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  1. Artikel ; Online: The immunomodulatory roles of vitamin D: new tricks for an old dog.

    Parravicini, Valentino / Caserta, Stefano

    Molecular interventions

    2010  Band 10, Heft 4, Seite(n) 204–208

    Mesh-Begriff(e) Gene Expression Regulation ; Humans ; Immunologic Factors/physiology ; Models, Biological ; Receptors, Calcitriol/metabolism ; Retinoid X Receptors/metabolism ; Transcription, Genetic ; Vitamin D/immunology
    Chemische Substanzen Immunologic Factors ; Receptors, Calcitriol ; Retinoid X Receptors ; Vitamin D (1406-16-2)
    Sprache Englisch
    Erscheinungsdatum 2010-08
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2108819-6
    ISSN 1543-2548 ; 1534-0384
    ISSN (online) 1543-2548
    ISSN 1534-0384
    DOI 10.1124/mi.10.4.3
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: A novel chemotaxis assay in 3-D collagen gels by time-lapse microscopy.

    Vasaturo, Angela / Caserta, Sergio / Russo, Ilaria / Preziosi, Valentina / Ciacci, Carolina / Guido, Stefano

    PloS one

    2012  Band 7, Heft 12, Seite(n) e52251

    Abstract: The directional cell response to chemical gradients, referred to as chemotaxis, plays an important role in physiological and pathological processes including development, immune response and tumor cell invasion. Despite such implications, chemotaxis ... ...

    Abstract The directional cell response to chemical gradients, referred to as chemotaxis, plays an important role in physiological and pathological processes including development, immune response and tumor cell invasion. Despite such implications, chemotaxis remains a challenging process to study under physiologically-relevant conditions in-vitro, mainly due to difficulties in generating a well characterized and sustained gradient in substrata mimicking the in-vivo environment while allowing dynamic cell imaging. Here, we describe a novel chemotaxis assay in 3D collagen gels, based on a reusable direct-viewing chamber in which a chemoattractant gradient is generated by diffusion through a porous membrane. The diffusion process has been analysed by monitoring the concentration of FITC-labelled dextran through epifluorescence microscopy and by comparing experimental data with theoretical and numerical predictions based on Fick's law. Cell migration towards chemoattractant gradients has been followed by time-lapse microscopy and quantified by cell tracking based on image analysis techniques. The results are expressed in terms of chemotactic index (I) and average cell velocity. The assay has been tested by comparing the migration of human neutrophils in isotropic conditions and in the presence of an Interleukin-8 (IL-8) gradient. In the absence of IL-8 stimulation, 80% of the cells showed a velocity ranging from 0 to 1 µm/min. However, in the presence of an IL-8 gradient, 60% of the cells showed an increase in velocity reaching values between 2 and 7 µm/min. Furthermore, after IL-8 addition, I increased from 0 to 0.25 and 0.25 to 0.5, respectively, for the two donors examined. These data indicate a pronounced directional migration of neutrophils towards the IL-8 gradient in 3D collagen matrix. The chemotaxis assay described here can be adapted to other cell types and may serve as a physiologically relevant method to study the directed locomotion of cells in a 3D environment in response to different chemoattractants.
    Mesh-Begriff(e) Cell Movement/physiology ; Cells, Cultured ; Chemotaxis/physiology ; Collagen ; Humans ; Interleukin-8/metabolism ; Microscopy/methods ; Models, Theoretical
    Chemische Substanzen Interleukin-8 ; Collagen (9007-34-5)
    Sprache Englisch
    Erscheinungsdatum 2012-12-19
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0052251
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Identification of vitamin D receptor as a target of p63.

    Kommagani, R / Caserta, T M / Kadakia, M P

    Oncogene

    2006  Band 25, Heft 26, Seite(n) 3745–3751

    Abstract: ... we provide evidence that p63gamma specifically upregulates vitamin D Receptor (VDR). In contrast, p53 does ...

