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  1. Artikel: Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

    Cao, Yong-Jun / Li, Han-Zhou / Zhao, Jie / Sun, Yu-Meng / Jin, Xiao-Wen / Lv, Shu-Quan / Luo, Jun-Yu / Fang, Xi-Xing / Wen, Wei-Bo / Liao, Jia-Bao

    Evidence-based complementary and alternative medicine : eCAM

    2022  Band 2022, Seite(n) 2264394

    Abstract: Jian-Gan-Xiao-Zhi decoction (JGXZ) has demonstrated beneficial effects on nonalcoholic fatty liver ...

    Abstract Jian-Gan-Xiao-Zhi decoction (JGXZ) has demonstrated beneficial effects on nonalcoholic fatty liver disease (NAFLD). However, the mechanisms by which JGXZ improve NAFLD are still unclear.
    Sprache Englisch
    Erscheinungsdatum 2022-07-08
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2022/2264394
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Jian-Gan-Xiao-Zhi Decoction Alleviates Inflammatory Response in Nonalcoholic Fatty Liver Disease Model Rats through Modulating Gut Microbiota.

    Liao, Jiabao / Xie, Xuehua / Gao, Jinmei / Zhang, Zhaiyi / Qu, Fei / Cui, Huantian / Cao, Yongjun / Han, Xue / Zhao, Jie / Wen, Weibo / Wang, Hongwu

    Evidence-based complementary and alternative medicine : eCAM

    2021  Band 2021, Seite(n) 5522755

    Abstract: Background: Jian-Gan-Xiao-Zhi decoction (JGXZ), composed of : Methods: In this study, a NAFLD ...

    Abstract Background: Jian-Gan-Xiao-Zhi decoction (JGXZ), composed of
    Methods: In this study, a NAFLD rat model induced by a high-fat diet (HFD) received oral treatment of JGXZ (8 or 16 g crude herb/kg) for 12 weeks. The therapeutic effects of JGXZ on NAFLD model rats were investigated through blood lipid levels and pathological liver changes. 16S rRNA analysis was used to study the changes in gut microbiota after JGXZ treatment. The expressions of occludin and tight junction protein 1 (ZO-1) in the colon were investigated using immunostaining to study the effects of JGXZ on gut permeability. The anti-inflammatory effects of JGXZ were also studied through measuring the levels of IL-1
    Results: JGXZ treatment could decrease body weight and ameliorate dyslipidemia in NAFLD model rats. H&E and Oil Red O staining indicated that JGXZ reduced steatosis and infiltration of inflammatory cells in the liver. 16S rRNA analysis showed that JGXZ impacted the diversity of gut microbiota, decreasing the
    Conclusions: Our study illustrated that JGXZ could ameliorate NAFLD through modulating gut microbiota, decreasing gut permeability, and alleviating inflammatory response.
    Sprache Englisch
    Erscheinungsdatum 2021-03-20
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2021/5522755
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  3. Artikel ; Online: Jian-Gan-Xiao-Zhi Decoction Alleviates Inflammatory Response in Nonalcoholic Fatty Liver Disease Model Rats through Modulating Gut Microbiota

    Jiabao Liao / Xuehua Xie / Jinmei Gao / Zhaiyi Zhang / Fei Qu / Huantian Cui / Yongjun Cao / Xue Han / Jie Zhao / Weibo Wen / Hongwu Wang

    Evidence-Based Complementary and Alternative Medicine, Vol

    2021  Band 2021

    Abstract: Background. Jian-Gan-Xiao-Zhi decoction (JGXZ), composed of Salvia miltiorrhiza Bunge ...

