Artikel: Ceruloplasmin has two nearly identical sites that bind myeloperoxidase
Biochemical and biophysical research communications. 2014 Oct. 31, v. 453
2014
Abstract: Ceruloplasmin (Cp) is a copper-containing ferroxidase with potent antioxidant activity. Cp is expressed by hepatocytes and activated macrophages and has been known as physiologic inhibitor of myeloperoxidase (MPO). Enzymatic activity of MPO produces anti- ...
Abstract | Ceruloplasmin (Cp) is a copper-containing ferroxidase with potent antioxidant activity. Cp is expressed by hepatocytes and activated macrophages and has been known as physiologic inhibitor of myeloperoxidase (MPO). Enzymatic activity of MPO produces anti-microbial agents and strong prooxidants such as hypochlorous acid and has a potential to damage host tissue at the sites of inflammation and infection. Thus Cp–MPO interaction and inhibition of MPO has previously been suggested as an important control mechanism of excessive MPO activity. Our aim in this study was to identify minimal Cp domain or peptide that interacts with MPO. We first confirmed Cp–MPO interaction by ELISA and surface plasmon resonance (SPR). SPR analysis of the interaction yielded 30nM affinity between Cp and MPO. We then designed and synthesized 87 overlapping peptides spanning the entire amino acid sequence of Cp. Each of the peptides was tested whether it binds to MPO by direct binding ELISA. Two of the 87 peptides, P18 and P76 strongly interacted with MPO. Amino acid sequence analysis of identified peptides revealed high sequence and structural homology between them. Further structural analysis of Cp’s crystal structure by PyMOL software unfolded that both peptides represent surface-exposed sites of Cp and face nearly the same direction. To confirm our finding we raised anti-P18 antisera in rabbit and demonstrated that this antisera disrupts Cp–MPO binding and rescues MPO activity. Collectively, our results confirm Cp–MPO interaction and identify two nearly identical sites on Cp that specifically bind MPO. We propose that inhibition of MPO by Cp requires two nearly identical sites on Cp to bind homodimeric MPO simultaneously and at an angle of at least 120degrees, which, in turn, exerts tension on MPO and results in conformational change. |
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Schlagwörter | amino acid sequences ; anti-infective agents ; antioxidant activity ; antiserum ; computer software ; crystal structure ; enzyme activity ; enzyme-linked immunosorbent assay ; ferroxidase ; hepatocytes ; inflammation ; macrophages ; myeloperoxidase ; peptides ; rabbits ; sequence analysis ; surface plasmon resonance |
Sprache | Englisch |
Erscheinungsverlauf | 2014-1031 |
Umfang | p. 722-727. |
Erscheinungsort | Elsevier Inc. |
Dokumenttyp | Artikel |
ZDB-ID | 205723-2 |
ISSN | 0006-291X ; 0006-291X |
ISSN (online) | 0006-291X |
ISSN | 0006-291X |
DOI | 10.1016/j.bbrc.2014.09.134 |
Datenquelle | NAL Katalog (AGRICOLA) |
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