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Artikel: Circulation of DENV-3 Genotype 3 during 2017 to 2018 in Delhi: A Single-Center Hospital-Based Study.

Padhi, Abhishek / Gupta, Ekta / Singh, Gaurav / Parveen, Shama / Islam, Arshi / Tarai, Bansidhar

2021 Band 14, Heft 1, Seite(n) 21–26

Abstract: ... ...

Abstract	Introduction
Sprache	Englisch
Erscheinungsdatum	2021-09-14
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	2461120-7
ISSN	0974-7826 ; 0974-2727
ISSN (online)	0974-7826
ISSN	0974-2727
DOI	10.1055/s-0041-1734017
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Emergence of deadly severe acute respiratory syndrome coronavirus-2 during 2019-2020.

Islam, Arshi / Ahmed, Anwar / Naqvi, Irshad H / Parveen, Shama

Virusdisease

2020 Band 31, Heft 2, Seite(n) 128–136

Abstract: Wuhan, the city in Hubei province in China is in the focus of global community due to the outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), formerly known as 2019-nCoV. The virus ... ...

Abstract	Wuhan, the city in Hubei province in China is in the focus of global community due to the outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), formerly known as 2019-nCoV. The virus emerged in humans from Wuhan seafood market probably via zoonotic transmission. Within a few days the virus spread its tentacles rapidly to neighboring cities in China and to different geographical regions through travelers and to some extent by human to human transmission leading to significant disease burden globally. More than 2,00,000 people (including more than 8000 deaths) have been infected with this respiratory illness across 167 countries and territories worldwide leading to a pandemic. The present review provides an outline about emergence and spread of SARS-CoV-2 from Wuhan, China in 2019-2020. We have also provided information about the classification, genome, proteins, clinical presentation of COVID-19, type of clinical specimens to be collected and diagnostic methods adopted to identify the respiratory illness. In addition we have also provided information about transmission dynamics, prevention measures and treatment options that are available at the present. Subsequently, we have given a comprehensive overview of the spread of this infection from China to the other parts of the globe. Management of the ongoing outbreak of SARS-CoV-2 encompassing surveillance, clinical, immunological, genetic and evolutionary investigations are likely to provide the desired results. Joint efforts of global scientific community are needed at this hour in terms of enhancement of research on development of accurate diagnostics, antiviral therapeutics and finally into formation of an effective vaccine against the emerging novel coronavirus.
Schlagwörter	covid19
Sprache	Englisch
Erscheinungsdatum	2020-04-08
Erscheinungsland	India
Dokumenttyp	Journal Article ; Review
ZDB-ID	2846993-8
ISSN	2347-3517 ; 2347-3584
ISSN (online)	2347-3517
ISSN	2347-3584
DOI	10.1007/s13337-020-00575-1
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Global and local evolutionary dynamics of Dengue virus serotypes 1, 3, and 4.

Islam, Arshi / Deeba, Farah / Tarai, Bansidhar / Gupta, Ekta / Naqvi, Irshad H / Abdullah, Mohd / Dohare, Ravins / Ahmed, Anwar / Almajhdi, Fahad N / Hussain, Tajamul / Parveen, Shama

Epidemiology and infection

2023 Band 151, Seite(n) e127

Abstract: Evolutionary studies on Dengue virus (DENV) in endemic regions are necessary since naturally occurring mutations may lead to genotypic variations or shifts in serotypes, which may lead to future outbreaks. Our study comprehends the evolutionary dynamics ... ...

Abstract	Evolutionary studies on Dengue virus (DENV) in endemic regions are necessary since naturally occurring mutations may lead to genotypic variations or shifts in serotypes, which may lead to future outbreaks. Our study comprehends the evolutionary dynamics of DENV, using phylogenetic, molecular clock, skyline plots, network, selection pressure, and entropy analyses based on partial CprM gene sequences. We have collected 250 samples, 161 in 2017 and 89 in 2018. Details for the 2017 samples were published in our previous article and that of 2018 are presented in this study. Further evolutionary analysis was carried out using 800 sequences, which incorporate the study and global sequences from GenBank: DENV-1 (
Mesh-Begriff(e)	Humans ; Dengue Virus/genetics ; Serogroup ; Dengue/epidemiology ; Phylogeny ; Bayes Theorem ; Genotype
Sprache	Englisch
Erscheinungsdatum	2023-06-09
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632982-2
ISSN	1469-4409 ; 0950-2688
ISSN (online)	1469-4409
ISSN	0950-2688
DOI	10.1017/S0950268823000924
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Circulation of DENV-3 Genotype 3 during 2017 to 2018 in Delhi

Abhishek Padhi / Ekta Gupta / Gaurav Singh / Shama Parveen / Arshi Islam / Bansidhar Tarai

Journal of Laboratory Physicians (2021)

A Single-Center Hospital-Based Study

2021

Abstract: Introduction Delhi is hyperendemic for dengue virus (DENV) where all the four DENV have previously been reported. A constant vigilance of circulating DENV serotypes is important in surveillance, since the introduction of a new variant to areas affected ... ...

Abstract	Introduction Delhi is hyperendemic for dengue virus (DENV) where all the four DENV have previously been reported. A constant vigilance of circulating DENV serotypes is important in surveillance, since the introduction of a new variant to areas affected by preexisting serotypes constitutes a risk factor for dengue hemorrhagic fever and dengue shock syndrome. Objectives This retrospective study was performed with an objective to determine the circulating serotype and genotype of DENV in acute phase blood samples of patients who have reported to a tertiary liver care hospital in New Delhi during the last 2 years (2017–2018). Methods The data of clinician-initiated testing for dengue nonstructural protein 1 (NS1) antigen (Ag) was searched in the institutional hospital information system. The serum sample of dengue NS1 Ag-positive cases confirmed by enzyme-linked immunosorbent assay (ELISA; PANBIO, Gyeonggi-do, ROK) and a fever duration of less than 5 days were retrieved from the laboratory archive. The DENV serotyping on these sample was performed by reverse transcriptase polymerase chain reaction (RT-PCR). Sequencing and phylogenetic analysis was done for the capsid premembrane (CprM) region to determine the genotype. Results A total of 440 acute-phase samples were received. Twenty one (4.77%) were positive for dengue NS1 Ag with a mean age of 35.1 years and male-to-female ratio of 1.1:1. Eight cases (38.09%) were positive by dengue RT-PCR and all belonged to DENV-3 serotypes. Phylogenetic tree analysis revealed DENV-3 clustered to genotype III with 100% homology with 2008 Indian subcontinent strain. Conclusion This study revealed circulation of DENV-3, genotype III in Delhi from 2017 to 2018, similar to the 2008 viral type. Virological surveillance is an important exercise to be done for viral infections with public threat and outbreak potential.
Schlagwörter	dengue ; denv-3 ; genotype 3 ; surveillance ; Medicine ; R
Thema/Rubrik (Code)	572
Sprache	Englisch
Erscheinungsdatum	2021-09-01T00:00:00Z
Verlag	Thieme Medical and Scientific Publishers Pvt. Ltd.
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel: Emergence of deadly severe acute respiratory syndrome coronavirus-2 during 2019–2020

