However, amphiphilicity, a determinant physicochemical property for peptide-membrane interactions, can also be observed in some β-sheets.
    Methods: The software Kamal was used to scan the human reference proteome for short (7-11 amino acid residues) cationic and amphiphilic protein segments with the characteristic periodicity of β-sheets. Some of the uncovered peptides were chemically synthesized, and antimicrobial assays were conducted. Biophysical techniques were used to probe the molecular interaction of one peptide with phospholipid vesicles, lipopolysaccharides (LPS) and the bacterium Escherichia coli.
    Results: Thousands of compatible segments were found in human proteins, five were synthesized, and three presented antimicrobial activity in the micromolar range. Hs10, a nonapeptide fragment of the Complement C3 protein, could inhibit only the growth of tested Gram-negative microorganisms, presenting also little cytotoxicity to human fibroblasts. Hs10 interacted with LPS while transitioning from an unstructured segment to a β-sheet and increased the hydrodynamic radius of LPS particles. This peptide also promoted morphological alterations in E. coli cells.
    Conclusions: Data presented herein introduce yet another molecular template to probe proteins in search for encrypted membrane-active segments and demonstrates that, using this approach, short peptides with low cytotoxicity and high selectivity to prokaryotic cells might be obtained.
    General significance: This work widens the biotechnological potential of the human proteome as a source of antimicrobial peptides with application in human health.
    MeSH term(s) Humans ; Escherichia coli/metabolism ; Antimicrobial Peptides ; Lipopolysaccharides/pharmacology ; Proteome ; Gram-Negative Bacteria/metabolism ; Peptides/chemistry ; Anti-Infective Agents
    Chemical Substances Antimicrobial Peptides ; Lipopolysaccharides ; Proteome ; Peptides ; Anti-Infective Agents
    Language English
    Publishing date 2022-10-21
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbagen.2022.130265
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  3. Article ; Online: Can co-application of silicate rock powder and humic-like acids increase nutrient uptake and plant growth in weathered tropical soil?

    Busato, Jader Galba / dos Santos, Luiz Fernando / Monteiro de Paula, Alessandra / Fabriz Sodré, Fernando / de Oliveira, Aline Lima / Dobbss, Leonardo Barros / de Souza Martins, Éder / Jindo, Keiji

    Acta Agriculturae Scandinavica Section B: Soil and Plant Science

    2022  Volume 72, Issue 1

    Abstract: Silicate rock powder (SRP) restores the fertility of weathered soils. However, its slow nutrient release is a disadvantage for short-duration crops. Humic-like acids (HLAs) are plant biostimulants that enhance root development and nutrient uptake. This ... ...

    Abstract Silicate rock powder (SRP) restores the fertility of weathered soils. However, its slow nutrient release is a disadvantage for short-duration crops. Humic-like acids (HLAs) are plant biostimulants that enhance root development and nutrient uptake. This work evaluates the effects of the co-application of HLA extracted from a vermicompost and SRP on the nutrient uptake and growth of maize cultivated in weathered soil in Brazil. The chemical composition of HLA was assessed using Fourier-transform infrared spectroscopy and 13Carbon-nuclear magnetic resonance, revealing an overall characteristic of hydrophobicity. A preliminary trial with different HLA concentrations (0, 20, 40, 80, and 160 mg L−1) revealed that 40 mg L−1 HLA resulted in the highest increase in the root area, dry root weight, H+ efflux, and the number of lateral roots, compared with other concentrations. The main experiment using soil treated with SRP at different rates (0, 600, 1200, 1800, and 2400 kg ha−1) showed that the co-application of SRP and HLA caused a significant difference in the root and total plant weights, compared with the sole SRP application. Furthermore, it increased the nutrient content of the plants. These effects are mainly because of increased proton pump activity and the hydrophobicity of HLA.
    Keywords Plant nutrients ; biostimulant ; humic-like acids ; latsols ; phosphorous ; silicate rock powder ; tropical soil
    Subject code 580
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 32458-9
    ISSN 1651-1913 ; 0906-4710
    ISSN (online) 1651-1913
    ISSN 0906-4710
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  4. Article ; Online: Recycling of wastes from fish beneficiation by composting: chemical characteristics of the compost and efficiency of their humic acids in stimulating the growth of lettuce.

