Artikel ; Online: Resolving the intricate binding of neomycin B to multiple binding motifs of a neomycin-sensing riboswitch aptamer by native top-down mass spectrometry and NMR spectroscopy.
2024 Band 52, Heft 8, Seite(n) 4691–4701
Abstract: Understanding small molecule binding to RNA can be complicated by an intricate interplay between binding stoichiometry, multiple binding motifs, different occupancies of different binding motifs, and changes in the structure of the RNA under study. Here, ...
| Abstract | Understanding small molecule binding to RNA can be complicated by an intricate interplay between binding stoichiometry, multiple binding motifs, different occupancies of different binding motifs, and changes in the structure of the RNA under study. Here, we use native top-down mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy to experimentally resolve these factors and gain a better understanding of the interactions between neomycin B and the 40 nt aptamer domain of a neomycin-sensing riboswitch engineered in yeast. Data from collisionally activated dissociation of the 1:1, 1:2 and 1:3 RNA-neomycin B complexes identified a third binding motif C of the riboswitch in addition to the two motifs A and B found in our previous study, and provided occupancies of the different binding motifs for each complex stoichiometry. Binding of a fourth neomycin B molecule was unspecific according to both MS and NMR data. Intriguingly, all major changes in the aptamer structure can be induced by the binding of the first neomycin B molecule regardless of whether it binds to motif A or B as evidenced by stoichiometry-resolved MS data together with titration data from 1H NMR spectroscopy in the imino proton region. Specific binding of the second and third neomycin B molecules further stabilizes the riboswitch aptamer, thereby allowing for a gradual response to increasing concentrations of neomycin B, which likely leads to a fine-tuning of the cellular regulatory mechanism. |
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| Mesh-Begriff(e) | Aptamers, Nucleotide/chemistry ; Aptamers, Nucleotide/metabolism ; Aptamers, Nucleotide/genetics ; Binding Sites ; Framycetin/chemistry ; Framycetin/metabolism ; Magnetic Resonance Spectroscopy/methods ; Mass Spectrometry/methods ; Nuclear Magnetic Resonance, Biomolecular ; Nucleic Acid Conformation ; Nucleotide Motifs ; Riboswitch |
| Chemische Substanzen | Aptamers, Nucleotide ; Framycetin (4BOC774388) ; Riboswitch |
| Sprache | Englisch |
| Erscheinungsdatum | 2024-04-03 |
| Erscheinungsland | England |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 186809-3 |
| ISSN | 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048 |
| ISSN (online) | 1362-4962 ; 1362-4954 |
| ISSN | 0301-5610 ; 0305-1048 |
| DOI | 10.1093/nar/gkae224 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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