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  1. Artikel ; Online: Cannabinoid Receptor Type 1 Activation Causes a Water Diuresis by Inducing an Acute Central Diabetes Insipidus in Mice.

    Rein, Joshua L / Mackie, Ken / Kleyman, Thomas R / Satlin, Lisa M

    American journal of physiology. Renal physiology

    2024  

    Abstract: Cannabis and synthetic cannabinoid consumption is increasing worldwide. Cannabis contains numerous phytocannabinoids that act on the G-protein-coupled cannabinoid receptors type 1 (CB1R) and type 2 (CB2R) expressed throughout the body, including the ... ...

    Abstract Cannabis and synthetic cannabinoid consumption is increasing worldwide. Cannabis contains numerous phytocannabinoids that act on the G-protein-coupled cannabinoid receptors type 1 (CB1R) and type 2 (CB2R) expressed throughout the body, including the kidney. Essentially every organ, including the kidney, produces endocannabinoids (ECs), endogenous ligands to these receptors. Cannabinoids acutely increase urine output in rodents and humans, thus potentially influencing total-body water and electrolyte homeostasis. As the kidney collecting duct (CD) regulates total body water, acid/base, and electrolyte balance through specific functions of principal cells (PCs) and intercalated cells (ICs), we examined the cell-specific immunolocalization of CB1R in the mouse CD. Antibodies against either the C-terminus or N-terminus of CB1R consistently labeled AQP2(-) cells in the cortical and medullary CD, and thus presumably ICs. Given the well-established role of ICs in urinary acidification, we utilized a clearance approach in mice that were acid-loaded with 280 mM NH
    Sprache Englisch
    Erscheinungsdatum 2024-04-18
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 603837-2
    ISSN 1522-1466 ; 0363-6127
    ISSN (online) 1522-1466
    ISSN 0363-6127
    DOI 10.1152/ajprenal.00320.2022
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Navigating the kidney organoid: insights into assessment and enhancement of nephron function.

    Tabibzadeh, Nahid / Satlin, Lisa M / Jain, Sanjay / Morizane, Ryuji

    American journal of physiology. Renal physiology

    2023  Band 325, Heft 6, Seite(n) F695–F706

    Abstract: Kidney organoids are three-dimensional structures generated from pluripotent stem cells (PSCs) that are capable of recapitulating the major structures of mammalian kidneys. As this technology is expected to be a promising tool for studying renal biology, ...

    Abstract Kidney organoids are three-dimensional structures generated from pluripotent stem cells (PSCs) that are capable of recapitulating the major structures of mammalian kidneys. As this technology is expected to be a promising tool for studying renal biology, drug discovery, and regenerative medicine, the functional capacity of kidney organoids has emerged as a critical question in the field. Kidney organoids produced using several protocols harbor key structures of native kidneys. Here, we review the current state, recent advances, and future challenges in the functional characterization of kidney organoids, strategies to accelerate and enhance kidney organoid functions, and access to PSC resources to advance organoid research. The strategies to construct physiologically relevant kidney organoids include the use of organ-on-a-chip technologies that integrate fluid circulation and improve organoid maturation. These approaches result in increased expression of the major tubular transporters and elements of mechanosensory signaling pathways suggestive of improved functionality. Nevertheless, continuous efforts remain crucial to create kidney tissue that more faithfully replicates physiological conditions for future applications in kidney regeneration medicine and their ethical use in patient care.
    Mesh-Begriff(e) Animals ; Humans ; Kidney/physiology ; Regeneration ; Nephrons ; Pluripotent Stem Cells ; Organoids/metabolism ; Mammals
    Sprache Englisch
    Erscheinungsdatum 2023-09-28
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 603837-2
    ISSN 1522-1466 ; 0363-6127
    ISSN (online) 1522-1466
    ISSN 0363-6127
    DOI 10.1152/ajprenal.00166.2023
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  3. Artikel ; Online: Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca

    Carrisoza-Gaytan, Rolando / Kroll, Katharina T / Hiratsuka, Ken / Gupta, Navin R / Morizane, Ryuji / Lewis, Jennifer A / Satlin, Lisa M

    American journal of physiology. Cell physiology

    2023  Band 324, Heft 3, Seite(n) C757–C768

    Abstract: ... Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, Miyoshi T, Mau D, Valerius MT, Ferrante T ...