    Busato, Jader Galba / de Carvalho, Caroline Moreira / Zandonadi, Daniel Basilio / Sodré, Fernando Fabriz / Mol, Alan Ribeiro / de Oliveira, Aline Lima / Navarro, Rodrigo Diana

    Environmental science and pollution research international

    2017  Volume 25, Issue 36, Page(s) 35811–35820

    Abstract: Waste from the beneficiation of fish was composted with crushed grass aiming to characterize their chemical composition and investigate the possibility of the use of the final compost as source of humic acids (HA) able to stimulate the growth of lettuce. ...

    Abstract Waste from the beneficiation of fish was composted with crushed grass aiming to characterize their chemical composition and investigate the possibility of the use of the final compost as source of humic acids (HA) able to stimulate the growth of lettuce. Compost presented pH value, C/N ratio, and electrical conductivity that allow its use as an organic fertilizer. The element content was present in the following order of abundance in the compost: P > Ca > N > Mg > K > Fe > Zn > Mn > Mo > Cu, and the humus composition was similar to that observed in others kind of organic residues composted. The high content of oxygen pointed out a high level of oxidation of HA, in line with the predominance of phenolic acidity in the functional groups. The
    MeSH term(s) Animals ; Composting ; Fertilizers/analysis ; Fishes ; Humic Substances/analysis ; Lactuca/growth & development ; Poaceae/chemistry ; Recycling ; Soil/chemistry ; Solid Waste/analysis
    Chemical Substances Fertilizers ; Humic Substances ; Soil ; Solid Waste
    Language English
    Publishing date 2017-11-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-017-0795-3
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  5. Article ; Online: Phosphatase activity and its relationship with physical and chemical parameters during vermicomposting of filter cake and cattle manure.

    Busato, Jader Galba / Papa, Gabriella / Canellas, Luciano Pasqualoto / Adani, Fabrizio / de Oliveira, Aline Lima / Leão, Tairone Paiva

    Journal of the science of food and agriculture

    2016  Volume 96, Issue 4, Page(s) 1223–1230

    Abstract: Background: Recycling of phosphorus (P) from organic residues (ORs) is important to develop environmentally friendly agriculture. The use of this P source depends on phosphatase enzymes, which can be affected by a chain of parameters during maturation ... ...

    Abstract Background: Recycling of phosphorus (P) from organic residues (ORs) is important to develop environmentally friendly agriculture. The use of this P source depends on phosphatase enzymes, which can be affected by a chain of parameters during maturation of ORs. In this study the phosphatase activity levels throughout vermicomposting of filter cake (FC) and cattle manure (CM) were correlated with different physical and chemical parameters in an effort to increase the knowledge about recycling of P from ORs.
    Results: FC presented higher total nitrogen content (TNC), total organic carbon (TOC), humic acid (HA) content, water-soluble P (WSP), phosphatase activities and nanopore volume than CM during vermicomposting. Decreases in TOC of CM resulted from carbohydrate mineralization, which was not observed for FC. CM showed increased hydrophobic index during vermicomposting while FC showed a slight decrease.
    Conclusion: Phosphatase activities correlated positively with TOC, pH and WSP and negatively with HA content for both vermicomposts. Nanopore volume was negatively correlated with phosphatase activities for FC but not for CM. No correlations between hydrophobicity and phosphatase activities were found for FC. Increased hydrophobicity throughout vermicomposting of CM could be partially associated with decreases in phosphatase levels.
    MeSH term(s) Acid Phosphatase/chemistry ; Animal Husbandry ; Animals ; Cattle ; Conservation of Natural Resources ; Dairying ; Female ; Humic Substances ; Manure
    Chemical Substances Humic Substances ; Manure ; Acid Phosphatase (EC 3.1.3.2)
    Language English
    Publishing date 2016-03-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 184116-6
    ISSN 1097-0010 ; 0022-5142
    ISSN (online) 1097-0010
    ISSN 0022-5142
    DOI 10.1002/jsfa.7210
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    Zs.B 808: Show issues Location:
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  6. Article: Organic matter pools and nutrient cycling in different coffee production systems in the Brazilian Cerrado

    Marinho, Eduardo Barros / de Oliveira, Aline Lima / Zandonadi, Daniel Basílio / Benedito, Luiz Eduardo Celino / de Souza, Ronessa Bartolomeu / de Figueiredo, Cícero Célio / Busato, Jader Galba

    Agroforestry systems. 2014 Oct., v. 88, no. 5

    2014  

    Abstract: Agroforestry and organic systems have been used to reduce the negative effects that conventional coffee cultivation has on soils. In this work, ¹³C-CPMAS-NMR, Fourier transform infrared spectroscopy, elemental composition, classical humus fractionation ... ...

