Artikel ; Online: Characterization of genetic and molecular tools for studying the endogenous expression of Lactate dehydrogenase in Drosophila melanogaster.
2024 Band 19, Heft 1, Seite(n) e0287865
Abstract: Drosophila melanogaster larval development relies on a specialized metabolic state that utilizes carbohydrates and other dietary nutrients to promote rapid growth. One unique feature of the larval metabolic program is that Lactate Dehydrogenase (Ldh) ... ...
Abstract | Drosophila melanogaster larval development relies on a specialized metabolic state that utilizes carbohydrates and other dietary nutrients to promote rapid growth. One unique feature of the larval metabolic program is that Lactate Dehydrogenase (Ldh) activity is highly elevated during this growth phase when compared to other stages of the fly life cycle, indicating that Ldh serves a key role in promoting juvenile development. Previous studies of larval Ldh activity have largely focused on the function of this enzyme at the whole animal level, however, Ldh expression varies significantly among larval tissues, raising the question of how this enzyme promotes tissue-specific growth programs. Here we characterize two transgene reporters and an antibody that can be used to study Ldh expression in vivo. We find that all three tools produce similar Ldh expression patterns. Moreover, these reagents demonstrate that the larval Ldh expression pattern is complex, suggesting the purpose of this enzyme varies across cell types. Overall, our studies validate a series of genetic and molecular reagents that can be used to study glycolytic metabolism in the fly. |
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Mesh-Begriff(e) | Animals ; Drosophila melanogaster ; L-Lactate Dehydrogenase/genetics ; L-Lactate Dehydrogenase/metabolism ; Glycolysis/genetics |
Chemische Substanzen | L-Lactate Dehydrogenase (EC 1.1.1.27) |
Sprache | Englisch |
Erscheinungsdatum | 2024-01-03 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article |
ZDB-ID | 2267670-3 |
ISSN | 1932-6203 ; 1932-6203 |
ISSN (online) | 1932-6203 |
ISSN | 1932-6203 |
DOI | 10.1371/journal.pone.0287865 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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