    Abstract Kidney organoids cultured on adherent matrices in the presence of superfusate flow generate vascular networks and exhibit more mature podocyte and tubular compartments compared with static controls (Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, Miyoshi T, Mau D, Valerius MT, Ferrante T, Bonventre JV, Lewis JA, Morizane R.
    Mesh-Begriff(e) Animals ; Mice ; Calcium/metabolism ; Fura-2 ; Ion Channels/metabolism ; Kidney/metabolism ; Kidney Tubules/metabolism ; Calcium Signaling
    Chemische Substanzen Calcium (SY7Q814VUP) ; Fura-2 (TSN3DL106G) ; Ion Channels ; Piezo1 protein, mouse
    Sprache Englisch
    Erscheinungsdatum 2023-02-06
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 392098-7
    ISSN 1522-1563 ; 0363-6143
    ISSN (online) 1522-1563
    ISSN 0363-6143
    DOI 10.1152/ajpcell.00288.2022
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract.

    Dalghi, Marianela G / DuRie, Ella / Ruiz, Wily G / Clayton, Dennis R / Montalbetti, Nicolas / Mutchler, Stephanie B / Satlin, Lisa M / Kleyman, Thomas R / Carattino, Marcelo D / Shi, Yun Stone / Apodaca, Gerard

    Physiological reports

    2024  Band 12, Heft 9, Seite(n) e16043

    Abstract: The epithelial cells that line the kidneys and lower urinary tract are exposed to mechanical forces including shear stress and wall tension; however, the mechanosensors that detect and respond to these stimuli remain obscure. Candidates include the OSCA/ ... ...

    Abstract The epithelial cells that line the kidneys and lower urinary tract are exposed to mechanical forces including shear stress and wall tension; however, the mechanosensors that detect and respond to these stimuli remain obscure. Candidates include the OSCA/TMEM63 family of ion channels, which can function as mechanosensors and osmosensors. Using Tmem63b
    Mesh-Begriff(e) Animals ; Mice ; Male ; Female ; Urinary Tract/metabolism ; Mechanotransduction, Cellular/physiology ; Ion Channels/metabolism ; Ion Channels/genetics ; Mice, Inbred C57BL ; Urothelium/metabolism ; Urothelium/cytology ; Epithelial Cells/metabolism
    Chemische Substanzen Ion Channels
    Sprache Englisch
    Erscheinungsdatum 2024-05-09
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2724325-4
    ISSN 2051-817X ; 2051-817X
    ISSN (online) 2051-817X
    ISSN 2051-817X
    DOI 10.14814/phy2.16043
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: PIEZO1 is a distal nephron mechanosensor and is required for flow-induced K+ secretion.

    Carrisoza-Gaytan, Rolando / Mutchler, Stephanie M / Carattino, Francisco / Soong, Joanne / Dalghi, Marianela G / Wu, Peng / Wang, WenHui / Apodaca, Gerard / Satlin, Lisa M / Kleyman, Thomas R

    The Journal of clinical investigation

    2024  Band 134, Heft 5

    Abstract: Ca2+-activated BK channels in renal intercalated cells (ICs) mediate luminal flow-induced K+ secretion (FIKS), but how ICs sense increased flow remains uncertain. We examined whether PIEZO1, a mechanosensitive Ca2+-permeable channel expressed in the ... ...