    Abstract Background. Jian-Gan-Xiao-Zhi decoction (JGXZ), composed of Salvia miltiorrhiza Bunge, Panax notoginseng, Curcuma zedoaria, and other 9 types of herbs, has demonstrated beneficial effects on nonalcoholic fatty liver disease (NAFLD). However, the mechanisms behind JGXZ’s impact on NAFLD remain unknown. Methods. In this study, a NAFLD rat model induced by a high-fat diet (HFD) received oral treatment of JGXZ (8 or 16 g crude herb/kg) for 12 weeks. The therapeutic effects of JGXZ on NAFLD model rats were investigated through blood lipid levels and pathological liver changes. 16S rRNA analysis was used to study the changes in gut microbiota after JGXZ treatment. The expressions of occludin and tight junction protein 1 (ZO-1) in the colon were investigated using immunostaining to study the effects of JGXZ on gut permeability. The anti-inflammatory effects of JGXZ were also studied through measuring the levels of IL-1β, IL-6, and TNF-α in the serum and liver. Results. JGXZ treatment could decrease body weight and ameliorate dyslipidemia in NAFLD model rats. H&E and Oil Red O staining indicated that JGXZ reduced steatosis and infiltration of inflammatory cells in the liver. 16S rRNA analysis showed that JGXZ impacted the diversity of gut microbiota, decreasing the Firmicutes–to-Bacteroidetes ratio, and increasing the relative abundance of probiotics, such as Alloprevotella, Lactobacillus, and Turicibacter. Gut permeability evaluation found that the expressions of ZO-1 and occludin in the colon were increased after JGXZ treatment. Moreover, JGXZ treatment could decrease the levels of IL-1β, IL-6, and TNF-α in the serum and liver. Conclusions. Our study illustrated that JGXZ could ameliorate NAFLD through modulating gut microbiota, decreasing gut permeability, and alleviating inflammatory response.
    Schlagwörter Other systems of medicine ; RZ201-999
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: PFKP deubiquitination and stabilization by USP5 activate aerobic glycolysis to promote triple-negative breast cancer progression.

    Peng, Zi-Mei / Han, Xiao-Jian / Wang, Tao / Li, Jian-Jun / Yang, Chun-Xi / Tou, Fang-Fang / Zhang, Zhen

    Breast cancer research : BCR

    2024  Band 26, Heft 1, Seite(n) 10

    Abstract: Background: Triple-negative breast cancer (TNBC) remains the most challenging subtype of breast cancer and lacks definite treatment targets. Aerobic glycolysis is a hallmark of metabolic reprogramming that contributes to cancer progression. PFKP is a ... ...

    Abstract Background: Triple-negative breast cancer (TNBC) remains the most challenging subtype of breast cancer and lacks definite treatment targets. Aerobic glycolysis is a hallmark of metabolic reprogramming that contributes to cancer progression. PFKP is a rate-limiting enzyme involved in aerobic glycolysis, which is overexpressed in various types of cancers. However, the underlying mechanisms and roles of the posttranslational modification of PFKP in TNBC remain unknown.
    Methods: To explore whether PFKP protein has a potential role in the progression of TNBC, protein levels of PFKP in TNBC and normal breast tissues were examined by CPTAC database analysis, immunohistochemistry staining (IHC), and western blotting assay. Further CCK-8 assay, colony formation assay, EDU incorporation assay, and tumor xenograft experiments were used to detect the effect of PFKP on TNBC progression. To clarify the role of the USP5-PFKP pathway in TNBC progression, ubiquitin assay, co-immunoprecipitation (Co-IP), mass spectrometry-based protein identification, western blotting assay, immunofluorescence microscopy, in vitro binding assay, and glycolysis assay were conducted.
    Results: Herein, we showed that PFKP protein was highly expressed in TNBC, which was associated with TNBC progression and poor prognosis of patients. In addition, we demonstrated that PFKP depletion significantly inhibited the TNBC progression in vitro and in vivo. Importantly, we identified that PFKP was a bona fide target of deubiquitinase USP5, and the USP5-mediated deubiquitination and stabilization of PFKP were essential for cancer cell aerobic glycolysis and TNBC progression. Moreover, we found a strong positive correlation between the expression of USP5 and PFKP in TNBC samples. Notably, the high expression of USP5 and PFKP was significantly correlated with poor clinical outcomes.
    Conclusions: Our study established the USP5-PFKP axis as an important regulatory mechanism of TNBC progression and provided a rationale for future therapeutic interventions in the treatment of TNBC.
    Mesh-Begriff(e) Humans ; Cell Line, Tumor ; Cell Proliferation ; Glycolysis ; Heterografts ; Transplantation, Heterologous ; Triple Negative Breast Neoplasms/pathology
    Chemische Substanzen ubiquitin isopeptidase (EC 3.4.99.-) ; PFKP protein, human (EC 2.7.1.11)
    Sprache Englisch
    Erscheinungsdatum 2024-01-12
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2015059-3
    ISSN 1465-542X ; 1465-5411
    ISSN (online) 1465-542X
    ISSN 1465-5411
    DOI 10.1186/s13058-024-01767-z
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  5. Artikel ; Online: Natural Products in Liver Fibrosis Management: A Five-year Review.