    Abstract p63, a p53 homolog has been shown to play a role in development and cancer. p63 is essential for both commitment of ectoderm to stratified epithelia and for the proliferative potential of epithelial stem cells. p63 knockout mice are born with severe development defects and lack organs of epithelial origin. In addition, p63 has also been shown to play a role in cancer development through the differential regulation of genes with tumor suppressor function and genes involved in metastasis. In order to understand the role of p63 in cancer and development, genes that are specifically regulated by p63 but not p53 were identified. In this study, we provide evidence that p63gamma specifically upregulates vitamin D Receptor (VDR). In contrast, p53 does not appear to be involved in upregulation of VDR expression. Additionally, we demonstrate that a naturally occurring p63 missense mutant, p63gamma (R279H) and p14(ARF), both act in a dominant negative manner to inhibit p63gamma-mediated upregulation of VDR. Furthermore, using chromatin immunoprecipitation assays, we demonstrated that p63 directly binds to the VDR promoter in vivo. Our findings clearly demonstrate that VDR is a direct target of p63 and suggests that p63 may play a role in cancer and differentiation through modulation of the VDR pathway.
    Mesh-Begriff(e) Bone Neoplasms/genetics ; Bone Neoplasms/pathology ; Cell Differentiation/genetics ; Chromatin Immunoprecipitation ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Gene Expression Regulation ; HeLa Cells ; Humans ; Mutation, Missense ; Osteosarcoma/genetics ; Osteosarcoma/pathology ; Promoter Regions, Genetic ; Receptors, Calcitriol/genetics ; Receptors, Calcitriol/metabolism ; Response Elements ; Trans-Activators/genetics ; Trans-Activators/metabolism ; Transcription Factors ; Tumor Cells, Cultured ; Tumor Suppressor Protein p14ARF/genetics ; Tumor Suppressor Protein p14ARF/metabolism ; Tumor Suppressor Proteins/genetics ; Tumor Suppressor Proteins/metabolism
    Chemische Substanzen DNA-Binding Proteins ; Receptors, Calcitriol ; TP63 protein, human ; Trans-Activators ; Transcription Factors ; Tumor Suppressor Protein p14ARF ; Tumor Suppressor Proteins
    Sprache Englisch
    Erscheinungsdatum 2006-06-22
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 639046-8
    ISSN 1476-5594 ; 0950-9232
    ISSN (online) 1476-5594
    ISSN 0950-9232
    DOI 10.1038/sj.onc.1209412
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: A novel chemotaxis assay in 3-D collagen gels by time-lapse microscopy.

    Angela Vasaturo / Sergio Caserta / Ilaria Russo / Valentina Preziosi / Carolina Ciacci / Stefano Guido

    PLoS ONE, Vol 7, Iss 12, p e

    2012  Band 52251

    Abstract: The directional cell response to chemical gradients, referred to as chemotaxis, plays an important role in physiological and pathological processes including development, immune response and tumor cell invasion. Despite such implications, chemotaxis ... ...

    Abstract The directional cell response to chemical gradients, referred to as chemotaxis, plays an important role in physiological and pathological processes including development, immune response and tumor cell invasion. Despite such implications, chemotaxis remains a challenging process to study under physiologically-relevant conditions in-vitro, mainly due to difficulties in generating a well characterized and sustained gradient in substrata mimicking the in-vivo environment while allowing dynamic cell imaging. Here, we describe a novel chemotaxis assay in 3D collagen gels, based on a reusable direct-viewing chamber in which a chemoattractant gradient is generated by diffusion through a porous membrane. The diffusion process has been analysed by monitoring the concentration of FITC-labelled dextran through epifluorescence microscopy and by comparing experimental data with theoretical and numerical predictions based on Fick's law. Cell migration towards chemoattractant gradients has been followed by time-lapse microscopy and quantified by cell tracking based on image analysis techniques. The results are expressed in terms of chemotactic index (I) and average cell velocity. The assay has been tested by comparing the migration of human neutrophils in isotropic conditions and in the presence of an Interleukin-8 (IL-8) gradient. In the absence of IL-8 stimulation, 80% of the cells showed a velocity ranging from 0 to 1 µm/min. However, in the presence of an IL-8 gradient, 60% of the cells showed an increase in velocity reaching values between 2 and 7 µm/min. Furthermore, after IL-8 addition, I increased from 0 to 0.25 and 0.25 to 0.5, respectively, for the two donors examined. These data indicate a pronounced directional migration of neutrophils towards the IL-8 gradient in 3D collagen matrix. The chemotaxis assay described here can be adapted to other cell types and may serve as a physiologically relevant method to study the directed locomotion of cells in a 3D environment in response to different chemoattractants.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2012-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Efficacy and safety of follitropin delta for ovarian stimulation in vitro fertilization/ intracytoplasmic sperm injection cycles: a systematic review with meta-analysis.

