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Artikel ; Online: Optimization of quenched fluorescent peptide substrates of SARS-CoV-2 3CL

Cesar Ramos de Jesus, Hugo / Solis, Nestor / Machado, Yoan / Pablos, Isabel / Bell, Peter A / Kappelhoff, Reinhild / Grin, Peter M / Sorgi, Carlos A / Butler, Georgina S / Overall, Christopher M

Journal of virology

2024 , Seite(n) e0004924

Abstract: SARS-CoV-2 3C-like main protease ( ... ...

Abstract	SARS-CoV-2 3C-like main protease (3CL
Sprache	Englisch
Erscheinungsdatum	2024-05-14
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/jvi.00049-24
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Assessing the efficacy of novel and conventional disinfectants on Salmonella cross contamination during washing of fresh-cut lettuce and their impact on product shelf life

Pablos, Cristina / Romero, Aitor / de Diego, Ana / Corrales, Carla / van Grieken, Rafael / Bascón, Isabel / Pérez-Rodríguez, Fernando / Marugán, Javier

Lebensmittel-Wissenschaft + Technologie. 2022 June 01, v. 162

2022

Abstract: The effect of the application of different disinfectants on the microbial load and sensory quality of fresh-cut lettuce was evaluated during washing, and after subsequent storage at 4 °C under modified atmosphere packaging (MAP). Different families of ... ...

Abstract	The effect of the application of different disinfectants on the microbial load and sensory quality of fresh-cut lettuce was evaluated during washing, and after subsequent storage at 4 °C under modified atmosphere packaging (MAP). Different families of potential alternative sanitizers were tested: quaternary ammonium compounds (QACs) as benzalkonium chloride (BZK), and didecyldimethylammonium chloride (DDAC); isothiazolinones (mixture of chloromethylisothiazolinone and methylisothiazolinone, CMIT:MIT 3:1); and, essential oils (carvacrol, CAR). All these disinfectants were effective to inactivate Salmonella (10³ CFU/mL) present in wash water. In addition, all tested chemicals could reduce Salmonella on produce to levels >95%, with chlorine and BZK-CAR reaching maximum reductions of 99.0%. These disinfectants also enhanced a reduction in natural microbiota present on the produce. The highest reduction corresponded to free chlorine (50 mg/L) (95.1%), CMIT:MIT (50 mg/L) (Kathon®) (94.5%), and BZK (300 mg/L) (91.3%). However, only free chlorine (50 mg/L), CMIT:MIT (50 mg/L) (Kathon®), and DDAC (100 mg/L) resulted in minimal negative impact on end-product quality during 14-day storage. On the contrary, an adequate sensory quality could be only maintained up to 7 days for produce treated with BZK (300 mg/L).
Schlagwörter	Salmonella ; benzalkonium chloride ; carvacrol ; chlorides ; chlorine ; cross contamination ; lettuce ; microbial load ; microorganisms ; quaternary ammonium compounds ; sensory properties ; shelf life
Sprache	Englisch
Erscheinungsverlauf	2022-0601
Erscheinungsort	Elsevier Ltd
Dokumenttyp	Artikel
ZDB-ID	241369-3
ISSN	0460-1173 ; 0023-6438
ISSN	0460-1173 ; 0023-6438
DOI	10.1016/j.lwt.2022.113441
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: Beyond BMI: cardiometabolic measures as predictors of impulsivity and white matter changes in adolescents.

Prunell-Castañé, Anna / Jurado, María Ángeles / Ottino-González, Jonatan / Prats-Soteras, Xavier / Sánchez Garre, Consuelo / Cano Marco, Neus / Salas Gómez-Pablos, Paloma / García-García, Isabel / Garolera, Maite

Brain structure & function

2023 Band 228, Heft 3-4, Seite(n) 751–760

Abstract: Obesity is characterized by cardiometabolic and neurocognitive changes. However, how these two factors relate to each other in this population is unknown. We tested the association that cardiometabolic measures may have with impulse behaviors and white ... ...

Abstract	Obesity is characterized by cardiometabolic and neurocognitive changes. However, how these two factors relate to each other in this population is unknown. We tested the association that cardiometabolic measures may have with impulse behaviors and white matter microstructure in adolescents with and without an excess weight. One hundred and eight adolescents (43 normal-weight and 65 overweight/obesity; 11-19 years old) were medically and psychologically (Temperament Character Inventory Revised, Three-Factor Eating Questionnaire-R18, Conners' Continuous Performance Test-II, Stroop Color and Word Test, Wisconsin Card Sorting Test, Kirby Delay Discounting Task) evaluated. A subsample of participants (n = 56) underwent a brain magnetic resonance imaging acquisition. In adolescents, higher triglycerides and having a body mass index indicative of overweight/obesity predicted a more impulsive performance in Conners' Continuous Performance Test-II (higher commission errors). In addition, higher glucose and diastolic blood pressure values predicted increments in the Three-Factor Eating Questionnaire-R18 emotional eating scale. Neuroanatomically, cingulum fractional anisotropy showed a negative relationship with glycated hemoglobin. The evaluation of the neurocognitive differences associated with obesity, usually based on body mass index, should be complemented with cardiometabolic measures.
Mesh-Begriff(e)	Humans ; Adolescent ; Child ; Young Adult ; Adult ; Body Mass Index ; Overweight/pathology ; White Matter/diagnostic imaging ; White Matter/pathology ; Obesity/diagnostic imaging ; Obesity/pathology ; Impulsive Behavior ; Cardiovascular Diseases/pathology
Sprache	Englisch
Erscheinungsdatum	2023-02-13
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	2273162-3
ISSN	1863-2661 ; 1863-2653
ISSN (online)	1863-2661
ISSN	1863-2653
DOI	10.1007/s00429-023-02615-0
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Enhancing Osteoblastic Cell Cultures with Gelatin Methacryloyl, Bovine Lactoferrin, and Bioactive Mesoporous Glass Scaffolds Loaded with Distinct Parsley Extracts.

Arias-Rodríguez, Laura Isabel / Pablos, Jesús L / Vallet-Regí, María / Rodríguez-Mendiola, Martha A / Arias-Castro, Carlos / Sánchez-Salcedo, Sandra / Salinas, Antonio J

Biomolecules

2023 Band 13, Heft 12

Abstract: The increasing interest in innovative solutions for addressing bone defects has driven research into the use of Bioactive Mesoporous Glasses (MBGs). These materials, distinguished by their well-ordered mesoporous structure, possess the capability to ... ...

Abstract	The increasing interest in innovative solutions for addressing bone defects has driven research into the use of Bioactive Mesoporous Glasses (MBGs). These materials, distinguished by their well-ordered mesoporous structure, possess the capability to accommodate plant extracts with well-established osteogenic properties, including bovine lactoferrin (bLF), as part of their 3D scaffold composition. This harmonizes seamlessly with the ongoing advancements in the field of biomedicine. In this study, we fabricated 3D scaffolds utilizing MBGs loaded with extracts from parsley leaves (PL) and embryogenic cultures (EC), rich in bioactive compounds such as apigenin and kaempferol, which hold potential benefits for bone metabolism. Gelatin Methacryloyl (GelMa) served as the polymer, and bLF was included in the formulation. Cytocompatibility, Runx2 gene expression, ALP enzyme activity, and biomineralization were assessed in preosteoblastic MC3T3-E1 cell cultures. MBGs effectively integrated PL and EC extracts with loadings between 22.6 ± 0.1 and 43.6 ± 0.3 µM for PL and 26.3 ± 0.3 and 46.8 ± 0.4 µM for EC, ensuring cell viability through a release percentage between 28.3% and 59.9%. The incorporation of bLF in the 3D scaffold formulation showed significant differences compared to the control in all assays, even at concentrations below 0.2 µM. Combinations, especially PL + bLF at 0.19 µM, demonstrated additive potential, with superior biomineralization compared to EC. In summary, this study highlights the effectiveness of MBGs in incorporating PL and EC extracts, along with bLF, into 3D scaffolds. The results underscore cytocompatibility, osteogenic activity, and biomineralization, offering exciting potential for future in vivo applications.
Mesh-Begriff(e)	Lactoferrin/pharmacology ; Lactoferrin/metabolism ; Petroselinum ; Osteoblasts/metabolism ; Cell Culture Techniques
Chemische Substanzen	gelatin methacryloyl ; Lactoferrin (EC 3.4.21.-)
Sprache	Englisch
Erscheinungsdatum	2023-12-09
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom13121764
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Microglial Caspase-3 is essential for modulating hippocampal neurogenesis.

Alonso Bellido, Isabel M / Posada-Pérez, Mercedes / Hernández-Rasco, Francisco / Vázquez-Reyes, Sandra / Cabanillas, María / Herrera, Antonio J / Bachiller, Sara / Soldán-Hidalgo, Jesús / Espinosa-Oliva, Ana M / Joseph, Bertrand / de Pablos, Rocío M / Venero, José L / Ruiz, Rocío

Brain, behavior, and immunity

2023 Band 112, Seite(n) 206–219

Abstract: Adult hippocampal neurogenesis (AHN) is a process involved in numerous neurodegenerative diseases. Many researchers have described microglia as a key component in regulating the formation and migration of new neurons along the rostral migratory stream. ... ...

