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  1. Artikel: Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction

    Beltrán-Pavez, Carolina / Alonso-Palomares, Luis A. / Valiente-Echeverría, Fernando / Gaggero, Aldo / Soto-Rifo, Ricardo / Barriga, Gonzalo P.

    Journal of virological methods. 2021 Jan., v. 287

    2021  

    Abstract: The current COVID‐19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected ... ...

    Abstract The current COVID‐19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected individuals. we sought to evaluate different RT-qPCR kits and protocols to evaluate the best approach to be used omitting an RNA extraction step. We have investigated the sensitivity and performance of different commercially available RT-qPCR kits in detecting SARS-CoV-2 using 80 extracted RNA and NSS from COVID-19 diagnosed patients. We evaluated the ability of each kit to detect viral RNA from both kit-extracted or directly from a pre-boiled NSS observing that direct RNA detection is possible when Ct values are lower than 30 with the three kits tested. Since SARS-CoV-2 testing in most locations occurs once COVID-19 symptoms are evident and, therefore, viral loads are expected to be high, our protocol will be useful in supporting SARS-CoV-2 diagnosis, especially in America where COVID-19 cases have exploded in the recent weeks as well as in low- and middle-income countries, which would not have massive access to kit-based diagnosis. The information provided in this work paves the way for the development of more efficient SARS-CoV-2 detection approaches avoiding an RNA extraction step.
    Schlagwörter COVID-19 infection ; RNA ; Severe acute respiratory syndrome coronavirus 2
    Sprache Englisch
    Erscheinungsverlauf 2021-01
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2020.113969
    Datenquelle NAL Katalog (AGRICOLA)

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  2. Artikel ; Online: Molecular Basis for Arbovirus Transmission by Aedes aegypti Mosquitoes.

    Alonso-Palomares, Luis A / Moreno-García, Miguel / Lanz-Mendoza, Humberto / Salazar, Ma Isabel

    Intervirology

    2019  Band 61, Heft 6, Seite(n) 255–264

    Abstract: Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of ... ...

    Abstract Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of pathogenic viruses. Examples are dengue, yellow fever, chikungunya, Zika, and Mayaro viruses. After ingesting viremic blood, a wide range of mechanisms are activated in the mosquito to counteract viral infection. Nevertheless, these arboviruses possess strategies to overcome barriers in the mosquito and eventually reach the salivary glands to continue the transmission cycle. However, the infection and eventual transmission of arbovirus depends on multiple factors. The current review focuses in detail on the anatomic, physiological, and molecular characteristics of the mosquito A. aegypti that participate in response to a viral infection. In the past decades, the awareness of the importance of this mosquito as a disease vector and its impact on human health was largely recognized. We need to improve our comprehension of molecular mechanisms that determine the outcome of successful virus replication or control of infection for each arbovirus in the vector; this could lead to the design of effective control strategies in the future.
    Mesh-Begriff(e) Aedes/virology ; Animals ; Arbovirus Infections/genetics ; Arbovirus Infections/metabolism ; Arbovirus Infections/transmission ; Arbovirus Infections/virology ; Arboviruses/physiology ; Biomarkers ; Disease Susceptibility ; Gene Expression Regulation ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/immunology ; Humans ; Mosquito Vectors/virology ; RNA Interference ; Signal Transduction
    Chemische Substanzen Biomarkers
    Sprache Englisch
    Erscheinungsdatum 2019-05-13
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 184545-7
    ISSN 1423-0100 ; 0300-5526
    ISSN (online) 1423-0100
    ISSN 0300-5526
    DOI 10.1159/000499128
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction.

    Beltrán-Pavez, Carolina / Alonso-Palomares, Luis A / Valiente-Echeverría, Fernando / Gaggero, Aldo / Soto-Rifo, Ricardo / Barriga, Gonzalo P

    Journal of virological methods

    2020  Band 287, Seite(n) 113969

    Abstract: The current COVID-19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected ... ...

    Abstract The current COVID-19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected individuals. we sought to evaluate different RT-qPCR kits and protocols to evaluate the best approach to be used omitting an RNA extraction step. We have investigated the sensitivity and performance of different commercially available RT-qPCR kits in detecting SARS-CoV-2 using 80 extracted RNA and NSS from COVID-19 diagnosed patients. We evaluated the ability of each kit to detect viral RNA from both kit-extracted or directly from a pre-boiled NSS observing that direct RNA detection is possible when Ct values are lower than 30 with the three kits tested. Since SARS-CoV-2 testing in most locations occurs once COVID-19 symptoms are evident and, therefore, viral loads are expected to be high, our protocol will be useful in supporting SARS-CoV-2 diagnosis, especially in America where COVID-19 cases have exploded in the recent weeks as well as in low- and middle-income countries, which would not have massive access to kit-based diagnosis. The information provided in this work paves the way for the development of more efficient SARS-CoV-2 detection approaches avoiding an RNA extraction step.
    Mesh-Begriff(e) COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Nucleic Acid Testing/methods ; Humans ; Nasopharynx/virology ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Reagent Kits, Diagnostic ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Specimen Handling/methods ; Time Factors ; Viral Load
    Chemische Substanzen RNA, Viral ; Reagent Kits, Diagnostic
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-09-09
    Erscheinungsland Netherlands
    Dokumenttyp Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2020.113969
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Surveillance of seasonal respiratory viruses among Chilean patients during the COVID-19 pandemic.

