Artikel ; Online: Long-Term Effects of Low-Dose Naltrexone on Immunomodulatory Properties of Human Adipose-Derived Mesenchymal Stem Cells.
Iranian journal of immunology : IJI
2023 Band 20, Heft 2, Seite(n) 219–231
Abstract: Background: Low-dose naltrexone (LDN) is involved in the treatment of inflammatory and immune system diseases and can affect immune cells. Mesenchymal stem cells (MSCs) are known for their immunomodulatory effects and the potential for the treatment of ... ...
| Abstract | Background: Low-dose naltrexone (LDN) is involved in the treatment of inflammatory and immune system diseases and can affect immune cells. Mesenchymal stem cells (MSCs) are known for their immunomodulatory effects and the potential for the treatment of certain types of autoimmune diseases. Objective: To investigate the long-term effects of LDN on human adipose-derived mesenchymal stem cells (ASCs) to see how their immunomodulatory properties are affected and also how LDN-treated ASCs interact with other immune cells present in peripheral blood mononuclear cells (PBMCs). Methods: After 14 days of treatment, the ability of LDN-treated ASCs to modulate PBMC proliferation in a two-way mixed lymphocyte reaction (MLR) model was assessed using XTT. The relative expression of IDO, PD-L1, COX-2, HGF genes, and the level of IL-6 and TGF-β cytokines were measured in IFN-γ stimulated and unstimulated ASCs (treated and not treated cells) using real-time PCR and ELISA respectively. Results: Unstimulated ASCs treated with 10-8 M Naltrexone (10-8 M NTX) showed higher levels of TGF-β, compared with the controls (P<0.05). Stimulated ASCs treated with 10-6 M NTX showed elevated expression of IDO, PD-L1 genes, and IL-6 level (P<0.05). Conclusion: Our results demonstrated that various LDN concentrations have dissimilar effects on ASCs' immunomodulatory properties. A higher LDN concentration induced an alteration in the immunomodulatory features of ASCs. |
|---|---|
| Mesh-Begriff(e) | Humans ; Adipose Tissue/metabolism ; Naltrexone/pharmacology ; Naltrexone/metabolism ; Leukocytes, Mononuclear ; B7-H1 Antigen/genetics ; B7-H1 Antigen/metabolism ; Interleukin-6/metabolism ; Transforming Growth Factor beta ; Mesenchymal Stem Cells ; Cells, Cultured |
| Chemische Substanzen | Naltrexone (5S6W795CQM) ; B7-H1 Antigen ; Interleukin-6 ; Transforming Growth Factor beta |
| Sprache | Englisch |
| Erscheinungsdatum | 2023-05-08 |
| Erscheinungsland | Iran |
| Dokumenttyp | Journal Article |
| ZDB-ID | 2616647-1 |
| ISSN | 1735-367X ; 1735-367X |
| ISSN (online) | 1735-367X |
| ISSN | 1735-367X |
| DOI | 10.22034/iji.2023.95659.2385 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.