Artikel ; Online: The mRNA-bound proteome of the early fly embryo.
2016 Band 26, Heft 7, Seite(n) 1000–1009
Abstract: Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins ( ... ...
| Abstract | Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins (RBPs) is the dominant force in embryo patterning. We used two mRNA interactome capture methods to identify RBPs bound to polyadenylated transcripts within the first 2 h of Drosophila melanogaster embryogenesis. We identified a high-confidence set of 476 putative RBPs and confirmed RNA-binding activities for most of 24 tested candidates. Most proteins in the interactome are known RBPs or harbor canonical RBP features, but 99 exhibited previously uncharacterized RNA-binding activity. mRNA-bound RBPs and TFs exhibit distinct expression dynamics, in which the newly identified RBPs dominate the first 2 h of embryonic development. Integrating our resource with in situ hybridization data from existing databases showed that mRNAs encoding RBPs are enriched in posterior regions of the early embryo, suggesting their general importance in posterior patterning and germ cell maturation. |
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| Mesh-Begriff(e) | Animals ; Drosophila Proteins/metabolism ; Drosophila melanogaster/embryology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Embryonic Development ; Female ; Gene Expression Regulation, Developmental ; Male ; Protein Binding ; Proteome/metabolism ; RNA, Messenger/metabolism ; RNA-Binding Proteins/metabolism | ||||||||||
| Chemische Substanzen | Drosophila Proteins ; Proteome ; RNA, Messenger ; RNA-Binding Proteins | ||||||||||
| Sprache | Englisch | ||||||||||
| Erscheinungsdatum | 2016 | ||||||||||
| Erscheinungsland | United States | ||||||||||
| Dokumenttyp | Journal Article ; Research Support, N.I.H., Extramural | ||||||||||
| ZDB-ID | 1284872-4 | ||||||||||
| ISSN | 1549-5469 ; 1088-9051 ; 1054-9803 | ||||||||||
| ISSN (online) | 1549-5469 | ||||||||||
| ISSN | 1088-9051 ; 1054-9803 | ||||||||||
| DOI | 10.1101/gr.200386.115 | ||||||||||
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| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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