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  1. Buch: Amino acid fermentation

    Yokota, Atsushi / Ikeda, Masato / Bott, Michael

    (Advances in biochemical engineering, biotechnology ; 159)

    2017  

    Verfasserangabe Atsushi Yokota, Masato Ikeda, editors ; with contributions by M. Bott, L. Eggeling [und weiteren]
    Serientitel Advances in biochemical engineering, biotechnology ; 159
    Überordnung
    Schlagwörter Amino acid ; Bacteriology ; Biotechnology ; Fermentation ; Industrial production ; Metabolic engineering
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Umfang viii, 310 Seiten, Illustrationen, Diagramme, 23.5 cm x 15.5 cm, 0 g
    Verlag Springer
    Erscheinungsort Tokyo
    Erscheinungsland Japan
    Dokumenttyp Buch
    HBZ-ID HT019350081
    ISBN 978-4-431-56518-5 ; 4-431-56518-3
    Datenquelle Katalog ZB MED Ernährung, Umwelt, Agrar

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  2. Artikel ; Online: Microbial synthesis of health-promoting inositols.

    Yoshida, Ken-Ichi / Bott, Michael

    Current opinion in biotechnology

    2024  Band 87, Seite(n) 103114

    Abstract: D-chiro-inositol and scyllo-inositol are known for their health-promoting properties and promising as ingredients for functional foods. Strains of Bacillus subtilis and Corynebacterium glutamicum were created by metabolic engineering capable of ... ...

    Abstract D-chiro-inositol and scyllo-inositol are known for their health-promoting properties and promising as ingredients for functional foods. Strains of Bacillus subtilis and Corynebacterium glutamicum were created by metabolic engineering capable of inexpensive production of these two rare inositols from myo-inositol, which is the most common inositol in nature. In addition, further modifications have enabled the synthesis of the two rare inositols from the much-cheaper carbon sources, glucose or sucrose.
    Sprache Englisch
    Erscheinungsdatum 2024-03-22
    Erscheinungsland England
    Dokumenttyp Journal Article ; Review
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2024.103114
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Buch ; Online ; Dissertation / Habilitation: Studies on the cAMP-responsive regulatory network of Corynebacterium glutamicum

    Wolf, Natalie [Verfasser] / Bott, Michael [Gutachter]

    2024  

    Verfasserangabe Natalie Wolf ; Gutachter: Michael Bott
    Schlagwörter Biowissenschaften, Biologie ; Life Science, Biology
    Thema/Rubrik (Code) sg570
    Sprache Englisch
    Verlag Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
    Erscheinungsort Düsseldorf
    Dokumenttyp Buch ; Online ; Dissertation / Habilitation
    Datenquelle Digitale Dissertationen im Internet

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  4. Buch: Handbook of corynebacterium glutamicum

    Eggeling, Lothar / Bott, Michael

    2005  

    Verfasserangabe edited by Lothar Eggeling, Michael Bott
    Sprache Englisch
    Umfang 616 S. : Ill., graph. Darst.
    Verlag Taylor & Francis
    Erscheinungsort Boca Raton u.a.
    Erscheinungsland Vereinigte Staaten
    Dokumenttyp Buch
    HBZ-ID HT014124969
    ISBN 0-8493-1821-1 ; 978-0-8493-1821-4
    Datenquelle Katalog ZB MED Ernährung, Umwelt, Agrar

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  5. Buch ; Online ; Dissertation / Habilitation: Analysis of the signal transduction cascade tuning the 2-oxoglutarate dehydrogenase activity in Corynebacterium glutamicum

    Sundermeyer, Lea [Verfasser] / Bott, Michael [Gutachter]

    2023  

    Verfasserangabe Lea Sundermeyer ; Gutachter: Michael Bott
    Schlagwörter Biowissenschaften, Biologie ; Life Science, Biology
    Thema/Rubrik (Code) sg570
    Sprache Englisch
    Verlag Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
    Erscheinungsort Düsseldorf
    Dokumenttyp Buch ; Online ; Dissertation / Habilitation
    Datenquelle Digitale Dissertationen im Internet

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  6. Artikel ; Online: Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes

    Ramp, Paul / Mack, Christina / Wirtz, Astrid / Bott, Michael

    International Metabolic Engineering Society Metabolic Engineering. 2023 July, v. 78 p.1-10

    2023  

    Abstract: d-chiro-Inositol (DCI) is a promising drug candidate for treating insulin resistance and associated diseases such as type 2 diabetes or polycystic ovary syndrome. In this study, we developed two production processes for DCI using Corynebacterium ... ...