    Wang, Tao / Lu, Zhuo / Sun, Gui-Feng / He, Kai-Yi / Chen, Zhi-Ping / Qu, Xin-Hui / Han, Xiao-Jian

    Current medicinal chemistry

    2024  

    Abstract: Liver fibrosis, characterized by the overproduction of extracellular matrix proteins within liver tissue, poses a rising global health concern. However, no approved antifibrotic drugs are currently available, highlighting the critical need for ... ...

    Abstract Liver fibrosis, characterized by the overproduction of extracellular matrix proteins within liver tissue, poses a rising global health concern. However, no approved antifibrotic drugs are currently available, highlighting the critical need for understanding the molecular mechanisms of liver fibrosis. This knowledge could not only aid in developing therapies but also enable early intervention, enhance disease prediction, and improve our understanding of the interaction between various underlying conditions and the liver. Notably, natural products used in traditional medicine systems worldwide and demonstrating diverse biochemical and pharmacological activities are increasingly recognized for their potential in treating liver fibrosis. This review aims to comprehensively understand liver fibrosis, emphasizing the molecular mechanisms and advancements in exploring natural products' antifibrotic potential over the past five years. It also acknowledges the challenges in their development and seeks to underscore their potency in enhancing patient prognosis and reducing the global burden of liver disease.
    Sprache Englisch
    Erscheinungsdatum 2024-02-14
    Erscheinungsland United Arab Emirates
    Dokumenttyp Journal Article
    ZDB-ID 1319315-6
    ISSN 1875-533X ; 0929-8673
    ISSN (online) 1875-533X
    ISSN 0929-8673
    DOI 10.2174/0109298673288458240203064112
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: The mechanisms of natural products for eye disorders by targeting mitochondrial dysfunction.

    Sun, Gui-Feng / Qu, Xin-Hui / Jiang, Li-Ping / Chen, Zhi-Ping / Wang, Tao / Han, Xiao-Jian

    Frontiers in pharmacology

    2024  Band 15, Seite(n) 1270073

    Abstract: The human eye is susceptible to various disorders that affect its structure or function, including glaucoma, age-related macular degeneration (AMD) and diabetic retinopathy (DR). Mitochondrial dysfunction has been identified as a critical factor in the ... ...

    Abstract The human eye is susceptible to various disorders that affect its structure or function, including glaucoma, age-related macular degeneration (AMD) and diabetic retinopathy (DR). Mitochondrial dysfunction has been identified as a critical factor in the pathogenesis and progression of eye disorders, making it a potential therapeutic target in the clinic. Natural products have been used in traditional medicine for centuries and continue to play a significant role in modern drug development and clinical therapeutics. Recently, there has been a surge in research exploring the efficacy of natural products in treating eye disorders and their underlying physiological mechanisms. This review aims to discuss the involvement of mitochondrial dysfunction in eye disorders and summarize the recent advances in the application of natural products targeting mitochondria. In addition, we describe the future perspective and challenges in the development of mitochondria-targeting natural products.
    Sprache Englisch
    Erscheinungsdatum 2024-04-25
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2024.1270073
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  7. Artikel ; Online: Human amniotic mesenchymal stem cells-derived conditioned medium and exosomes alleviate oxidative stress-induced retinal degeneration by activating PI3K/Akt/FoxO3 pathway.