Islam, Arshi / Ahmed, Anwar / Naqvi, Irshad H / Parveen, Shama

Virusdisease. 2020 June, v. 31, no. 2

2020

Abstract	Wuhan, the city in Hubei province in China is in the focus of global community due to the outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), formerly known as 2019-nCoV. The virus emerged in humans from Wuhan seafood market probably via zoonotic transmission. Within a few days the virus spread its tentacles rapidly to neighboring cities in China and to different geographical regions through travelers and to some extent by human to human transmission leading to significant disease burden globally. More than 2,00,000 people (including more than 8000 deaths) have been infected with this respiratory illness across 167 countries and territories worldwide leading to a pandemic. The present review provides an outline about emergence and spread of SARS-CoV-2 from Wuhan, China in 2019–2020. We have also provided information about the classification, genome, proteins, clinical presentation of COVID-19, type of clinical specimens to be collected and diagnostic methods adopted to identify the respiratory illness. In addition we have also provided information about transmission dynamics, prevention measures and treatment options that are available at the present. Subsequently, we have given a comprehensive overview of the spread of this infection from China to the other parts of the globe. Management of the ongoing outbreak of SARS-CoV-2 encompassing surveillance, clinical, immunological, genetic and evolutionary investigations are likely to provide the desired results. Joint efforts of global scientific community are needed at this hour in terms of enhancement of research on development of accurate diagnostics, antiviral therapeutics and finally into formation of an effective vaccine against the emerging novel coronavirus.
Schlagwörter	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; burden of disease ; cities ; diagnostic techniques ; genome ; humans ; markets ; monitoring ; pandemic ; people ; proteins ; respiratory tract diseases ; seafoods ; therapeutics ; vaccines ; viruses ; China
Sprache	Englisch
Erscheinungsverlauf	2020-06
Umfang	p. 128-136.
Erscheinungsort	Springer India
Dokumenttyp	Artikel
Anmerkung	Review
ZDB-ID	2846993-8
ISSN	2347-3517 ; 2347-3584
ISSN (online)	2347-3517
ISSN	2347-3584
DOI	10.1007/s13337-020-00575-1
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: Emergence of deadly severe acute respiratory syndrome coronavirus-2 during 2019-2020

Islam, Arshi / Ahmed, Anwar / Naqvi, Irshad H / Parveen, Shama

Virusdisease

Abstract	Wuhan, the city in Hubei province in China is in the focus of global community due to the outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), formerly known as 2019-nCoV. The virus emerged in humans from Wuhan seafood market probably via zoonotic transmission. Within a few days the virus spread its tentacles rapidly to neighboring cities in China and to different geographical regions through travelers and to some extent by human to human transmission leading to significant disease burden globally. More than 2,00,000 people (including more than 8000 deaths) have been infected with this respiratory illness across 167 countries and territories worldwide leading to a pandemic. The present review provides an outline about emergence and spread of SARS-CoV-2 from Wuhan, China in 2019-2020. We have also provided information about the classification, genome, proteins, clinical presentation of COVID-19, type of clinical specimens to be collected and diagnostic methods adopted to identify the respiratory illness. In addition we have also provided information about transmission dynamics, prevention measures and treatment options that are available at the present. Subsequently, we have given a comprehensive overview of the spread of this infection from China to the other parts of the globe. Management of the ongoing outbreak of SARS-CoV-2 encompassing surveillance, clinical, immunological, genetic and evolutionary investigations are likely to provide the desired results. Joint efforts of global scientific community are needed at this hour in terms of enhancement of research on development of accurate diagnostics, antiviral therapeutics and finally into formation of an effective vaccine against the emerging novel coronavirus.
Schlagwörter	covid19
Verlag	WHO
Dokumenttyp	Artikel
Anmerkung	WHO #Covidence: #42021
Datenquelle	COVID19

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Artikel: Circulation of DENV-3 Genotype 3 during 2017 to 2018 in Delhi: A Single-Center Hospital-Based Study

Padhi, Abhishek / Gupta, Ekta / Singh, Gaurav / Parveen, Shama / Islam, Arshi / Tarai, Bansidhar

Journal of Laboratory Physicians

2021 Band 14, Heft 01, Seite(n) 21–26

Abstract: Introduction: Delhi is hyperendemic for dengue virus (DENV) where all the four DENV have previously been reported. A constant vigilance of circulating DENV serotypes is important in surveillance, since the introduction of a new variant to areas affected ...

