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  1. Artikel: Some Considerations on the Early Diagnosis and Treatment of Mammary Cancer.

    Bonney, Charles W

    Buffalo medical journal

    2023  Band 68, Heft 2, Seite(n) 71–78

    Sprache Englisch
    Erscheinungsdatum 2023-03-08
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 426473-3
    ISSN 1040-3817
    ISSN 1040-3817
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    AMP -385/400-: Hefte anzeigen

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  2. Artikel ; Online: Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.

    Tristan, Carlos A / Ormanoglu, Pinar / Slamecka, Jaroslav / Malley, Claire / Chu, Pei-Hsuan / Jovanovic, Vukasin M / Gedik, Yeliz / Jethmalani, Yogita / Bonney, Charles / Barnaeva, Elena / Braisted, John / Mallanna, Sunil K / Dorjsuren, Dorjbal / Iannotti, Michael J / Voss, Ty C / Michael, Sam / Simeonov, Anton / Singeç, Ilyas

    Stem cell reports

    2021  Band 16, Heft 12, Seite(n) 3076–3092

    Abstract: Efficient translation of human induced pluripotent stem cells (hiPSCs) requires scalable cell manufacturing strategies for optimal self-renewal and functional differentiation. Traditional manual cell culture is variable and labor intensive, posing ... ...

    Abstract Efficient translation of human induced pluripotent stem cells (hiPSCs) requires scalable cell manufacturing strategies for optimal self-renewal and functional differentiation. Traditional manual cell culture is variable and labor intensive, posing challenges for high-throughput applications. Here, we established a robotic platform and automated all essential steps of hiPSC culture and differentiation under chemically defined conditions. This approach allowed rapid and standardized manufacturing of billions of hiPSCs that can be produced in parallel from up to 90 different patient- and disease-specific cell lines. Moreover, we established automated multi-lineage differentiation and generated functional neurons, cardiomyocytes, and hepatocytes. To validate our approach, we compared robotic and manual cell culture operations and performed comprehensive molecular and cellular characterizations (e.g., single-cell transcriptomics, mass cytometry, metabolism, electrophysiology) to benchmark industrial-scale cell culture operations toward building an integrated platform for efficient cell manufacturing for disease modeling, drug screening, and cell therapy.
    Mesh-Begriff(e) Automation ; Cell Culture Techniques/methods ; Cell Differentiation ; Cell Lineage ; Cells, Cultured ; Embryoid Bodies/cytology ; Hepatocytes/cytology ; Hepatocytes/virology ; Human Embryonic Stem Cells/cytology ; Humans ; Induced Pluripotent Stem Cells/cytology ; Myocytes, Cardiac/cytology ; Myocytes, Cardiac/virology ; Neurons/cytology ; RNA-Seq ; Reference Standards ; Robotics ; Single-Cell Analysis ; Zika Virus Infection/pathology
    Sprache Englisch
    Erscheinungsdatum 2021-12-02
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2021.11.004
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells.

    Tristan, Carlos A / Ormanoglu, Pinar / Slamecka, Jaroslav / Malley, Claire / Chu, Pei-Hsuan / Jovanovic, Vukasin M / Gedik, Yeliz / Bonney, Charles / Barnaeva, Elena / Braisted, John / Mallanna, Sunil K / Dorjsuren, Dorjbal / Iannotti, Michael J / Voss, Ty C / Michael, Sam / Simeonov, Anton / Singeç, Ilyas

    bioRxiv : the preprint server for biology

    2020  

    Abstract: Efficient translation of human induced pluripotent stem cells (hiPSCs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation. Currently, manual culture of hiPSCs is highly variable ... ...

    Abstract Efficient translation of human induced pluripotent stem cells (hiPSCs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation. Currently, manual culture of hiPSCs is highly variable and labor-intensive posing significant challenges for high-throughput applications. Here, we established a robotic platform and automated all essential steps of hiPSC culture and differentiation under chemically defined conditions. This streamlined approach allowed rapid and standardized manufacturing of billions of hiPSCs that can be produced in parallel from up to 90 different patient-and disease-specific cell lines. Moreover, we established automated multi-lineage differentiation to generate primary embryonic germ layers and more mature phenotypes such as neurons, cardiomyocytes, and hepatocytes. To validate our approach, we carefully compared robotic and manual cell culture and performed molecular and functional cell characterizations (e.g. bulk culture and single-cell transcriptomics, mass cytometry, metabolism, electrophysiology, Zika virus experiments) in order to benchmark industrial-scale cell culture operations towards building an integrated platform for efficient cell manufacturing for disease modeling, drug screening, and cell therapy. Combining stem cell-based models and non-stop robotic cell culture may become a powerful strategy to increase scientific rigor and productivity, which are particularly important during public health emergencies (e.g. opioid crisis, COVID-19 pandemic).
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-08-03
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2020.08.03.235242
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells

    Tristan, Carlos A. / Ormanoglu, Pinar / Slamecka, Jaroslav / Malley, Claire / Chu, Pei-Hsuan / Jovanovic, Vukasin M. / Gedik, Yeliz / Bonney, Charles / Barnaeva, Elena / Braisted, John / Mallanna, Sunil K. / Dorjsuren, Dorjbal / Iannotti, Michael J. / Voss, Ty C. / Michael, Sam / Simeonov, Anton / Singeç, Ilyas

    bioRxiv : the preprint server for biology

    Abstract: Efficient translation of human induced pluripotent stem cells (hiPSCs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation Currently, manual culture of hiPSCs is highly variable ... ...

    Abstract Efficient translation of human induced pluripotent stem cells (hiPSCs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation Currently, manual culture of hiPSCs is highly variable and labor-intensive posing significant challenges for high-throughput applications Here, we established a robotic platform and automated all essential steps of hiPSC culture and differentiation under chemically defined conditions This streamlined approach allowed rapid and standardized manufacturing of billions of hiPSCs that can be produced in parallel from up to 90 different patient-and disease-specific cell lines Moreover, we established automated multi-lineage differentiation to generate primary embryonic germ layers and more mature phenotypes such as neurons, cardiomyocytes, and hepatocytes To validate our approach, we carefully compared robotic and manual cell culture and performed molecular and functional cell characterizations (e g bulk culture and single-cell transcriptomics, mass cytometry, metabolism, electrophysiology, Zika virus experiments) in order to benchmark industrial-scale cell culture operations towards building an integrated platform for efficient cell manufacturing for disease modeling, drug screening, and cell therapy Combining stem cell-based models and non-stop robotic cell culture may become a powerful strategy to increase scientific rigor and productivity, which are particularly important during public health emergencies (e g opioid crisis, COVID-19 pandemic)
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #721068
    Datenquelle COVID19

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  5. Buch: A text-book of genito-urinary diseases,

    Casper, Leopold / Bonney, Charles W

    including functional sexual disorders in man,

    1909  

    Verfasserangabe by Doctor Leopold Casper ... tr. and ed. with additions by Charles W. Bonney
    Sprache Englisch
    Umfang xvi, 645 p., XXIV pl., ill.
    Ausgabenhinweis 2d ed., rev. and enl., with 230 illustrations and 24 full-page plates, of which 8 are in colors.
    Verlag P. Blakiston's son & co
    Erscheinungsort Philadelphia
    Dokumenttyp Buch
    Anmerkung Translation of Lehrbuch der Urologie.
    Datenquelle Katalog der US National Library of Medicine (NLM)

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  6. Buch: A text-book of genito-urinary diseases,

    Casper, Leopold / Bonney, Charles W

    including functional sexual disorders in man,

    1906  

    Verfasserangabe by Doctor Leopold Casper ... tr. and ed. with additions by Charles W. Bonney ... With 213 illustrations and 23 full page plates, of which 7 are in colors
    Sprache Englisch
    Umfang xii, 618 p., XXIII pl., ill.
    Verlag P. Blakiston's son & co
    Erscheinungsort Philadelphia
    Dokumenttyp Buch
    Anmerkung Translation of Lehrbuch der Urologie.
    Datenquelle Katalog der US National Library of Medicine (NLM)

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  7. Buch ; Online: A summary of the law of marine, fire and life insurance

    Bonney, Charles Carroll

    with practical forms, modern cases, and computing rules

    1865  

    Verfasserangabe by Charles C. Bonney
    Schlagwörter Insurance law ; Insurance law/Forms
    Sprache Englisch
    Umfang Online-Ressource, forms, 23 cm
    Verlag E.B. Myers & Chandler
    Erscheinungsort Chicago
    Dokumenttyp Buch ; Online
    Anmerkung Bibliography: p. 87-101 ; Includes index ; OCLC, 17219609 ; Reproduction of original from Harvard Law School Library
    Datenquelle Ehemaliges Sondersammelgebiet Küsten- und Hochseefischerei

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