    Abstract Ca2+-activated BK channels in renal intercalated cells (ICs) mediate luminal flow-induced K+ secretion (FIKS), but how ICs sense increased flow remains uncertain. We examined whether PIEZO1, a mechanosensitive Ca2+-permeable channel expressed in the basolateral membranes of ICs, is required for FIKS. In isolated cortical collecting ducts (CCDs), the mechanosensitive cation-selective channel inhibitor GsMTx4 dampened flow-induced increases in intracellular Ca2+ concentration ([Ca2+]i), whereas the PIEZO1 activator Yoda1 increased [Ca2+]i and BK channel activity. CCDs from mice fed a high-K+ (HK) diet exhibited a greater Yoda1-dependent increase in [Ca2+]i than CCDs from mice fed a control K+ diet. ICs in CCDs isolated from mice with a targeted gene deletion of Piezo1 in ICs (IC-Piezo1-KO) exhibited a blunted [Ca2+]i response to Yoda1 or increased flow, with an associated loss of FIKS in CCDs. Male IC-Piezo1-KO mice selectively exhibited an increased blood [K+] in response to an oral K+ bolus and blunted urinary K+ excretion following a volume challenge. Whole-cell expression of BKα subunit was reduced in ICs of IC-Piezo1-KO mice fed an HK diet. We conclude that PIEZO1 mediates flow-induced basolateral Ca2+ entry into ICs, is upregulated in the CCD in response to an HK diet, and is necessary for FIKS.
    Mesh-Begriff(e) Male ; Mice ; Animals ; Kidney Tubules, Collecting/metabolism ; Large-Conductance Calcium-Activated Potassium Channels/genetics ; Large-Conductance Calcium-Activated Potassium Channels/metabolism ; Calcium/metabolism ; Nephrons/metabolism ; Kidney/metabolism ; Ion Channels/genetics ; Ion Channels/metabolism
    Chemische Substanzen Large-Conductance Calcium-Activated Potassium Channels ; Calcium (SY7Q814VUP) ; Piezo1 protein, mouse ; Ion Channels
    Sprache Englisch
    Erscheinungsdatum 2024-03-01
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 3067-3
    ISSN 1558-8238 ; 0021-9738
    ISSN (online) 1558-8238
    ISSN 0021-9738
    DOI 10.1172/JCI174806
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Influence of Proteolytic Cleavage of ENaC's Gamma Subunit upon Na

    Ray, Evan C / Nickerson, Andrew / Sheng, Shaohu / Carrisoza-Gaytán, Rolando / Lam, Tracey / Marciszyn, Allison / Zhang, Lei / Jordahl, Alexa / Bi, Chunming / Winfrey, Aaliyah / Kou, Zhaohui / Gingras, Sebastien / Kirabo, Annet / Satlin, Lisa M / Kleyman, Thomas R

    American journal of physiology. Renal physiology

    2024  

    Abstract: The ENaC γ subunit is essential for homeostasis of ... ...

    Abstract The ENaC γ subunit is essential for homeostasis of Na
    Sprache Englisch
    Erscheinungsdatum 2024-04-18
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 603837-2
    ISSN 1522-1466 ; 0363-6127
    ISSN (online) 1522-1466
    ISSN 0363-6127
    DOI 10.1152/ajprenal.00027.2024
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  7. Artikel: Proteolytic Cleavage of the ENaC γ Subunit - Impact Upon Na

    Ray, Evan C / Nickerson, Andrew / Sheng, Shaohu / Carrisoza-Gaytan, Rolando / Lam, Tracey / Marciszyn, Allison / Zhang, Lei / Jordahl, Alexa / Bi, Chunming / Winfrey, Aaliyah / Kou, Zhaohui / Gingras, Sebastien / Kirabo, Annet / Satlin, Lisa M / Kleyman, Thomas R

    bioRxiv : the preprint server for biology

    2024  

    Abstract: The ENaC gamma subunit is essential for homeostasis of ... ...

    Abstract The ENaC gamma subunit is essential for homeostasis of Na
    Sprache Englisch
    Erscheinungsdatum 2024-02-14
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2024.02.12.579964
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel: Mineralocorticoid receptor-independent activation of ENaC in bile duct ligated mice.

    Wang, Xue-Ping / Mutchler, Stephanie M / Carrisoza-Gáytan, Rolando / Al-Bataineh, Mohammad / Baty, Catherine J / Vandevender, Amber / Srinivasan, Priyanka / Tan, Roderick J / Jurczak, Michael J / Satlin, Lisa M / Kashlan, Ossama B

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Sodium and fluid retention in liver disease is classically thought to result from reduced effective circulating volume and stimulation of the renin-angiotensin-aldosterone system (RAAS). Aldosterone dives ... ...

    Abstract Sodium and fluid retention in liver disease is classically thought to result from reduced effective circulating volume and stimulation of the renin-angiotensin-aldosterone system (RAAS). Aldosterone dives Na
    Sprache Englisch
    Erscheinungsdatum 2023-09-22
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.09.19.558474
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  9. Artikel: Live functional assays reveal longitudinal maturation of transepithelial transport in kidney organoids.

    Rizki-Safitri, Astia / Gupta, Navin / Hiratsuka, Ken / Kobayashi, Kenichi / Zhang, Chengcheng / Ida, Kazumi / Satlin, Lisa M / Morizane, Ryuji

    Frontiers in cell and developmental biology

    2022  Band 10, Seite(n) 978888

    Abstract: Kidney organoids derived from hPSCs have opened new opportunities to develop kidney models for preclinical studies and immunocompatible kidney tissues for regeneration. Organoids resemble native nephrons that consist of filtration units and tubules, yet ... ...