Abstract	Introduction: Delhi is hyperendemic for dengue virus (DENV) where all the four DENV have previously been reported. A constant vigilance of circulating DENV serotypes is important in surveillance, since the introduction of a new variant to areas affected by preexisting serotypes constitutes a risk factor for dengue hemorrhagic fever and dengue shock syndrome. Objectives: This retrospective study was performed with an objective to determine the circulating serotype and genotype of DENV in acute phase blood samples of patients who have reported to a tertiary liver care hospital in New Delhi during the last 2 years (2017–2018). Methods: The data of clinician-initiated testing for dengue nonstructural protein 1 (NS1) antigen (Ag) was searched in the institutional hospital information system. The serum sample of dengue NS1 Ag-positive cases confirmed by enzyme-linked immunosorbent assay (ELISA; PANBIO, Gyeonggi-do, ROK) and a fever duration of less than 5 days were retrieved from the laboratory archive. The DENV serotyping on these sample was performed by reverse transcriptase polymerase chain reaction (RT-PCR). Sequencing and phylogenetic analysis was done for the capsid premembrane (CprM) region to determine the genotype. Results: A total of 440 acute-phase samples were received. Twenty one (4.77%) were positive for dengue NS1 Ag with a mean age of 35.1 years and male-to-female ratio of 1.1:1. Eight cases (38.09%) were positive by dengue RT-PCR and all belonged to DENV-3 serotypes. Phylogenetic tree analysis revealed DENV-3 clustered to genotype III with 100% homology with 2008 Indian subcontinent strain. Conclusion: This study revealed circulation of DENV-3, genotype III in Delhi from 2017 to 2018, similar to the 2008 viral type. Virological surveillance is an important exercise to be done for viral infections with public threat and outbreak potential.
Schlagwörter	dengue ; DENV-3 ; genotype 3 ; surveillance
Sprache	Englisch
Erscheinungsdatum	2021-09-14
Verlag	Thieme Medical and Scientific Publishers Pvt. Ltd.
Erscheinungsort	Stuttgart ; New York
Dokumenttyp	Artikel
ZDB-ID	2461120-7
ISSN	0974-7826 ; 0974-2727 ; 0974-7826
ISSN (online)	0974-7826
ISSN	0974-2727 ; 0974-7826
DOI	10.1055/s-0041-1734017
Datenquelle	Thieme Verlag

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Artikel ; Online: Circulation of dengue virus serotypes in hyperendemic region of New Delhi, India during 2011-2017.

Islam, Arshi / Abdullah, Mohd / Tazeen, Ayesha / Naqvi, Irshad H / Kazim, Syed Naqui / Ahmed, Anwar / Alamery, Salman Freeh / Malik, Ajamaluddin / Parveen, Shama

Journal of infection and public health

2020 Band 13, Heft 12, Seite(n) 1912–1919

Abstract: Background: Dengue fever has become a hampering menace in New Delhi India, since the disease has become hyperendemic, due to circulation of multiple serotypes of dengue virus (DENV). This hyperendemicity poses a greater risk of secondary infections in ... ...

Abstract	Background: Dengue fever has become a hampering menace in New Delhi India, since the disease has become hyperendemic, due to circulation of multiple serotypes of dengue virus (DENV). This hyperendemicity poses a greater risk of secondary infections in human health system. This is a major issue which leads to apprehension amongst the researchers and health organizations and thus requires regular epidemiological surveillance. Methods: We analyzed the prevalence and serotypic distribution of dengue fever cases reported from the Southern part of New Delhi during continued surveillance from 2011 to 2017. The blood samples for the investigation were obtained from the patients suspected with dengue fever attending the OPD at a local Health Centre. The data for 2011-2016 was already published from our laboratory. The samples collected during 2017 were serotyped and characterized in the present study. Results: A total of 565 samples (59%) were positive for DENV of 958 samples tested by RT-PCR during 7 years (2011-2017). Our study has shown that most infections were caused by DENV-2 during 2011-2015. The data has shown occurrence of all four serotypes of DENV during 2015 and predominance of DENV-3 in 2016 and 2017. Further, predominant combination of DENV-1 and DENV-2 was found in most of the co-infections. To the best of our knowledge this is the first study showing the epidemiological trend of dengue fever in reference to the circulating DENV serotypes and co-infections from a hyperendemic region of New Delhi during 2011-2017. Conclusions: This hyperendemic pattern of DENV and instantaneous shift in circulation of its serotypes is likely pose a greater risk of secondary infections. Inclusion of comprehensive community and hospital surveillance of dengue fever will assist in formulation and implementation of effective control measures.
Mesh-Begriff(e)	Dengue/epidemiology ; Dengue Virus/genetics ; Genotype ; Humans ; India/epidemiology ; Serogroup
Sprache	Englisch
Erscheinungsdatum	2020-11-02
Erscheinungsland	England
Dokumenttyp	Journal Article
ISSN	1876-035X
ISSN (online)	1876-035X
DOI	10.1016/j.jiph.2020.10.009
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Circulation of dengue virus serotypes in hyperendemic region of New Delhi, India during 2011–2017