Abstract	Adult hippocampal neurogenesis (AHN) is a process involved in numerous neurodegenerative diseases. Many researchers have described microglia as a key component in regulating the formation and migration of new neurons along the rostral migratory stream. Caspase-3 is a cysteine-aspartate-protease classically considered as one of the main effector caspases in the cell death program process. In addition to this classical function, we have identified the role of this protein as a modulator of microglial function; however, its action on neurogenic processes is unknown. The aim of the present study is to identify the role of Caspase-3 in neurogenesis-related microglial functions. To address this study, Caspase-3 conditional knockout mice in the microglia cell line were used. Using this tool, we wanted to elucidate the role of this protein in microglial function in the hippocampus, the main region in which adult neurogenesis takes place. After the reduction of Caspase-3 in microglia, mutant mice showed a reduction of microglia in the hippocampus, especially in the dentate gyrus region, a region inherently associated to neurogenesis. In addition, we found a reduction in doublecortin-positive neurons in conditional Caspase-3 knockout mice, which corresponds to a reduction in neurogenic neurons. Furthermore, using high-resolution image analysis, we also observed a reduction in the phagocytic capacity of microglia lacking Caspase-3. Behavioral analysis using object recognition and Y-maze tests showed altered memory and learning in the absence of Caspase-3. Finally, we identified specific microglia located specifically in neurogenic niche positive for Galectin 3 which colocalized with Cleaved-Caspase-3 in control mice. Taken together, these results showed the essential role of Caspase-3 in microglial function and highlight the relevant role of this specific microglial phenotype in the maintenance of AHN in the hippocampus.
Mesh-Begriff(e)	Animals ; Mice ; Caspase 3/metabolism ; Hippocampus/metabolism ; Mice, Knockout ; Microglia/metabolism ; Neurogenesis/physiology
Chemische Substanzen	Caspase 3 (EC 3.4.22.-)
Sprache	Englisch
Erscheinungsdatum	2023-06-15
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639219-2
ISSN	1090-2139 ; 0889-1591
ISSN (online)	1090-2139
ISSN	0889-1591
DOI	10.1016/j.bbi.2023.06.013
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Partial restoration of gut-mucosal dysbiosis in late-treated HIV-infected subjects with CD4 T-cell recovery.

Olivas-Martínez, Israel / Rosado-Sánchez, Isaac / Cordero-Varela, Juan Antonio / Sobrino, Salvador / Genebat, Miguel / Herrero-Fernández, Inés / de Pablos, Rocío Martínez / Carvajal, Ana Eloísa / Ruiz, Rocío / Isabel Álvarez-Ríos, Ana / Fontillón-Alberdi, María / Bulnes-Ramos, Ángel / Garrido-Rodríguez, Vanesa / Pozo-Balado, María Del Mar / Leal, Manuel / Pacheco, Yolanda María

Clinical and translational medicine

2022 Band 12, Heft 4, Seite(n) e788

Mesh-Begriff(e)	Antiretroviral Therapy, Highly Active ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes ; Dysbiosis ; HIV Infections/complications ; HIV Infections/drug therapy ; Humans
Sprache	Englisch
Erscheinungsdatum	2022-04-19
Erscheinungsland	United States
Dokumenttyp	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2697013-2
ISSN	2001-1326 ; 2001-1326
ISSN (online)	2001-1326
ISSN	2001-1326
DOI	10.1002/ctm2.788
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: A Prospective, Observational Study of the Effect of a High-Calorie, High-Protein Oral Nutritional Supplement with HMB in an Old and Malnourished or at-Risk-of-Malnutrition Population with Hip Fractures: A FracNut Study.

Pareja Sierra, Teresa / Hünicken Torrez, Flavia Lorena / Pablos Hernández, María Carmen / López Velasco, Rosario / Ortés Gómez, Raquel / Cervera Díaz, María Del Carmen / Hormigo Sánchez, Ana Isabel / Perdomo Ramírez, Beatriz / Mora Fernández, Jesús / Jiménez Mola, Sonia / Rodriguez Piñera, María Amparo / Condorhuaman Alvarado, Patricia Ysabel / Sanchez Juan, Carlos / Ramos Clemente, Juan Ignacio / Veses Martín, Silvia / Rodríguez Manzano, Ingrid / González-Colaço Harmand, Magali / Camprubí Robles, María / Martín Aguilar, Andrea /

Saez Lopez, Pilar

Nutrients

2024 Band 16, Heft 8

Abstract: Background: Hip fractures are prevalent among older people, often leading to reduced mobility, muscle loss, and bone density decline. Malnutrition exacerbates the prognosis post surgery. This study aimed to evaluate the impact of a 12-week regimen of a ... ...