    Abstract d-chiro-Inositol (DCI) is a promising drug candidate for treating insulin resistance and associated diseases such as type 2 diabetes or polycystic ovary syndrome. In this study, we developed two production processes for DCI using Corynebacterium glutamicum as host. In the first process, myo-inositol (MI) is oxidized to 2-keto-myo-inositol (2KMI) by the inositol dehydrogenase (IDH) IolG and then isomerized to 1-keto-d-chiro-inositol (1KDCI) by the isomerases Cg0212 or Cg2312, both of which were identified in this work. 1KDCI is then reduced to DCI by IolG. Overproduction of IolG and Cg0212 in a chassis strain unable to degrade inositols allowed the production of 1.1 g/L DCI from 10 g/L MI. As both reactions involved are reversible, only a partial conversion of MI to DCI can be achieved. To enable higher conversion ratios, a novel route towards DCI was established by utilizing the promiscuous activity of two plant-derived enzymes, the NAD⁺-dependent d-ononitol dehydrogenase MtOEPa and the NADPH-dependent d-pinitol dehydrogenase MtOEPb from Medicago truncatula (barrelclover). Heterologous production of these enzymes in the chassis strain led to the production of 1.6 g/L DCI from 10 g/L MI. For replacing the substrate MI by glucose, the two plant genes were co-expressed with the endogenous myo-inositol-1-phosphate synthase gene ino1 either as a synthetic operon or using a novel, bicistronic T7-based expression vector. With the single operon construct, 0.75 g/L DCI was formed from 20 g/L glucose, whereas with the bicistronic construct 1.2 g/L DCI was obtained, disclosing C. glutamicum as an attractive host for of d-chiro-inositol production.
    Schlagwörter Corynebacterium glutamicum ; Medicago truncatula ; drugs ; genetic vectors ; glucose ; inositol-3-phosphate synthase ; insulin resistance ; isomerization ; myo-inositol ; noninsulin-dependent diabetes mellitus ; operon ; oxidoreductases ; polycystic ovary syndrome ; DCI ; MI ; SI ; 2KMI ; 1KDCI ; IDH ; IPTG ; CV
    Sprache Englisch
    Erscheinungsverlauf 2023-07
    Umfang p. 1-10.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel ; Online
    ZDB-ID 1470383-x
    ISSN 1096-7184 ; 1096-7176
    ISSN (online) 1096-7184
    ISSN 1096-7176
    DOI 10.1016/j.ymben.2023.04.013
    Datenquelle NAL Katalog (AGRICOLA)

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  7. Artikel ; Online: Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes.

    Ramp, Paul / Mack, Christina / Wirtz, Astrid / Bott, Michael

    Metabolic engineering

    2023  Band 78, Seite(n) 1–10

    Abstract: d-chiro-Inositol (DCI) is a promising drug candidate for treating insulin resistance and associated diseases such as type 2 diabetes or polycystic ovary syndrome. In this study, we developed two production processes for DCI using Corynebacterium ... ...

    Abstract d-chiro-Inositol (DCI) is a promising drug candidate for treating insulin resistance and associated diseases such as type 2 diabetes or polycystic ovary syndrome. In this study, we developed two production processes for DCI using Corynebacterium glutamicum as host. In the first process, myo-inositol (MI) is oxidized to 2-keto-myo-inositol (2KMI) by the inositol dehydrogenase (IDH) IolG and then isomerized to 1-keto-d-chiro-inositol (1KDCI) by the isomerases Cg0212 or Cg2312, both of which were identified in this work. 1KDCI is then reduced to DCI by IolG. Overproduction of IolG and Cg0212 in a chassis strain unable to degrade inositols allowed the production of 1.1 g/L DCI from 10 g/L MI. As both reactions involved are reversible, only a partial conversion of MI to DCI can be achieved. To enable higher conversion ratios, a novel route towards DCI was established by utilizing the promiscuous activity of two plant-derived enzymes, the NAD
    Mesh-Begriff(e) Humans ; Female ; Corynebacterium glutamicum/genetics ; Corynebacterium glutamicum/metabolism ; Diabetes Mellitus, Type 2/drug therapy ; Inositol/genetics ; Inositol/metabolism ; Polycystic Ovary Syndrome/drug therapy ; Polycystic Ovary Syndrome/metabolism ; Glucose ; Oxidoreductases
    Chemische Substanzen Inositol (4L6452S749) ; Glucose (IY9XDZ35W2) ; Oxidoreductases (EC 1.-)
    Sprache Englisch
    Erscheinungsdatum 2023-05-03
    Erscheinungsland Belgium
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1470383-x
    ISSN 1096-7184 ; 1096-7176
    ISSN (online) 1096-7184
    ISSN 1096-7176
    DOI 10.1016/j.ymben.2023.04.013
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Buch ; Online ; Dissertation / Habilitation: Communities of Niche-optimized Strains (CoNoS) – a novel concept

    Zuchowski, Rico [Verfasser] / Bott, Michael [Gutachter] / Zurbriggen, Matias [Gutachter]

    2024  

    Verfasserangabe Rico Zuchowski ; Gutachter: Michael Bott, Matias Zurbriggen
    Schlagwörter Biowissenschaften, Biologie ; Life Science, Biology
    Thema/Rubrik (Code) sg570
    Sprache Englisch
    Verlag Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
    Erscheinungsort Düsseldorf
    Dokumenttyp Buch ; Online ; Dissertation / Habilitation
    Datenquelle Digitale Dissertationen im Internet

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  9. Artikel ; Online: Vibriosis:: What You and Your Patients Need To Know.

    Bell, Andrew / Bott, Michael

    Delaware journal of public health

    2021  Band 7, Heft 1, Seite(n) 14–21

    Sprache Englisch
    Erscheinungsdatum 2021-01-21
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2639-6378
    ISSN (online) 2639-6378
    DOI 10.32481/djph.2021.001.005
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Buch ; Online ; Dissertation / Habilitation: Strain development of Gluconobacter oxydans and Pseudomonas putida for production of the sweetener 5-ketofructose

    Wohlers, Karen [Verfasser] / Bott, Michael [Gutachter]

    2022  

    Verfasserangabe Karen Wohlers ; Gutachter: Michael Bott
    Schlagwörter Technische Chemie ; Technical Chemistry
    Thema/Rubrik (Code) sg660
    Sprache Englisch
    Verlag Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
    Erscheinungsort Düsseldorf
    Dokumenttyp Buch ; Online ; Dissertation / Habilitation
    Datenquelle Digitale Dissertationen im Internet

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