    Palomba, Stefano / Caserta, Donatella / Levi-Setti, Paolo Emanuele / Busnelli, Andrea

    Journal of ovarian research

    2024  Band 17, Heft 1, Seite(n) 60

    Abstract: Background: Follitropin delta is a novel recombinant follicle stimulating hormone preparation uniquely expressed in a human fetal retinal cell line by recombinant DNA technology. To date, no systematic review was available about the safety and the ... ...

    Abstract Background: Follitropin delta is a novel recombinant follicle stimulating hormone preparation uniquely expressed in a human fetal retinal cell line by recombinant DNA technology. To date, no systematic review was available about the safety and the efficacy of the follitropin delta. The objective of this study was systematically reviewing the available literature and to provide updated evidence regarding the efficacy-safety profile of follitropin delta when compared to other gonadotropin formulations for ovarian stimulation in in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles.
    Methods: An extensive search was performed to identify phase 1, phase 2 and phase 3 RCTs in humans focused on follitropin delta use for ovarian stimulation in IVF/ICSI cycles. The risk of bias and the overall quality of the evidence was analyzed. All data were extracted and analyzed using the intention-to-treat principle and expressed per woman randomized.
    Results: A total of 7 RCTs (1 phase 1 RCT, 2 phase 2 RCTs and 4 phase 3 RCTs) were included in the qualitative analysis, whereas data of three phase 3 RCTs were meta-analyzed. All trials compared personalized recombinant follitropin delta treatment versus conventional recombinant follitropin alfa/beta administration in potentially normo-responder patients who receive ovarian stimulation in GnRH antagonist IVF/ICSI cycles. No difference between two regimens was detected for clinical pregnancy rate [odds ratio (OR) 1.06; 95% confidence intervals (CI): 0.90, 1.24; P = 0.49; I
    Conclusion: Personalized follitropin delta treatment is associated with a lower risk of OHSS compared to conventional follitropin alfa/beta administration in potentially normo-responder patients who receive ovarian stimulation in GnRH antagonist IVF/ICSI cycles. The absence of cumulative data does not allow definitive conclusions to be drawn regarding the comparison of the effectiveness of the two treatments.
    Protocol study registration: CRD42023470352 (available at http://www.crd.york.ac.uk/PROSPERO ).
    Mesh-Begriff(e) Pregnancy ; Female ; Humans ; Male ; Sperm Injections, Intracytoplasmic ; Semen ; Fertilization in Vitro/methods ; Ovulation Induction/methods ; Ovarian Hyperstimulation Syndrome/prevention & control ; Follicle Stimulating Hormone ; Gonadotropin-Releasing Hormone ; Recombinant Proteins ; Follicle Stimulating Hormone, Human
    Chemische Substanzen follitropin delta (076WHW89TW) ; Follicle Stimulating Hormone (9002-68-0) ; Gonadotropin-Releasing Hormone (33515-09-2) ; Recombinant Proteins ; Follicle Stimulating Hormone, Human
    Sprache Englisch
    Erscheinungsdatum 2024-03-14
    Erscheinungsland England
    Dokumenttyp Meta-Analysis ; Systematic Review ; Journal Article ; Review
    ZDB-ID 2455679-8
    ISSN 1757-2215 ; 1757-2215
    ISSN (online) 1757-2215
    ISSN 1757-2215
    DOI 10.1186/s13048-024-01372-w
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Exploring the Therapeutic Potential of

    Cancemi, Gabriella / Caserta, Santino / Gangemi, Sebastiano / Pioggia, Giovanni / Allegra, Alessandro

    Journal of clinical medicine

    2024  Band 13, Heft 4

    Abstract: Triterpenoids, such as ganoderic acid, and polysaccharides, including β-D-glucans, α-D-glucans, and ... α-D-mannans, are the main secondary metabolites of the medicinal fungus ...