Abstract	Background: Hip fractures are prevalent among older people, often leading to reduced mobility, muscle loss, and bone density decline. Malnutrition exacerbates the prognosis post surgery. This study aimed to evaluate the impact of a 12-week regimen of a high-calorie, high-protein oral supplement with β-hydroxy-β-methylbutyrate (HC-HP-HMB-ONS) on nutritional status, daily activities, and compliance in malnourished or at-risk older patients with hip fractures receiving standard care. Subjects and methods: A total of 270 subjects ≥75 years of age, residing at home or in nursing homes, malnourished or at risk of malnutrition, and post hip fracture surgery, received HC-HP-HMB-ONS for 12 weeks. Various scales and questionnaires assessed outcomes. Results: During the 12 weeks of follow-up, 82.8% consumed ≥75% of HC-HP-HMB-ONS. By week 12, 62.4% gained or maintained weight (+0.3 kg), 29.2% achieved normal nutritional status (mean MNA score +2.8), and 46.8% improved nutritional status. Biochemical parameters improved significantly. Subjects reported good tolerability (mean score 8.5/10), with 87.1% of healthcare providers concurring. Conclusions: The administration of HC-HP-HMB-ONS markedly enhanced nutritional status and biochemical parameters in older hip-fracture patients, with high compliance and tolerability. Both patients and healthcare professionals expressed satisfaction with HC-HP-HMB-ONS.
Mesh-Begriff(e)	Humans ; Hip Fractures ; Aged ; Male ; Female ; Dietary Supplements ; Prospective Studies ; Aged, 80 and over ; Malnutrition/etiology ; Nutritional Status ; Valerates/administration & dosage ; Diet, High-Protein ; Administration, Oral ; Energy Intake ; Dietary Proteins/administration & dosage ; Treatment Outcome
Chemische Substanzen	beta-hydroxyisovaleric acid (3F752311CD) ; Valerates ; Dietary Proteins
Sprache	Englisch
Erscheinungsdatum	2024-04-19
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Observational Study
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu16081223
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Pollen Allergens for Molecular Diagnosis.

Pablos, Isabel / Wildner, Sabrina / Asam, Claudia / Wallner, Michael / Gadermaier, Gabriele

Current allergy and asthma reports

2016 Band 16, Heft 4, Seite(n) 31

Abstract: Pollen allergens are one of the main causes of type I allergies affecting up to 30% of the population in industrialized countries. Climatic changes affect the duration and intensity of pollen seasons and may together with pollution contribute to ... ...

Abstract	Pollen allergens are one of the main causes of type I allergies affecting up to 30% of the population in industrialized countries. Climatic changes affect the duration and intensity of pollen seasons and may together with pollution contribute to increased incidences of respiratory allergy and asthma. Allergenic grasses, trees, and weeds often present similar habitats and flowering periods compromising clinical anamnesis. Molecule-based approaches enable distinction between genuine sensitization and clinically mostly irrelevant IgE cross-reactivity due to, e. g., panallergens or carbohydrate determinants. In addition, sensitivity as well as specificity can be improved and lead to identification of the primary sensitizing source which is particularly beneficial regarding polysensitized patients. This review gives an overview on relevant pollen allergens and their usefulness in daily practice. Appropriate allergy diagnosis is directly influencing decisions for therapeutic interventions, and thus, reliable biomarkers are pivotal when considering allergen immunotherapy in the context of precision medicine.
Mesh-Begriff(e)	Allergens/adverse effects ; Desensitization, Immunologic ; Humans ; Molecular Diagnostic Techniques ; Pollen/adverse effects ; Rhinitis, Allergic/diagnosis ; Rhinitis, Allergic/etiology ; Rhinitis, Allergic/therapy
Chemische Substanzen	Allergens
Sprache	Englisch
Erscheinungsdatum	2016-03-28
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2057370-4
ISSN	1534-6315 ; 1529-7322
ISSN (online)	1534-6315
ISSN	1529-7322
DOI	10.1007/s11882-016-0603-z
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Novel antimicrobial agents as alternative to chlorine with potential applications in the fruit and vegetable processing industry

Pablos, Cristina / Aitor Romero / Ana de Diego / Carolina Vargas / Fernando Pérez-Rodríguez / Isabel Bascón / Javier Marugán

International journal of food microbiology. 2018 Nov. 20, v. 285

2018

Abstract: There has been an increasing demand for fresh fruit and vegetables in recent years. Along the processing line in fresh-cut vegetable production, disinfection is one of the most important processing steps affecting the quality and safety, and the shelf- ... ...

