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  1. Artikel ; Online: Beware imposters: MA-1, a novel MALT lymphoma cell line, is misidentified and corresponds to Pfeiffer, a diffuse large B-cell lymphoma cell line.

    Capes-Davis, Amanda / Alston-Roberts, Christine / Kerrigan, Liz / Reid, Yvonne A / Barrett, Tanya / Burnett, Edward C / Cooper, Jim R / Freshney, R Ian / Healy, Lyn / Kohara, Arihiro / Korch, Christopher / Masters, John R W / Nakamura, Yukio / Nims, Raymond W / Storts, Douglas R / Dirks, Wilhelm G / MacLeod, Roderick A F / Drexler, Hans G

    Genes, chromosomes & cancer

    2013  Band 52, Heft 10, Seite(n) 986–988

    Mesh-Begriff(e) Cell Line, Tumor ; Humans ; Lymphoma, B-Cell, Marginal Zone/pathology ; Lymphoma, Non-Hodgkin/pathology ; Male ; Stomach Neoplasms/pathology
    Sprache Englisch
    Erscheinungsdatum 2013-10
    Erscheinungsland United States
    Dokumenttyp Comment ; Letter
    ZDB-ID 1018988-9
    ISSN 1098-2264 ; 1045-2257
    ISSN (online) 1098-2264
    ISSN 1045-2257
    DOI 10.1002/gcc.22094
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Match criteria for human cell line authentication: where do we draw the line?

    Capes-Davis, Amanda / Reid, Yvonne A / Kline, Margaret C / Storts, Douglas R / Strauss, Ethan / Dirks, Wilhelm G / Drexler, Hans G / MacLeod, Roderick A F / Sykes, Gregory / Kohara, Arihiro / Nakamura, Yukio / Elmore, Eugene / Nims, Raymond W / Alston-Roberts, Christine / Barallon, Rita / Los, Georgyi V / Nardone, Roland M / Price, Paul J / Steuer, Anton /
    Thomson, Jim / Masters, John R W / Kerrigan, Liz

    International journal of cancer

    2013  Band 132, Heft 11, Seite(n) 2510–2519

    Abstract: Continuous human cell lines have been used extensively as models for biomedical research. In working with these cell lines, researchers are often unaware of the risk of cross-contamination and other causes of misidentification. To reduce this risk, there ...

    Abstract Continuous human cell lines have been used extensively as models for biomedical research. In working with these cell lines, researchers are often unaware of the risk of cross-contamination and other causes of misidentification. To reduce this risk, there is a pressing need to authenticate cell lines, comparing the sample handled in the laboratory to a previously tested sample. The American Type Culture Collection Standards Development Organization Workgroup ASN-0002 has developed a Standard for human cell line authentication, recommending short tandem repeat (STR) profiling for authentication of human cell lines. However, there are known limitations to the technique when applied to cultured samples, including possible genetic drift with passage. In our study, a dataset of 2,279 STR profiles from four cell banks was used to assess the effectiveness of the match criteria recommended within the Standard. Of these 2,279 STR profiles, 1,157 were grouped into sets of related cell lines-duplicate holdings, legitimately related samples or misidentified cell lines. Eight core STR loci plus amelogenin were used to unequivocally authenticate 98% of these related sets. Two simple match algorithms each clearly discriminated between related and unrelated samples, with separation between related samples at ≥80% match and unrelated samples at <50% match. A small degree of overlap was noted at 50-79% match, mostly from cell lines known to display variable STR profiles. These match criteria are recommended as a simple and effective way to interpret results from STR profiling of human cell lines.
    Mesh-Begriff(e) Algorithms ; Cell Line ; Gene Expression Profiling/methods ; Genotyping Techniques/standards ; Humans ; Microsatellite Repeats/genetics ; Polymerase Chain Reaction
    Sprache Englisch
    Erscheinungsdatum 2013-06-01
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 218257-9
    ISSN 1097-0215 ; 0020-7136
    ISSN (online) 1097-0215
    ISSN 0020-7136
    DOI 10.1002/ijc.27931
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues

    Barallon, Rita / Bauer, Steven R / Butler, John / Capes-Davis, Amanda / Dirks, Wilhelm G / Elmore, Eugene / Furtado, Manohar / Kline, Margaret C / Kohara, Arihiro / Los, Georgyi V / MacLeod, Roderick A. F / Masters, John R. W / Nardone, Mark / Nardone, Roland M / Nims, Raymond W / Price, Paul J / Reid, Yvonne A / Shewale, Jaiprakash / Sykes, Gregory /
    Steuer, Anton F / Storts, Douglas R / Thomson, Jim / Taraporewala, Zenobia / Alston-Roberts, Christine / Kerrigan, Liz

    In vitro cellular & developmental biology. Animal. 2010 Oct., v. 46, no. 9

    2010  

    Abstract: Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising ... ...

    Abstract Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues.
    Sprache Englisch
    Erscheinungsverlauf 2010-10
    Umfang p. 727-732.
    Verlag Springer-Verlag
    Erscheinungsort New York
    Dokumenttyp Artikel
    ISSN 1071-2690
    DOI 10.1007/s11626-010-9333-z
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel ; Online: Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues.

    Barallon, Rita / Bauer, Steven R / Butler, John / Capes-Davis, Amanda / Dirks, Wilhelm G / Elmore, Eugene / Furtado, Manohar / Kline, Margaret C / Kohara, Arihiro / Los, Georgyi V / MacLeod, Roderick A F / Masters, John R W / Nardone, Mark / Nardone, Roland M / Nims, Raymond W / Price, Paul J / Reid, Yvonne A / Shewale, Jaiprakash / Sykes, Gregory /
    Steuer, Anton F / Storts, Douglas R / Thomson, Jim / Taraporewala, Zenobia / Alston-Roberts, Christine / Kerrigan, Liz

    In vitro cellular & developmental biology. Animal

    2010  Band 46, Heft 9, Seite(n) 727–732

    Abstract: Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising ... ...

    Abstract Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues.
    Mesh-Begriff(e) Cell Biology/standards ; Cell Line ; Gene Expression Profiling/methods ; Humans ; Microsatellite Repeats/genetics ; Specimen Handling/methods ; Stem Cells ; Tissue Banks/standards ; United States
    Sprache Englisch
    Erscheinungsdatum 2010-07-08
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 1077810-x
    ISSN 1543-706X ; 0883-8364 ; 1071-2690
    ISSN (online) 1543-706X
    ISSN 0883-8364 ; 1071-2690
    DOI 10.1007/s11626-010-9333-z
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 851: Hefte anzeigen Standort:
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