    Abstract Triterpenoids, such as ganoderic acid, and polysaccharides, including β-D-glucans, α-D-glucans, and α-D-mannans, are the main secondary metabolites of the medicinal fungus
    Sprache Englisch
    Erscheinungsdatum 2024-02-18
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm13041153
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: The role of air relative humidity on the wettability of Pseudomonas fluorescens AR11 biofilms.

    Marra, Daniele / Orillo, Emilia / Toscano, Giuseppe / Petala, Maria / Karapantsios, Thodoris D / Caserta, Sergio

    Colloids and surfaces. B, Biointerfaces

    2024  Band 237, Seite(n) 113831

    Abstract: Biofilms are complex porous materials formed by microorganisms, polysaccharides, proteins, eDNA, inorganic matter, and water. They are ubiquitous in various environmental niches and are known to grow at solid-liquid, solid-air and air-liquid interfaces, ... ...

    Abstract Biofilms are complex porous materials formed by microorganisms, polysaccharides, proteins, eDNA, inorganic matter, and water. They are ubiquitous in various environmental niches and are known to grow at solid-liquid, solid-air and air-liquid interfaces, often causing problems in several industrial and sanitary fields. Their removal is a challenge in many applications and numerous studies have been conducted to identify promising chemical species as cleaning agents. While these substances target specific components of biofilm structure, the role of water content in biofilm, and how it can influence wettability and detergent absorption have been quite neglected in the literature. Estimating water content in biofilm is a challenging task due to its heterogeneity in morphology and chemical composition. In this study, we controlled water content in Pseudomonas fluorescens AR 11 biofilms grown on submerged glass slides by regulating environmental relative humidity after drying. Interfacial properties of biofilm were investigated by measuring wetting of water and soybean oil. The morphology of biofilm structure was evaluated using Confocal Laser Scanning Microscopy and Scanning Electron Microscopy. The results showed that biofilm water content has a significant and measurable effect on its wettability, leading to the hypothesis that a preliminary control of water content can play a crucial role in biofilm removal process.
    Mesh-Begriff(e) Wettability ; Pseudomonas fluorescens/physiology ; Humidity ; Biofilms ; Water
    Chemische Substanzen Water (059QF0KO0R)
    Sprache Englisch
    Erscheinungsdatum 2024-03-02
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2024.113831
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel: Marilyn D. Harris, RN, MSN, CNAA, FAAN.

    Caserta, J E

    Home healthcare nurse

    1992  Band 10, Heft 3, Seite(n) 71–72

    Mesh-Begriff(e) Community Health Nursing/history ; History, 20th Century ; Humans ; Nurse Administrators/history
    Sprache Englisch
    Erscheinungsdatum 1992-05
    Erscheinungsland United States
    Dokumenttyp Biography ; Historical Article ; Journal Article ; Portrait
    ZDB-ID 605757-3
    ISSN 1539-0713 ; 0884-741X
    ISSN (online) 1539-0713
    ISSN 0884-741X
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel: Polycystic ovary syndrome and thyroid disorder: a comprehensive narrative review of the literature.

    Palomba, Stefano / Colombo, Carla / Busnelli, Andrea / Caserta, Donatella / Vitale, Giovanni

    Frontiers in endocrinology

    2023  Band 14, Seite(n) 1251866

    Abstract: ... Lifestyle modification changes, metformin, and vitamin D seem to improve thyroid function ...