Arshi Islam / Mohd. Abdullah / Ayesha Tazeen / Irshad H. Naqvi / Syed Naqui Kazim / Anwar Ahmed / Salman Freeh Alamery / Ajamaluddin Malik / Shama Parveen

Journal of Infection and Public Health, Vol 13, Iss 12, Pp 1912-

2020 Band 1919

Abstract	Background: Dengue fever has become a hampering menace in New Delhi India, since the disease has become hyperendemic, due to circulation of multiple serotypes of dengue virus (DENV). This hyperendemicity poses a greater risk of secondary infections in human health system. This is a major issue which leads to apprehension amongst the researchers and health organizations and thus requires regular epidemiological surveillance. Methods: We analyzed the prevalence and serotypic distribution of dengue fever cases reported from the Southern part of New Delhi during continued surveillance from 2011 to 2017. The blood samples for the investigation were obtained from the patients suspected with dengue fever attending the OPD at a local Health Centre. The data for 2011–2016 was already published from our laboratory. The samples collected during 2017 were serotyped and characterized in the present study. Results: A total of 565 samples (59%) were positive for DENV of 958 samples tested by RT-PCR during 7 years (2011–2017). Our study has shown that most infections were caused by DENV-2 during 2011−2015. The data has shown occurrence of all four serotypes of DENV during 2015 and predominance of DENV-3 in 2016 and 2017. Further, predominant combination of DENV-1 and DENV-2 was found in most of the co-infections. To the best of our knowledge this is the first study showing the epidemiological trend of dengue fever in reference to the circulating DENV serotypes and co-infections from a hyperendemic region of New Delhi during 2011–2017. Conclusions: This hyperendemic pattern of DENV and instantaneous shift in circulation of its serotypes is likely pose a greater risk of secondary infections. Inclusion of comprehensive community and hospital surveillance of dengue fever will assist in formulation and implementation of effective control measures.
Schlagwörter	Co-infection ; Dengue virus (DENV) ; Epidemiology ; Hyperendemicity ; Serotypes ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
Thema/Rubrik (Code)	630
Sprache	Englisch
Erscheinungsdatum	2020-12-01T00:00:00Z
Verlag	Elsevier
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Circulation of single serotype of Dengue Virus (DENV-3) in New Delhi, India during 2016

Nazish Parveen / Arshi Islam / Ayesha Tazeen / Malik Hisamuddin / Mohd Abdullah / Irshad H. Naqvi / Md I. Faizan / Divya Gulyani / Anwar Ahmed / Shama Parveen

Journal of Infection and Public Health, Vol 12, Iss 1, Pp 49-

A change in the epidemiological trend

2019 Band 56

Abstract: Background: Dengue is a rapidly emerging arthropod borne viral infection affecting tropical and sub-tropical regions of the world. Dengue is an acute febrile illness but sometimes causes more fatal complications like dengue haemorrhagic fever (DHF) and ... ...

Abstract	Background: Dengue is a rapidly emerging arthropod borne viral infection affecting tropical and sub-tropical regions of the world. Dengue is an acute febrile illness but sometimes causes more fatal complications like dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Delhi, the capital of India has become hyper endemic for dengue virus because all the four serotypes are circulating here. Methods: The present study describes the identification of dengue virus from clinical samples collected from the suspected dengue patients from New Delhi, India during 2016. The CprM region of Dengue virus genome was analyzed for phylogenetic, selection pressure and Shannon entropy analyses. Results: The present study reports circulation of a single serotype (DENV-3) in New Delhi, during 2016. The phylogenetic analysis revealed that Indian subcontinent (genotype III) of DENV-3 was circulating in Delhi during this period. Neutral selection pressure in the analyzed region revealed relatively conserved nature of this part of the Dengue virus genome. Amino acid at 31 was positively selected and had high entropy value suggesting probability of variation at this position. Conclusions: The changing trend in circulation of dengue virus serotypes necessitates the continuous epidemiological surveillance for the dengue outbreaks in this region. Keywords: Dengue virus, Epidemiology, Phylogenetic analysis, Selection pressure, Entropy analysis
Schlagwörter	Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
Sprache	Englisch
Erscheinungsdatum	2019-01-01T00:00:00Z
Verlag	Elsevier
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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