    Alonso-Palomares, Luis A / Cáceres, C Joaquín / Tapia, Rodrigo / Aguilera-Cortés, Paulina / Valenzuela, Santiago / Valiente-Echeverría, Fernando / Soto-Rifo, Ricardo / Gaggero, Aldo / Barriga, Gonzalo P

    Health science reports

    2021  Band 4, Heft 4, Seite(n) e433

    Sprache Englisch
    Erscheinungsdatum 2021-11-23
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2398-8835
    ISSN (online) 2398-8835
    DOI 10.1002/hsr2.433
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: Molecular Basis for Arbovirus Transmission by ; Mosquitoes

    Alonso-Palomares, Luis A. / Moreno-García, Miguel / Lanz-Mendoza, Humberto / Salazar, Ma Isabel

    Intervirology

    2019  Band 61, Heft 6, Seite(n) 255–264

    Abstract: Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of ... ...

    Körperschaft Laboratorio de Virología e Inmunovirología, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional (ENCB-IPN), Unidad Profesional “Lázaro Cárdenas”, Mexico City, Mexico
    Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública (CISEI-INSP), Cuernavaca, Mexico
    Centro Regional de Control de Vectores, Secretaría de Salud (CERECOVE-SS), Panchimalco, Mexico
    Abstract Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of pathogenic viruses. Examples are dengue, yellow fever, chikungunya, Zika, and Mayaro viruses. After ingesting viremic blood, a wide range of mechanisms are activated in the mosquito to counteract viral infection. Nevertheless, these arboviruses possess strategies to overcome barriers in the mosquito and eventually reach the salivary glands to continue the transmission cycle. However, the infection and eventual transmission of arbovirus depends on multiple factors. The current review focuses in detail on the anatomic, physiological, and molecular characteristics of the mosquito A. aegypti that participate in response to a viral infection. In the past decades, the awareness of the importance of this mosquito as a disease vector and its impact on human health was largely recognized. We need to improve our comprehension of molecular mechanisms that determine the outcome of successful virus replication or control of infection for each arbovirus in the vector; this could lead to the design of effective control strategies in the future.
    Schlagwörter Arbovirus ; Mosquitoes ; Transmission ; Determinants
    Sprache Englisch
    Erscheinungsdatum 2019-05-13
    Verlag S. Karger AG
    Erscheinungsort Basel, Switzerland
    Dokumenttyp Artikel
    Anmerkung Review
    ZDB-ID 184545-7
    ISSN 1423-0100 ; 0300-5526
    ISSN (online) 1423-0100
    ISSN 0300-5526
    DOI 10.1159/000499128
    Datenquelle Karger Verlag

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  6. Artikel: Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction

    Beltrán-Pavez, Carolina / Alonso-Palomares, Luis A / Valiente-Echeverría, Fernando / Gaggero, Aldo / Soto-Rifo, Ricardo / Barriga, Gonzalo P

    J Virol Methods

    Abstract: The current COVID-19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected ... ...

    Abstract The current COVID-19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected individuals. we sought to evaluate different RT-qPCR kits and protocols to evaluate the best approach to be used omitting an RNA extraction step. We have investigated the sensitivity and performance of different commercially available RT-qPCR kits in detecting SARS-CoV-2 using 80 extracted RNA and NSS from COVID-19 diagnosed patients. We evaluated the ability of each kit to detect viral RNA from both kit-extracted or directly from a pre-boiled NSS observing that direct RNA detection is possible when Ct values are lower than 30 with the three kits tested. Since SARS-CoV-2 testing in most locations occurs once COVID-19 symptoms are evident and, therefore, viral loads are expected to be high, our protocol will be useful in supporting SARS-CoV-2 diagnosis, especially in America where COVID-19 cases have exploded in the recent weeks as well as in low- and middle-income countries, which would not have massive access to kit-based diagnosis. The information provided in this work paves the way for the development of more efficient SARS-CoV-2 detection approaches avoiding an RNA extraction step.
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #753173
    Datenquelle COVID19

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  7. Artikel ; Online: Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction

    Beltrán-Pavez, Carolina / Alonso-Palomares, Luis A. / Valiente-Echeverría, Fernando / Gaggero, Aldo / Soto-Rifo, Ricardo / Barriga, Gonzalo P.

    Journal of Virological Methods

    Band 287, Seite(n) 113969

    Schlagwörter Virology ; covid19
    Sprache Englisch
    Verlag Elsevier BV
    Erscheinungsland us
    Dokumenttyp Artikel ; Online
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2020.113969
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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