Abstract	There has been an increasing demand for fresh fruit and vegetables in recent years. Along the processing line in fresh-cut vegetable production, disinfection is one of the most important processing steps affecting the quality and safety, and the shelf-life of the end produce. Although a range of antimicrobial compounds commonly termed biocides or disinfectants are available, chlorine has long been used to disinfect washing waters of fresh-cut vegetables. However, since chlorine reactions with organic matter lead to the production of by-products, alternative disinfectants to chlorine must be evaluated. A synthetic washing water formula has been developed to determine the antimicrobial efficiency of different families of potential disinfectants: quaternary ammonium compounds (QACs) as benzalkonium chloride (BZK), and didecyldimethylammonium chloride (DDAC); isothiazolinones (mixture of methylchloroisothiazolinone and methylisothiazolinone, CMIT:MIT 3:1 and 1:1); and essential oils (carvacrol, CAR). The twin configuration and higher length of the chains of alkyl groups of DDAC compared to BZK have led to a higher antimicrobial efficiency. In both cases, Gram-positive bacteria seemed to be much more sensitive to the QAC attack than Gram-negative. The opposite happened for CMIT:MIT. The chloro-substituted isothiazolinone (CMIT) has been proven to be much more effective than its unsubstituted form (MIT). In addition, in contrast to chlorine, its antimicrobial activity together with that of DDAC was not decreased when increasing the organic matter content of the water. Synergetic antimicrobial effects have been confirmed when combining BZK and CAR. MBC values were determined in SWW, during 90 s of contact time and Salmonella concentration of 103 CFU/mL, corresponding to: 100 (BZK), 30 (DDAC), 50 (CMIT:MIT 3:1), 100 (CMIT:MIT 1:1), 300 (CAR), 75 (BZK)-200 (CAR), and 9 (free chlorine) mg/L. MBC values for inactivating similar concentration of E. faecalis corresponded to: 50 (BZK), and 10 (DDAC) mg/L. Increasing contact times up to 5 min did not lead to higher antimicrobial efficiencies. CMIT:MIT 3:1 together with DDAC, and combinations of BZK-CAR seem to be a plausible alternative to chlorine.
Schlagwörter	antimicrobial properties ; benzalkonium chloride ; biocides ; byproducts ; carvacrol ; chlorides ; chlorine ; disinfectants ; disinfection ; essential oils ; fresh-cut produce ; fruits ; Gram-positive bacteria ; moieties ; organic matter ; quaternary ammonium compounds ; raw fruit ; Salmonella ; shelf life ; vegetable growing ; vegetable industry ; vegetables ; washing
Sprache	Englisch
Erscheinungsverlauf	2018-1120
Umfang	p. 92-97.
Erscheinungsort	Elsevier B.V.
Dokumenttyp	Artikel
ZDB-ID	87122-9
ISSN	1879-3460 ; 0168-1605
ISSN (online)	1879-3460
ISSN	0168-1605
DOI	10.1016/j.ijfoodmicro.2018.07.029
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: Partial restoration of gut‐mucosal dysbiosis in late‐treated HIV‐infected subjects with CD4 T‐cell recovery

Israel Olivas‐Martínez / Isaac Rosado‐Sánchez / Juan Antonio Cordero‐Varela / Salvador Sobrino / Miguel Genebat / Inés Herrero‐Fernández / Rocío Martínez dePablos / Ana Eloísa Carvajal / Rocío Ruiz / Ana Isabel Álvarez‐Ríos / María Fontillón‐Alberdi / Ángel Bulnes‐Ramos / Vanesa Garrido‐Rodríguez / María del Mar Pozo‐Balado / Manuel Leal / Yolanda María Pacheco

Clinical and Translational Medicine, Vol 12, Iss 4, Pp n/a-n/a (2022)

2022

Schlagwörter	Medicine (General) ; R5-920
Sprache	Englisch
Erscheinungsdatum	2022-04-01T00:00:00Z
Verlag	Wiley
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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