Artikel ; Online: Glucogallin Attenuates the LPS-Induced Signaling in Macrophages and Protects Mice against Sepsis.
International journal of molecular sciences
2022 Band 23, Heft 19
Abstract: The anti-oxidant and anti-inflammatory effect of beta-glucogallin (BGG), a plant-derived natural product, was evaluated in both in vitro and in vivo studies. For the in vitro study, the ability of BGG pre-treatment to quench LPS-induced effects compared ... ...
Abstract | The anti-oxidant and anti-inflammatory effect of beta-glucogallin (BGG), a plant-derived natural product, was evaluated in both in vitro and in vivo studies. For the in vitro study, the ability of BGG pre-treatment to quench LPS-induced effects compared to LPS alone in macrophages was investigated. It was found that BGG pre-treatment showed a significant decrease in ROS, NO, superoxide, and pro-inflammatory cytokines (TNF-alpha, IL-4, IL-17, IL-1β, and IL-6) and increased reduced glutathione coupled with the restoration of mitochondrial membrane potential. Gene profiling and further validation by qPCR showed that BGG pre-treatment downregulated the LPS-induced expression of c-Fos, Fas, MMP-9, iNOS, COX-2, MyD88, TRIF, TRAF6, TRAM, c-JUN, and NF-κB. We observed that BGG pre-treatment reduced nuclear translocation of LPS-activated NF-κB and thus reduced the subsequent expressions of NLRP3 and IL-1β, indicating the ability of BGG to inhibit inflammasome formation. Molecular docking studies showed that BGG could bind at the active site of TLR4. Finally, in the LPS-driven sepsis mouse model, we showed that pre-treatment with BGG sustained toxic shock, as evident from their 100% survival. Our study clearly showed the therapeutic potential of BGG in toxic shock syndrome. |
---|---|
Mesh-Begriff(e) | Adaptor Proteins, Vesicular Transport/metabolism ; Animals ; Anti-Inflammatory Agents/adverse effects ; Antioxidants/pharmacology ; Biological Products/pharmacology ; Cyclooxygenase 2/metabolism ; Cytokines/metabolism ; Glutathione/metabolism ; Hydrolyzable Tannins ; Inflammasomes/metabolism ; Interleukin-17/metabolism ; Interleukin-4/metabolism ; Interleukin-6/metabolism ; Lipopolysaccharides/adverse effects ; Macrophages/metabolism ; Matrix Metalloproteinase 9/metabolism ; Mice ; Molecular Docking Simulation ; Myeloid Differentiation Factor 88/metabolism ; NF-kappa B/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Reactive Oxygen Species/metabolism ; Sepsis/metabolism ; Superoxides/metabolism ; TNF Receptor-Associated Factor 6/metabolism ; Toll-Like Receptor 4/metabolism ; Tumor Necrosis Factor-alpha/metabolism |
Chemische Substanzen | Adaptor Proteins, Vesicular Transport ; Anti-Inflammatory Agents ; Antioxidants ; Biological Products ; Cytokines ; Hydrolyzable Tannins ; Inflammasomes ; Interleukin-17 ; Interleukin-6 ; Lipopolysaccharides ; Myeloid Differentiation Factor 88 ; NF-kappa B ; NLR Family, Pyrin Domain-Containing 3 Protein ; Reactive Oxygen Species ; TNF Receptor-Associated Factor 6 ; Toll-Like Receptor 4 ; Tumor Necrosis Factor-alpha ; Superoxides (11062-77-4) ; Interleukin-4 (207137-56-2) ; glucogallin (58511-73-2) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Glutathione (GAN16C9B8O) |
Sprache | Englisch |
Erscheinungsdatum | 2022-09-24 |
Erscheinungsland | Switzerland |
Dokumenttyp | Journal Article |
ZDB-ID | 2019364-6 |
ISSN | 1422-0067 ; 1422-0067 ; 1661-6596 |
ISSN (online) | 1422-0067 |
ISSN | 1422-0067 ; 1661-6596 |
DOI | 10.3390/ijms231911254 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.