    Abstract Agroforestry and organic systems have been used to reduce the negative effects that conventional coffee cultivation has on soils. In this work, ¹³C-CPMAS-NMR, Fourier transform infrared spectroscopy, elemental composition, classical humus fractionation and the soil fertility status were used to evaluate the impact of these three systems on a Latosol from the Brazilian Cerrado. Continuous input of tree residues promoted changes to the soil organic matter with increase in total organic carbon, humic acids (HA) and light organic matter, mainly in the topsoil. Available P and cation exchange capacity were also increased and the acidity status decreased in the agroforestry system. Moreover, HA from the agroforestry were enriched in O-alkyl C, O-di-alkyl C and alkyl C groups and the organic system resulted in HA richer in carboxyl groups. The conventional system resulted in greater aromatic and methoxyl participation, and lower phenol groups. HA from the agroforestry system were richer in easily degradable structures and the chemical fractionation demonstrated a decrease in both recalcitrant fractions, allowing for a more conservative and sustainable management of soil fertility. The modifications were not as evident in the organic system, probably due to the low organic fertilizer input.
    Keywords Fourier transform infrared spectroscopy ; acidity ; agroforestry ; biogeochemical cycles ; carbon ; cation exchange capacity ; cerrado ; chemical structure ; crop production ; elemental composition ; fractionation ; humic acids ; humus ; organic fertilizers ; phenol ; production technology ; soil fertility ; topsoil ; trees
    Language English
    Dates of publication 2014-10
    Size p. 767-778.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 406958-4
    ISSN 1572-9680 ; 0167-4366
    ISSN (online) 1572-9680
    ISSN 0167-4366
    DOI 10.1007/s10457-014-9723-4
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  7. Article ; Online: Complete assignment of NMR data of 22 phenyl-1H-pyrazoles' derivatives.

    de Oliveira, Aline Lima / Alves de Oliveira, Carlos Henrique / Mairink, Laura Maia / Pazini, Francine / Menegatti, Ricardo / Lião, Luciano Morais

    Magnetic resonance in chemistry : MRC

    2011  Volume 49, Issue 8, Page(s) 537–542

    Abstract: Complete assignment of (1)H and (13)C NMR chemical shifts and J((1)H/(1)H and (1)H/(19)F) coupling constants for 22 1-phenyl-1H-pyrazoles' derivates were performed using the concerted application of (1)H 1D and (1)H, (13)C 2D gs-HSQC and gs-HMBC ... ...

    Abstract Complete assignment of (1)H and (13)C NMR chemical shifts and J((1)H/(1)H and (1)H/(19)F) coupling constants for 22 1-phenyl-1H-pyrazoles' derivates were performed using the concerted application of (1)H 1D and (1)H, (13)C 2D gs-HSQC and gs-HMBC experiments. All 1-phenyl-1H-pyrazoles' derivatives were synthesized as described by Finar and co-workers. The formylated 1-phenyl-1H-pyrazoles' derivatives were performed under Duff's conditions.
    MeSH term(s) Carbon Isotopes ; Molecular Structure ; Nuclear Magnetic Resonance, Biomolecular ; Phenols/chemical synthesis ; Phenols/chemistry ; Protons ; Pyrazoles/chemical synthesis ; Pyrazoles/chemistry ; Reference Standards
    Chemical Substances Carbon Isotopes ; Phenols ; Protons ; Pyrazoles
    Language English
    Publishing date 2011-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1475029-6
    ISSN 1097-458X ; 0749-1581
    ISSN (online) 1097-458X
    ISSN 0749-1581
    DOI 10.1002/mrc.2773
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  8. Book ; Online: Rob\^oCIn Small Size League Extended Team Description Paper for RoboCup 2023

