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  1. Artikel ; Online: Bringing to Light the Importance of the miRNA Methylome in Colorectal Cancer Prognosis Through Electrochemical Bioplatforms.

    Povedano, Eloy / Ruiz-Valdepeñas Montiel, Víctor / Sebuyoya, Ravery / Torrente-Rodríguez, Rebeca M / Garranzo-Asensio, Maria / Montero-Calle, Ana / Pingarrón, José M / Barderas, Rodrigo / Bartosik, Martin / Campuzano, Susana

    Analytical chemistry

    2024  Band 96, Heft 11, Seite(n) 4580–4588

    Abstract: This work reports the first electrochemical bioplatforms developed for the determination of the total contents of either target miRNA or methylated target miRNA. The bioplatforms are based on the hybridization of the target miRNA with a synthetic ... ...

    Abstract This work reports the first electrochemical bioplatforms developed for the determination of the total contents of either target miRNA or methylated target miRNA. The bioplatforms are based on the hybridization of the target miRNA with a synthetic biotinylated DNA probe, the capture of the formed DNA/miRNA heterohybrids on the surface of magnetic microcarriers, and their recognition with an antibody selective to these heterohybrids or to the
    Mesh-Begriff(e) Humans ; MicroRNAs/genetics ; MicroRNAs/analysis ; Epigenome ; Nucleic Acid Hybridization/methods ; Antibodies/genetics ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/genetics ; Prognosis ; Biosensing Techniques/methods
    Chemische Substanzen MicroRNAs ; Antibodies
    Sprache Englisch
    Erscheinungsdatum 2024-02-13
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c05474
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Dextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality.

    Gao, Shipeng / Torrente-Rodríguez, Rebeca M / Pedrero, María / Pingarrón, José M / Campuzano, Susana / Rocha-Martin, Javier / Guisán, José M

    Talanta

    2022  Band 247, Seite(n) 123549

    Abstract: Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional ... ...

    Abstract Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional conformation and high biofunctionality retention of attached antibodies. In this work, peroxidase (HRP) was selected as model target analyte, and stable antibody-MNP conjugates were prepared using polyaldehyde-dextrans as multivalent linkers, also to prevent nanoparticles agglomeration and steric shielding of non-specific proteins. Under the manipulation of the oxidation variables, MNP-conjugated antibody showed the highest Fab accessibility, of 1.32 μmol analyte per μmol antibody, corresponding to 139 μmol aldehyde per gram of nanocarrier (5 mM NaIO
    Mesh-Begriff(e) Antibodies ; Biosensing Techniques/methods ; Dextrans/chemistry ; Immunoassay/methods ; Magnetics ; Magnetite Nanoparticles/chemistry ; Nanoparticles/chemistry
    Chemische Substanzen Antibodies ; Dextrans ; Magnetite Nanoparticles
    Sprache Englisch
    Erscheinungsdatum 2022-05-16
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2022.123549
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  3. Artikel ; Online: Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion.

    Quinchia, Jennifer / Blázquez-García, Marina / Torrente-Rodríguez, Rebeca M / Ruiz-Valdepeñas Montiel, Víctor / Serafín, Verónica / Rejas-González, Raquel / Montero-Calle, Ana / Orozco, Jahir / Pingarrón, José M / Barderas, Rodrigo / Campuzano, Susana

    Talanta

    2023  Band 267, Seite(n) 125155

    Abstract: Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin ... ...

    Abstract Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H
    Mesh-Begriff(e) Humans ; Hydrogen Peroxide/chemistry ; Antibodies/chemistry ; Immunoconjugates/chemistry ; Magnetics ; Horseradish Peroxidase ; Glycoproteins ; Biosensing Techniques/methods ; Electrochemical Techniques/methods ; Electrodes ; Immunoassay/methods ; Limit of Detection ; Neoplasms
    Chemische Substanzen Hydrogen Peroxide (BBX060AN9V) ; Antibodies ; Immunoconjugates ; Horseradish Peroxidase (EC 1.11.1.-) ; Glycoproteins
    Sprache Englisch
    Erscheinungsdatum 2023-09-04
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2023.125155
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  4. Artikel: p53 and p63 Proteoforms Derived from Alternative Splicing Possess Differential Seroreactivity in Colorectal Cancer with Distinct Diagnostic Ability from the Canonical Proteins.

    Montero-Calle, Ana / Garranzo-Asensio, María / Torrente-Rodríguez, Rebeca M / Ruiz-Valdepeñas Montiel, Víctor / Poves, Carmen / Dziaková, Jana / Sanz, Rodrigo / Díaz Del Arco, Cristina / Pingarrón, José Manuel / Fernández-Aceñero, María Jesús / Campuzano, Susana / Barderas, Rodrigo

    Cancers

    2023  Band 15, Heft 7

    Abstract: Colorectal cancer (CRC) is the third most common cancer and the second most frequent cause of cancer-related death worldwide. The detection in plasma samples of autoantibodies against specific tumor-associated antigens has been demonstrated to be useful ... ...

    Abstract Colorectal cancer (CRC) is the third most common cancer and the second most frequent cause of cancer-related death worldwide. The detection in plasma samples of autoantibodies against specific tumor-associated antigens has been demonstrated to be useful for the early diagnosis of CRC by liquid biopsy. However, new studies related to the humoral immune response in cancer are needed to enable blood-based diagnosis of the disease. Here, our aim was to characterize the humoral immune response associated with the different p53 and p63 proteoforms derived from alternative splicing and previously described as aberrantly expressed in CRC. Thus, here we investigated the diagnostic ability of the twelve p53 proteoforms and the eight p63 proteoforms described to date, and their specific N-terminal and C-terminal end peptides, by means of luminescence HaloTag beads immunoassays. Full-length proteoforms or specific peptides were cloned as HaloTag fusion proteins and their seroreactivity analyzed using plasma from CRC patients at stages I-IV (
    Sprache Englisch
    Erscheinungsdatum 2023-03-31
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers15072102
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring.

    Torrente-Rodríguez, Rebeca M / Lukas, Heather / Tu, Jiaobing / Min, Jihong / Yang, Yiran / Xu, Changhao / Rossiter, Harry B / Gao, Wei

    Matter

    2020  Band 3, Heft 6, Seite(n) 1981–1998

    Abstract: The COVID-19 pandemic is an ongoing global challenge for public health systems. Ultrasensitive and early identification of infection is critical in preventing widespread COVID-19 infection by presymptomatic and asymptomatic individuals, especially in the ...

    Abstract The COVID-19 pandemic is an ongoing global challenge for public health systems. Ultrasensitive and early identification of infection is critical in preventing widespread COVID-19 infection by presymptomatic and asymptomatic individuals, especially in the community and in-home settings. We demonstrate a multiplexed, portable, wireless electrochemical platform for ultra-rapid detection of COVID-19: the SARS-CoV-2 RapidPlex. It detects viral antigen nucleocapsid protein, IgM and IgG antibodies, as well as the inflammatory biomarker C-reactive protein, based on our mass-producible laser-engraved graphene electrodes. We demonstrate ultrasensitive, highly selective, and rapid electrochemical detection in the physiologically relevant ranges. We successfully evaluated the applicability of our SARS-CoV-2 RapidPlex platform with COVID-19-positive and COVID-19-negative blood and saliva samples. Based on this pilot study, our multiplexed immunosensor platform may allow for high-frequency at-home testing for COVID-19 telemedicine diagnosis and monitoring.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-10-05
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2590-2385
    ISSN (online) 2590-2385
    DOI 10.1016/j.matt.2020.09.027
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  6. Artikel: Dextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality

    Gao, Shipeng / Torrente-Rodríguez, Rebeca M. / Pedrero, María / Pingarrón, José M. / Campuzano, Susana / Rocha-Martin, Javier / Guisán, José M.

    Talanta. 2022 Sept. 01, v. 247

    2022  

    Abstract: Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional ... ...

    Abstract Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional conformation and high biofunctionality retention of attached antibodies. In this work, peroxidase (HRP) was selected as model target analyte, and stable antibody-MNP conjugates were prepared using polyaldehyde-dextrans as multivalent linkers, also to prevent nanoparticles agglomeration and steric shielding of non-specific proteins. Under the manipulation of the oxidation variables, MNP-conjugated antibody showed the highest Fab accessibility, of 1.32 μmol analyte per μmol antibody, corresponding to 139 μmol aldehyde per gram of nanocarrier (5 mM NaIO₄, 4 h). Demonstrating anti-interference advantage up to 10% serum, colorimetric immunoassay gave a detection limit (LOD) of 300 ng mL⁻¹, while electrochemical transduction led to a considerable (680 times) improvement, with a LOD of 0.44 ng mL⁻¹. In addition, polyaldehyde-dextran showed priority over polycarboxylated-dextran as the multivalent antibody crosslinker for MNPs in terms of sensitivity and LOD value, while immunosensors constructed with carboxylated magnetic microbeads (HOOC-MBs) outperformed MNPs-based immunoplatforms. This work sheds light on the importance of surface chemistry (type and density of functional groups) and the dimension (nanosize vs micrometer) of magnetic carriers to conjugate antibodies with better directional orientation and improve the analytical performance of the resulting immunosensors.
    Schlagwörter aldehydes ; antibodies ; blood serum ; carboxylation ; colorimetry ; detection limit ; electrochemistry ; immunoassays ; immunosensors ; magnetism ; microbeads ; nanocarriers ; oxidation ; peroxidase
    Sprache Englisch
    Erscheinungsverlauf 2022-0901
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2022.123549
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  7. Artikel: Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors.