    Peng, Zhe-Qing / Guan, Xiao-Hui / Yu, Zhen-Ping / Wu, Jie / Han, Xin-Hao / Li, Ming-Hui / Qu, Xin-Hui / Chen, Zhi-Ping / Han, Xiao-Jian / Wang, Xiao-Yu

    Experimental eye research

    2024  Band 244, Seite(n) 109919

    Abstract: Age-related macular degeneration (AMD) is the leading cause of vision loss among the elderly, which is primarily attributed to oxidative stress-induced damage to the retinal pigment epithelium (RPE). Human amniotic mesenchymal stem cells (hAMSC) were ... ...

    Abstract Age-related macular degeneration (AMD) is the leading cause of vision loss among the elderly, which is primarily attributed to oxidative stress-induced damage to the retinal pigment epithelium (RPE). Human amniotic mesenchymal stem cells (hAMSC) were considered to be one of the most promising stem cells for clinical application due to their low immunogenicity, tissue repair ability, pluripotent potential and potent paracrine effects. The conditional medium (hAMSC-CM) and exosomes (hAMSC-exo) derived from hAMSC, as mediators of intercellular communication, play an important role in the treatment of retinal diseases, but their effect and mechanism on oxidative stress-induced retinal degeneration are not explored. Here, we reported that hAMSC-CM alleviated H
    Sprache Englisch
    Erscheinungsdatum 2024-05-08
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 80122-7
    ISSN 1096-0007 ; 0014-4835
    ISSN (online) 1096-0007
    ISSN 0014-4835
    DOI 10.1016/j.exer.2024.109919
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  8. Artikel ; Online: [Determination of nine aromatic amines in water by cloud point extraction-gas chromatography-mass spectrometry].

    Yang, Chao / Liu, Jing-Long / Xu, Xiao-Jian / Wu, Li-Juan / Yin, Ming-Ming / Dai, Wei / Han, Qian

    Se pu = Chinese journal of chromatography

    2024  Band 42, Heft 3, Seite(n) 296–303

    Abstract: Aromatic amines are a class of compounds bearing amino groups on their benzene rings; these compounds are important raw materials for the industrial production of rubber chemicals, pesticides, dyes, pharmaceuticals, photosensitive chemicals, and ... ...

    Abstract Aromatic amines are a class of compounds bearing amino groups on their benzene rings; these compounds are important raw materials for the industrial production of rubber chemicals, pesticides, dyes, pharmaceuticals, photosensitive chemicals, and agricultural chemicals. Research has revealed that some aromatic amines teratogenetic, carcinogenic, and mutagenic properties. Given the high toxicity and potential harm caused by aromatic amines, monitoring their levels in water sources is critical. Aromatic amines are among the 14 strategic environmental pollutants blacklisted in China, and assessing their exposure levels is essential for protecting human health and the environment. At present, the standard method for detecting aromatic amines in water is liquid-liquid extraction-gas chromatography-mass spectrometry (LLE-GC-MS). However, this method has the disadvantages of large sample size requirement, complex operation, long analysis time, and high reagent consumption. In this study, instead of traditional LLE technology, cloud point extraction (CPE) technology was used in combination with GC-MS to establish an efficient, sensitive, and environment-friendly method for the detection of nine aromatic amines, namely, 2-chloramine, 3-chloramine, 4-chloramine, 2-nitroaniline, 3-nitroaniline, 4-nitroaniline, 1-naphthylamine, 2-naphthylamine, and 4-aminobenzene, in water. Triton X-114 was used as the extraction agent. The main experimental parameters were optimized using a single-factor optimization method. The aromatic amines in various water samples were quantitatively analyzed using GC-MS. The nine aromatic amines were separated on a DB-35 MS capillary column (30 m×0.25 mm×0.25 μm). The mass spectrometer was operated in selected ion monitoring (SIM) mode, and quantitative analysis was performed using the internal standard method. The results demonstrated that all nine aromatic amines could be completely separated within 16 min and had good linearities within accurate mass concentration ranges, with correlation coefficients (
    Sprache Chinesisch
    Erscheinungsdatum 2024-03-18
    Erscheinungsland China
    Dokumenttyp English Abstract ; Journal Article
    ISSN 1872-2059
    ISSN (online) 1872-2059
    DOI 10.3724/SP.J.1123.2023.06002
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  9. Artikel ; Online: [Determination of phenoxyacetic herbicides, metabolites of organophosphorus and pyrethroid pesticides in human urine using solid phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry].