    de Oliveira, Aline Lima / Gomes, Cauê Addae da Silva / da Silva, Cecília Virginia Santos / Alves, Charles Matheus de Sousa / de Souza, Danilo Andrade Martins / Xavier, Driele Pires Ferreira Araújo / da Silva, Edgleyson Pereira / Martins, Felipe Bezerra / Santos, Lucas Henrique Cavalcanti / Maciel, Lucas Dias / Santos, Matheus Paixão Gumercindo dos / Vasconcelos, Matheus Lafayette / Andrade, Matheus Vinícius Teotonio do Nascimento / de Melo, João Guilherme Oliveira Carvalho / de Moura, João Pedro Souza Pereira / da Silva, José Ronald / Cruz, José Victor Silva / de Morais, Pedro Henrique Santana / de Oliveira, Pedro Paulo Salman /
    Rodrigues, Riei Joaquim Matos / Fernandes, Roberto Costa / Morais, Ryan Vinicius Santos / Teobaldo, Tamara Mayara Ramos / Silva, Washington Igor dos Santos / Barros, Edna Natividade Silva

    2023  

    Abstract: Rob\^oCIn has participated in RoboCup Small Size League since 2019, won its first world title in 2022 (Division B), and is currently a three-times Latin-American champion. This paper presents our improvements to defend the Small Size League (SSL) ... ...

    Abstract Rob\^oCIn has participated in RoboCup Small Size League since 2019, won its first world title in 2022 (Division B), and is currently a three-times Latin-American champion. This paper presents our improvements to defend the Small Size League (SSL) division B title in RoboCup 2023 in Bordeaux, France. This paper aims to share some of the academic research that our team developed over the past year. Our team has successfully published 2 articles related to SSL at two high-impact conferences: the 25th RoboCup International Symposium and the 19th IEEE Latin American Robotics Symposium (LARS 2022). Over the last year, we have been continuously migrating from our past codebase to Unification. We will describe the new architecture implemented and some points of software and AI refactoring. In addition, we discuss the process of integrating machined components into the mechanical system, our development for participating in the vision blackout challenge last year and what we are preparing for this year.
    Keywords Computer Science - Robotics ; Computer Science - Artificial Intelligence
    Publishing date 2023-07-19
    Publishing country us
    Document type Book ; Online
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  9. Article: Phosphatase activity and its relationship with physical and chemical parameters during vermicomposting of filter cake and cattle manure

    Busato, Jader Galba / Papa, Gabriella / Canellas, Luciano Pasqualoto / Adani, Fabrizio / de Oliveira, Aline Lima / Leão, Tairone Paiva

    Journal of the science of food and agriculture

    Volume v. 96,, Issue no. 4

    Abstract: BACKGROUND: Recycling of phosphorus (P) from organic residues (ORs) is important to develop environmentally friendly agriculture. The use of this P source depends on phosphatase enzymes, which can be affected by a chain of parameters during maturation of ...

    Abstract BACKGROUND: Recycling of phosphorus (P) from organic residues (ORs) is important to develop environmentally friendly agriculture. The use of this P source depends on phosphatase enzymes, which can be affected by a chain of parameters during maturation of ORs. In this study the phosphatase activity levels throughout vermicomposting of filter cake (FC) and cattle manure (CM) were correlated with different physical and chemical parameters in an effort to increase the knowledge about recycling of P from ORs. RESULTS: FC presented higher total nitrogen content (TNC), total organic carbon (TOC), humic acid (HA) content, water‐soluble P (WSP), phosphatase activities and nanopore volume than CM during vermicomposting. Decreases in TOC of CM resulted from carbohydrate mineralization, which was not observed for FC. CM showed increased hydrophobic index during vermicomposting while FC showed a slight decrease. CONCLUSION: Phosphatase activities correlated positively with TOC, pH and WSP and negatively with HA content for both vermicomposts. Nanopore volume was negatively correlated with phosphatase activities for FC but not for CM. No correlations between hydrophobicity and phosphatase activities were found for FC. Increased hydrophobicity throughout vermicomposting of CM could be partially associated with decreases in phosphatase levels. © 2015 Society of Chemical Industry
    Keywords vermicomposts ; recycling ; correlation ; phosphorus ; nanopores ; humic acids ; enzymes ; filter cake ; nitrogen content ; cattle manure ; carbon ; mineralization ; hydrophobicity ; pH ; vermicomposting
    Language English
    Document type Article
    ISSN 0022-5142
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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