    Abstract Background: Published data on the relationship between polycystic ovary syndrome (PCOS) and thyroid dysfunction are sparse and confusing.
    Objective: To comprehensively review data available in the literature regarding the relationship between PCOS and the thyroid function, and its abnormalities.
    Methods: Nine main areas of interest were identified and analyzed according to the available evidence: 1) Evaluation of thyroid function for PCOS diagnosis; 2) Epidemiology data on thyroid function/disorders in patients with PCOS, and vice versa; 3) Experimental data supporting the relationship between thyroid function/disorders and PCOS; 4) Effects of thyroid function/disorders on PCOS features, and vice versa; 5) Effect of thyroid alterations on the cardiometabolic risk in women with PCOS; 6) Effect of thyroid abnormalities on reproductive outcomes in women with PCOS; 7) Relationship between thyroid function/abnormalities in patients with PCOS who are undergoing fertility treatment; 8) Effect of treatments for thyroid diseases on PCOS; and 9) Effect of treatments for PCOS on thyroid function. An extensive literature search for specific keywords was performed for articles published from 1970 to March 2023 using PubMed and Web of Science. Data were reported in a narrative fashion.
    Results: PCOS is a diagnosis of exclusion for which diagnosis is possible only after excluding disorders that mimic the PCOS phenotype, including thyroid dysfunctions. However, the tests and the cutoff values used for this are not specified. Many experimental and clinical data suggest a relationship between perturbations of the thyroid function and PCOS. Direct and unequivocal evidence on the effects of thyroid function/disorders on PCOS features are lacking. High thyroid-stimulating hormone levels and subclinical hypothyroidism may be associated with significant worsening of several intermediate endpoints of cardiometabolic risk in women with PCOS. Thyroid abnormalities may worsen reproductive outcomes, especially in patients undergoing fertility treatment. To date, there are no data demonstrating the efficacy of thyroid medications on fertility and cardiometabolic risk in women with PCOS. Lifestyle modification changes, metformin, and vitamin D seem to improve thyroid function in the general population.
    Conclusion: PCOS and thyroid disorders are closely related, and their coexistence may identify patients with a higher reproductive and metabolic risk. Regular screening for thyroid function and thyroid-specific autoantibodies in women with PCOS, particularly before and during pregnancy, is highly recommended.
    Mesh-Begriff(e) Female ; Humans ; Pregnancy ; Polycystic Ovary Syndrome/complications ; Polycystic Ovary Syndrome/diagnosis ; Thyroid Diseases/complications ; Thyroid Diseases/epidemiology ; Hypothyroidism ; Thyroid Dysgenesis ; Antibodies ; Cardiovascular Diseases
    Chemische Substanzen Antibodies
    Sprache Englisch
    Erscheinungsdatum 2023-08-11
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1251866
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Endometrial Scratching for Improving Endometrial Receptivity: a Critical Review of Old and New Clinical Evidence.

    Palomba, Stefano / Vitagliano, Amerigo / Marci, Roberto / Caserta, Donatella

    Reproductive sciences (Thousand Oaks, Calif.)

    2022  Band 30, Heft 6, Seite(n) 1701–1711

    Abstract: Endometrial scratching is intentional injury of the endometrium intended to improve the reproductive outcomes of infertile women who seek pregnancy. In recent years, several randomized controlled trials, systematic reviews, and meta-analyses of trials ... ...

    Abstract Endometrial scratching is intentional injury of the endometrium intended to improve the reproductive outcomes of infertile women who seek pregnancy. In recent years, several randomized controlled trials, systematic reviews, and meta-analyses of trials have been published, reporting controversial results. Some authors have recommended against endometrial scratching in clinical practice, while others have recommended its application to specific categories of patients. This article aimed to provide a critical analysis of the old and new available evidence, to assist physicians interpreting the published data, and to generate insights for future research on the topic.
    Mesh-Begriff(e) Pregnancy ; Female ; Humans ; Infertility, Female/therapy ; Endometrium ; Reproduction ; Pregnancy Rate
    Sprache Englisch
    Erscheinungsdatum 2022-12-05
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2276411-2
    ISSN 1933-7205 ; 1933-7191
    ISSN (online) 1933-7205
    ISSN 1933-7191
    DOI 10.1007/s43032-022-01125-4
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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