    Abstract Kidney organoids derived from hPSCs have opened new opportunities to develop kidney models for preclinical studies and immunocompatible kidney tissues for regeneration. Organoids resemble native nephrons that consist of filtration units and tubules, yet little is known about the functional capacity of these organoid structures. Transcriptomic analyses provide insight into maturation and transporter activities that represent kidney functions. However, functional assays in organoids are necessary to demonstrate the activity of these transport proteins in live tissues. The three-dimensional (3D) architecture adds complexity to real-time assays in kidney organoids. Here, we develop a functional assay using live imaging to assess transepithelial transport of rhodamine 123 (Rh123), a fluorescent substrate of P-glycoprotein (P-gp), in organoids affixed to coverslip culture plates for accurate real-time observation. The identity of organoid structures was probed using Lotus Tetragonolobus Lectin (LTL), which binds to glycoproteins present on the surface of proximal tubules. Within 20 min of the addition of Rh123 to culture media, Rh123 accumulated in the tubular lumen of organoids. Basolateral-to-apical accumulation of the dye/marker was reduced by pharmacologic inhibition of MDR1 or OCT2, and OCT2 inhibition reduced the Rh123 uptake. The magnitude of Rh123 transport was maturation-dependent, consistent with MDR1 expression levels assessed by RNA-seq and immunohistochemistry. Specifically, organoids on day 21 exhibit less accumulation of Rh123 in the lumen unlike later-stage organoids from day 30 of differentiation. Our work establishes a live functional assessment in 3D kidney organoids, enabling the functional phenotyping of organoids in health and disease.
    Sprache Englisch
    Erscheinungsdatum 2022-08-15
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2022.978888
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  10. Artikel ; Online: Perinatal and childhood exposure to environmental chemicals and blood pressure in children: a review of literature 2007-2017.

    Sanders, Alison P / Saland, Jeffrey M / Wright, Robert O / Satlin, Lisa

    Pediatric research

    2018  Band 84, Heft 2, Seite(n) 165–180

    Abstract: Exposure to environmental chemicals during periods of renal development from embryogenesis to birth and through childhood can inform critical windows of nephrotoxicity, including changes in childhood blood pressure. This review assessed recent studies ... ...

    Abstract Exposure to environmental chemicals during periods of renal development from embryogenesis to birth and through childhood can inform critical windows of nephrotoxicity, including changes in childhood blood pressure. This review assessed recent studies that examined the relationship of air pollution, metals, and other organic pollutants with children's blood pressure outcomes. We restricted this review to peer-reviewed studies published in English between January 2007 and July 2017. We identified a total of 36 articles that estimated associations with childhood blood pressure, of which 14 studies examined the effects of air pollution, 10 examined metals, and 12 examined other organic pollutants including phthalates (n = 4), Bisphenol A (n = 3), polychlorinated biphenols (n = 2), organophosphate pesticides (n = 2), or perfluoroalkyl acids (n = 1). Similar to the established relationship between tobacco smoke exposure and childhood blood pressure, the majority of studies that examined air pollutants, particularly exposure to PM
    Mesh-Begriff(e) Acids/analysis ; Air Pollutants/adverse effects ; Benzhydryl Compounds/analysis ; Blood Pressure/drug effects ; Child, Preschool ; Environmental Exposure ; Female ; Humans ; Hypertension/etiology ; Infant ; Infant, Newborn ; Male ; Maternal Exposure ; Metals/adverse effects ; Organic Chemicals/analysis ; Organophosphates/analysis ; Pesticides/analysis ; Phenols/analysis ; Phthalic Acids/analysis ; Polychlorinated Biphenyls/analysis ; Pregnancy
    Chemische Substanzen Acids ; Air Pollutants ; Benzhydryl Compounds ; Metals ; Organic Chemicals ; Organophosphates ; Pesticides ; Phenols ; Phthalic Acids ; Polychlorinated Biphenyls (DFC2HB4I0K) ; bisphenol A (MLT3645I99)
    Sprache Englisch
    Erscheinungsdatum 2018-06-09
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 4411-8
    ISSN 1530-0447 ; 0031-3998
    ISSN (online) 1530-0447
    ISSN 0031-3998
    DOI 10.1038/s41390-018-0055-3
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