    Zhang, Xu / Han, Lin-Xue / Qiu, Tian / Hu, Xiao-Jian / Zhu, Ying / Yang, Yan-Wei

    Se pu = Chinese journal of chromatography

    2023  Band 41, Heft 3, Seite(n) 224–232

    Abstract: Pesticides are widely used in most agricultural areas to protect food crops but adversely affect ecosystems and human beings. Pesticides have attracted great public concern due to their toxic properties and ubiquitous occurrence in the environment. China ...

    Abstract Pesticides are widely used in most agricultural areas to protect food crops but adversely affect ecosystems and human beings. Pesticides have attracted great public concern due to their toxic properties and ubiquitous occurrence in the environment. China is one of the largest users and producers of pesticides globally. However, limited data are available on pesticide exposure in humans, which warrants a method for quantification of pesticides in human samples. In the present study, we validated and developed a comprehensive and sensitive method for the quantification of two phenoxyacetic herbicides, two metabolites of organophosphorus pesticides and four metabolites of pyrethroid pesticides in human urine using 96-well plate solid phase extraction (SPE) coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For this purpose, a systematic optimization of the chromatographic separation conditions and MS/MS parameters was conducted. Six solvents were optimized for the extraction and clean-up of human urine samples. The targeted compounds in the human urine samples were well separated within 16 min in one analytical run. A 1 mL aliquot of human urine sample was mixed with 0.5 mL sodium acetate buffer (0.2 mol/L) and hydrolyzed by
    Mesh-Begriff(e) Humans ; Pesticides ; Herbicides ; Pyrethrins ; Tandem Mass Spectrometry ; Chromatography, Liquid ; Ecosystem ; Organophosphorus Compounds ; Benzoic Acid ; 2,4,5-Trichlorophenoxyacetic Acid ; 2,4-Dichlorophenoxyacetic Acid
    Chemische Substanzen Pesticides ; Herbicides ; Pyrethrins ; 3-phenoxybenzoic acid (69DC2655VH) ; Organophosphorus Compounds ; Benzoic Acid (8SKN0B0MIM) ; 2,4,5-Trichlorophenoxyacetic Acid (9Q963S4YMX) ; 2,4-Dichlorophenoxyacetic Acid (2577AQ9262)
    Sprache Chinesisch
    Erscheinungsdatum 2023-03-01
    Erscheinungsland China
    Dokumenttyp English Abstract ; Journal Article
    ISSN 1872-2059
    ISSN (online) 1872-2059
    DOI 10.3724/SP.J.1123.2022.05005
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  10. Artikel ; Online: Giant Hepatic Hemangioma.

    Feng, Zi-Han / Chen, Xiao-Feng / Jiang, Jian-Shuai

    Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association

    2024  

    Sprache Englisch
    Erscheinungsdatum 2024-05-07
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2119789-1
    ISSN 1542-7714 ; 1542-3565
    ISSN (online) 1542-7714
    ISSN 1542-3565
    DOI 10.1016/j.cgh.2024.04.024
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