Artikel ; Online: Continuous Real-Time Detection of Serotonin Using an Aptamer-Based Electrochemical Biosensor.
2023 Band 13, Heft 11
Abstract: Serotonin (5-HT) is a critical neurotransmitter involved in many neuronal functions, and 5-HT depletion has been linked to several mental diseases. The fast release and clearance of serotonin in the extracellular space, low analyte concentrations, and a ... ...
Abstract | Serotonin (5-HT) is a critical neurotransmitter involved in many neuronal functions, and 5-HT depletion has been linked to several mental diseases. The fast release and clearance of serotonin in the extracellular space, low analyte concentrations, and a multitude of interfering species make the detection of serotonin challenging. This work presents an electrochemical aptamer-based biosensing platform that can monitor 5-HT continuously with high sensitivity and selectivity. Our electrochemical sensor showed a response time of approximately 1 min to a step change in the serotonin concentration in continuous monitoring using a single-frequency EIS (electrochemical impedance spectroscopy) technique. The developed sensing platform was able to detect 5-HT in the range of 25-150 nM in the continuous sample fluid flow with a detection limit (LOD) of 5.6 nM. The electrochemical sensor showed promising selectivity against other species with similar chemical structures and redox potentials, including dopamine (DA), norepinephrine (NE), L-tryptophan (L-TP), 5-hydroxyindoleacetic acid (5-HIAA), and 5-hydroxytryptophan (5-HTP). The proposed sensing platform is able to achieve high selectivity in the nanomolar range continuously in real-time, demonstrating the potential for monitoring serotonin from neurons in organ-on-a-chip or brain-on-a-chip-based platforms. |
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Mesh-Begriff(e) | Serotonin ; Dopamine ; Norepinephrine ; Tryptophan ; Oligonucleotides ; Biosensing Techniques ; Electrochemical Techniques |
Chemische Substanzen | Serotonin (333DO1RDJY) ; Dopamine (VTD58H1Z2X) ; Norepinephrine (X4W3ENH1CV) ; Tryptophan (8DUH1N11BX) ; Oligonucleotides |
Sprache | Englisch |
Erscheinungsdatum | 2023-11-13 |
Erscheinungsland | Switzerland |
Dokumenttyp | Journal Article |
ZDB-ID | 2662125-3 |
ISSN | 2079-6374 ; 2079-6374 |
ISSN (online) | 2079-6374 |
ISSN | 2079-6374 |
DOI | 10.3390/bios13110983 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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