    Torrente-Rodríguez, Rebeca M / Montero-Calle, Ana / San Bartolomé, Clara / Cano, Olga / Vázquez, Mónica / Iglesias-Caballero, María / Corral-Lugo, Andrés / McConnell, Michael J / Pascal, Mariona / Mas, Vicente / Pingarrón, José M / Barderas, Rodrigo / Campuzano, Susana

    Angewandte Chemie (Weinheim an der Bergstrasse, Germany)

    2022  Band 134, Heft 28, Seite(n) e202203662

    Abstract: The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house ... ...

    Abstract The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H
    Sprache Englisch
    Erscheinungsdatum 2022-05-24
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 506609-8
    ISSN 1521-3757 ; 0044-8249 ; 0932-2140
    ISSN (online) 1521-3757
    ISSN 0044-8249 ; 0932-2140
    DOI 10.1002/ange.202203662
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  8. Artikel ; Online: Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors.

    Torrente-Rodríguez, Rebeca M / Montero-Calle, Ana / San Bartolomé, Clara / Cano, Olga / Vázquez, Mónica / Iglesias-Caballero, María / Corral-Lugo, Andrés / McConnell, Michael J / Pascal, Mariona / Mas, Vicente / Pingarrón, José M / Barderas, Rodrigo / Campuzano, Susana

    Angewandte Chemie (International ed. in English)

    2022  Band 61, Heft 28, Seite(n) e202203662

    Abstract: The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house ... ...

    Abstract The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H
    Mesh-Begriff(e) Antibodies, Viral ; Biosensing Techniques ; COVID-19/diagnosis ; COVID-19 Testing ; Humans ; Immunity ; Immunoglobulin G ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
    Chemische Substanzen Antibodies, Viral ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Sprache Englisch
    Erscheinungsdatum 2022-05-24
    Erscheinungsland Germany
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202203662
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  9. Artikel ; Online: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases.

    Torrente-Rodríguez, Rebeca M / Martín, Cristina Muñoz-San / Gamella, Maria / Pedrero, María / Martínez-Bosch, Neus / Navarro, Pilar / García de Frutos, Pablo / Pingarrón, José M / Campuzano, Susana

    Biosensors

    2021  Band 11, Heft 6

    Abstract: A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). ... ...

    Abstract A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL
    Mesh-Begriff(e) Antibodies ; Biosensing Techniques ; Carbon ; Electrochemical Techniques ; Electrodes ; Enzyme-Linked Immunosorbent Assay ; Humans ; Hydrogen Peroxide ; Immunoassay ; Limit of Detection ; Magnetics ; Neoplasms/diagnosis
    Chemische Substanzen Antibodies ; Carbon (7440-44-0) ; Hydrogen Peroxide (BBX060AN9V)
    Sprache Englisch
    Erscheinungsdatum 2021-06-21
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios11060202
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: Magnetic microbeads-based amperometric immunoplatform for the rapid and sensitive detection of N6-methyladenosine to assist in metastatic cancer cells discrimination

    Povedano, Eloy / Gamella, María / Torrente-Rodríguez, Rebeca M / Montero-Calle, Ana / Pedrero, María / Solís-Fernández, Guillermo / Navarro-Villoslada, Fernando / Barderas, Rodrigo / Campuzano, Susana / Pingarrón, José M

    Biosensors & bioelectronics. 2021 Jan. 01, v. 171

    2021  

    Abstract: This work describes the preparation of an immunoplatform for the sensitive and selective determination of N6-methyladenosine (m6A). The simple and fast protocol involves for the first time the use of micromagnetic immunoconjugates to establish a direct ... ...

    Abstract This work describes the preparation of an immunoplatform for the sensitive and selective determination of N6-methyladenosine (m6A). The simple and fast protocol involves for the first time the use of micromagnetic immunoconjugates to establish a direct competitive assay between the m6A target and a biotinylated RNA oligomer bearing a single m6A enzymatically labelled with a commercial conjugate of streptavidin-peroxidase (Strep-HRP) as tracer. The cathodic current change measured in the presence of H₂O₂/hydroquinone (HQ) at screen-printed carbon electrodes (SPCEs) upon surface capturing the magnetic bioconjugates is inversely proportional to the m6A target concentration. After evaluating the effect of key variables, the analytical characteristics were established for the determination of three different targets: the N6-methyladenosine-5′-triphosphate (m6ATP) ribonucleotide, a short synthetic RNA oligomer bearing a single m6A and the positive control provided in a commercial colorimetric kit for m6A-RNA quantification. The obtained results show that this immunoplatform is competitive with other methods reported to date, achieving an improved sensitivity (limit of detection of 0.9 pM for the short synthetic oligomer) using a much simpler and faster protocol (~1 h) and disposable electrodes for the transduction. Furthermore, the applicability for discriminating the metastatic potential of cancer cells by directly analyzing a small amount of raw total RNA without enriching or fragmenting was also preliminary assessed.
    Schlagwörter RNA ; biosensors ; carbon ; colorimetry ; detection limit ; magnetism ; metastasis
    Sprache Englisch
    Erscheinungsverlauf 2021-0101
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2020.112708
    Datenquelle NAL Katalog (AGRICOLA)

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