LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Erweiterte Suche

Suchergebnis

Treffer 1 - 3 von insgesamt 3

Suchoptionen

  1. Artikel: Visualisation of ribosomes in

    Singh, Anand K / Abdullahi, Akilu / Soller, Matthias / David, Alexandre / Brogna, Saverio

    Biology open

    2020  Band 8, Heft 12

    Abstract: The distribution of assembled, and potentially translating, ribosomes within cells can be visualised ... ...

    Abstract The distribution of assembled, and potentially translating, ribosomes within cells can be visualised in
    Mesh-Begriff(e) Animals ; Axons/metabolism ; Drosophila/genetics ; Drosophila/metabolism ; Fluorescent Antibody Technique ; Humans ; Molecular Imaging/methods ; Molecular Structure ; Neurons/metabolism ; Photoreceptor Cells/metabolism ; Ribosomal Proteins/metabolism ; Ribosomes/chemistry ; Ribosomes/metabolism
    Chemische Substanzen Ribosomal Proteins
    Sprache Englisch
    Erscheinungsdatum 2020-01-02
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2632264-X
    ISSN 2046-6390
    ISSN 2046-6390
    DOI 10.1242/bio.047233
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel ; Online: Recent studies implicate the nucleolus as the major site of nuclear translation.

    McLeod, Tina / Abdullahi, Akilu / Li, Min / Brogna, Saverio

    Biochemical Society transactions

    2014  Band 42, Heft 4, Seite(n) 1224–1228

    Abstract: The nucleolus is the most prominent morphological feature within the nucleus of eukaryotic cells and is best known for its role in ribosome biogenesis. It forms around highly transcribed ribosomal RNA gene repeats which yield precursor rRNAs that are co- ... ...

    Abstract The nucleolus is the most prominent morphological feature within the nucleus of eukaryotic cells and is best known for its role in ribosome biogenesis. It forms around highly transcribed ribosomal RNA gene repeats which yield precursor rRNAs that are co-transcriptionally processed, folded and, while still within the nucleolus, associate with most of the ribosomal proteins. The nucleolus is therefore often thought of as a factory for making ribosomal subunits, which are exported as inactive precursors to the cytoplasm where late maturation makes them capable of mRNA binding and translation initiation. However, recent studies have shown substantial evidence for the presence of functional, translation competent ribosomal subunits within the nucleus, particularly in the nucleolus. These observations raise the intriguing possibility that the nucleolus, as well as being a ribosome factory, is also an important nuclear protein-synthesis plant.
    Mesh-Begriff(e) Animals ; Cell Nucleolus/metabolism ; Cell Nucleus/metabolism ; Humans ; Ribosomal Proteins/metabolism ; Ribosome Subunits/metabolism ; Ribosomes/metabolism
    Chemische Substanzen Ribosomal Proteins
    Sprache Englisch
    Erscheinungsdatum 2014-08
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 184237-7
    ISSN 1470-8752 ; 0300-5127
    ISSN (online) 1470-8752
    ISSN 0300-5127
    DOI 10.1042/BST20140062
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 944: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.

    Al-Jubran, Khalid / Wen, Jikai / Abdullahi, Akilu / Roy Chaudhury, Subhendu / Li, Min / Ramanathan, Preethi / Matina, Annunziata / De, Sandip / Piechocki, Kim / Rugjee, Kushal Nivriti / Brogna, Saverio

    RNA (New York, N.Y.)

    2013  Band 19, Heft 12, Seite(n) 1669–1683

    Abstract: In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, ... ...

    Abstract In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not. Moreover, the complementation signal is found in ribosomal fractions and it was enhanced by translation elongation inhibitors and reduced by initiation inhibitors. Our technique achieved 80S visualization both in cultured cells and in fly tissues in vivo. Notably, while the main 80S signal was in the cytoplasm, clear signals were also seen in the nucleolus and at other nuclear sites. Furthermore, we detected rapid puromycin incorporation in the nucleolus and at transcription sites, providing an independent indication of functional 80S in the nucleolus and 80S association with nascent transcripts.
    Mesh-Begriff(e) Animals ; Bacterial Proteins/biosynthesis ; Cell Line ; Cell Nucleolus/metabolism ; Cell Nucleus/metabolism ; Drosophila Proteins/metabolism ; Drosophila melanogaster/cytology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Luminescent Proteins/biosynthesis ; Microscopy, Fluorescence ; Peptidyl Transferases/metabolism ; Polytene Chromosomes/metabolism ; Protein Binding ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Recombinant Fusion Proteins/biosynthesis ; Ribosomal Proteins/metabolism ; Ribosomes/metabolism ; Transcription, Genetic
    Chemische Substanzen Bacterial Proteins ; Drosophila Proteins ; Luminescent Proteins ; RNA, Messenger ; Recombinant Fusion Proteins ; Ribosomal Proteins ; yellow fluorescent protein, Bacteria ; Peptidyl Transferases (EC 2.3.2.12)
    Sprache Englisch
    Erscheinungsdatum 2013-10-15
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1241540-6
    ISSN 1469-9001 ; 1355-8382
    ISSN (online) 1469-9001
    ISSN 1355-8382
    DOI 10.1261/rna.038356.113
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 4777: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang