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Artikel ; Online: Prevalence of salivary anti-SARS-CoV-2 IgG antibodies in vaccinated children.

Badano, María Noel / Duarte, Alejandra / Salamone, Gabriela / Sabbione, Florencia / Pereson, Matias / Chuit, Roberto / Baré, Patricia

Immunology

2023 Band 169, Heft 3, Seite(n) 384–387

Mesh-Begriff(e)	Humans ; Child ; Prevalence ; COVID-19/epidemiology ; Antibodies, Viral ; Vaccination
Chemische Substanzen	Antibodies, Viral
Sprache	Englisch
Erscheinungsdatum	2023-05-15
Erscheinungsland	England
Dokumenttyp	Letter
ZDB-ID	80124-0
ISSN	1365-2567 ; 0019-2805 ; 0953-4954
ISSN (online)	1365-2567
ISSN	0019-2805 ; 0953-4954
DOI	10.1111/imm.13656
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: TNF-α Levels in Respiratory Samples Are Associated with SARS-CoV-2 Infection.

Pereson, Matias J / Badano, Maria Noel / Aloisi, Natalia / Chuit, Roberto / E de Bracco, M M / Bare, Patricia

Microbiology spectrum

2022 Band 10, Heft 1, Seite(n) e0141121

Mesh-Begriff(e)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/immunology ; Child ; Female ; Genome, Viral ; Humans ; Interleukin-6/analysis ; Male ; Middle Aged ; Respiratory System/chemistry ; Respiratory System/virology ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; Tumor Necrosis Factor-alpha/analysis ; Young Adult
Chemische Substanzen	Interleukin-6 ; Tumor Necrosis Factor-alpha
Sprache	Englisch
Erscheinungsdatum	2022-02-09
Erscheinungsland	United States
Dokumenttyp	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.01411-21
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: SARS-CoV-2 Breakthrough Infections after Third Doses Boost IgG Specific Salivary and Blood Antibodies.

Badano, María Noel / Pereson, Matias J / Sabbione, Florencia / Keitelman, Irene / Aloisi, Natalia / Chuit, Roberto / de Bracco, María M E / Fink, Susana / Baré, Patricia

Vaccines

2023 Band 11, Heft 3

Abstract: SARS-CoV-2 breakthrough infections, associated with waning immunity, increase systemic antibody levels. In this study, we analyzed the impact of the infection timing on the magnitude of the systemic humoral response and whether breakthrough infections ... ...

Abstract	SARS-CoV-2 breakthrough infections, associated with waning immunity, increase systemic antibody levels. In this study, we analyzed the impact of the infection timing on the magnitude of the systemic humoral response and whether breakthrough infections also boost antibody levels in the salivary compartment. We observed that the combination of infection plus vaccination, regardless of infection timing, produced a sharp increase in systemic antibodies, which were higher in subjects infected after third doses. Moreover, despite high systemic antibody levels, breakthrough infections after dose three occurred and boosted antibody levels in the salivary compartment. These results suggest that current vaccination strategies against COVID-19 should be improved. Results also showed that determination of salivary antibodies against SARS-CoV-2 could be a valuable tool in disease prevalence studies, for the follow-up of vaccinated individuals, and to assist vaccination strategies against COVID-19, especially in settings where blood sampling cannot be fulfilled.
Sprache	Englisch
Erscheinungsdatum	2023-02-24
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines11030534
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Agenda Nacional de Investigación en Salud Publica

Traverso Vior, Natacha / Chuit , Roberto / Mejía, Raúl

Revista Argentina de Salud Pública, Vol 10, Iss 41, Pp 58-

2019 Band 61

Abstract: El 2 de diciembre de 2019 se presentó la Agenda Nacional de Investigación en Salud Pública en el Ministerio de Salud de Argentina. El establecimiento de prioridades en una Agenda Nacional de Investigación en Salud Pública es un proceso sistemático, ... ...

Abstract	El 2 de diciembre de 2019 se presentó la Agenda Nacional de Investigación en Salud Pública en el Ministerio de Salud de Argentina. El establecimiento de prioridades en una Agenda Nacional de Investigación en Salud Pública es un proceso sistemático, participativo y reflexivo, que permite guiar la investigación hacia problemas relevantes, prevalentes y emergentes de la población, orientar los recursos hacia los temas priorizados, fortalecer y/o crear grupos, centros y redes de investigación para la salud pública, mejorar las capacidades para el desarrollo de investigación de calidad e incrementar la evidencia local para la toma de decisiones a fin de mejorar la salud de la población y los sistemas sanitarios en el marco de los Objetivos de Desarrollo Sustentable 2030. Como metodología para la elaboración de la Agenda se eligió el Método Delphi modificado por la Organización Panamericana de la Salud (OPS). En la elaboración de esta herramienta participaron la Dirección de Investigación para la Salud (DIS) del Ministerio de Salud de la Nación, a través de la Red Ministerial de Investigación en Salud (REMINSA), y la OPS. A lo largo de todo el proceso se consideró imprescindible lograr una amplia participación de actores de los niveles provinciales y nacionales, y de los sectores público y privado. La presencia de una Agenda Nacional de Investigación en Salud Pública permitirá generar investigación científica nacional y regional, e implementar políticas y recomendaciones que impacten en la salud de las poblaciones
Schlagwörter	Medicine (General) ; R5-920 ; Public aspects of medicine ; RA1-1270
Sprache	Spanisch
Erscheinungsdatum	2019-12-01T00:00:00Z
Verlag	Ministerio de Salud
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Characteristics of the treatment received by children with cleft lip and palate in Argentina.

Cipolla, María C / Piola, Agustina / Barbero, Pablo / Groisman, Boris / Bidondo, María P / Chuit, Roberto / Liascovich, Rosa

Andes pediatrica : revista Chilena de pediatria

2021 Band 92, Heft 1, Seite(n) 67–78

Abstract: Introduction: Cleft lip and palate (CLP) are congenital abnormalities that affect anatomically and functionally the face and mouth, involving lip (CL), palate (CP), or both (CL/CP).: Objective: to characterize the treatment of children with CLP in ... ...

Titelübersetzung	Características del tratamiento recibido por los niños con fisura labio alvéolo palatina en Argentina.
Abstract	Introduction: Cleft lip and palate (CLP) are congenital abnormalities that affect anatomically and functionally the face and mouth, involving lip (CL), palate (CP), or both (CL/CP). Objective: to characterize the treatment of children with CLP in public institutions in Argentina. Patients and method: Cross sectional study in a random sample of 100 children from the Flap Network. We included children with isolated CL, CP, and CL/CP, of both sexes, with birth weight of 2500 grams or more and ges tational age over 36 weeks. The following data were recorded date of birth, hospital of birth, birth weight, gestational age, sex, specific diagnosis of the cleft, and initial surgery data. A telephone survey was conducted with the children's parents. To characterize the treatment, three indicators were cons tructed: interdisciplinary, opportunity, and integrality. These indicators were composed of different variables, and according to the sum of the score attributed to each one, the treatment was categorized as high, medium or low based on the treatment guidelines used by the Sumar Program. Results: 30% of the patients started early treatment, 58% underwent timely surgery, and 29% of the children were in follow-up with basic specialties. The mother's high educational level was associated with higher probability of having interdisciplinary (OR2.9; 95%CI 1.3-6.8), comprehensive (OR3.7; 95%CI 1.6 8.7), and timely treatment (OR2.9; 95%CI 1.3-6.7). Conclusions: There are barriers to accessing care, such as long distances or shift management. Less social vulnerability of families was associated with greater likelihood of receiving treatment close to standards.
Mesh-Begriff(e)	Argentina ; Birth Weight ; Child, Preschool ; Cleft Lip/surgery ; Cleft Palate/surgery ; Cross-Sectional Studies ; Educational Status ; Female ; Gestational Age ; Health Services Accessibility ; Humans ; Infant ; Male ; Mothers/education
Sprache	Spanisch
Erscheinungsdatum	2021-02-24
Erscheinungsland	Chile
Dokumenttyp	Journal Article ; Multicenter Study ; Observational Study
ISSN	2452-6053
ISSN (online)	2452-6053
DOI	10.32641/andespediatr.v92i1.2871
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: TNF-α levels in respiratory samples are associated with SARS-CoV-2 infection.

Pereson, Matias Javier / Badano, Maria Noel / Aloisi, Natalia / Chuit, Roberto / Braco, Maria Marta / Bare, Patricia

bioRxiv

Abstract: Purpose: Increased serum levels of IL-6 and TNF-alpha have been proposed as biomarkers for COVID-19 progression. However, the role and the implication of these cytokines in SARS-CoV-2 infection remain controversial. The aim of this study was to measure ... ...

Abstract	Purpose: Increased serum levels of IL-6 and TNF-alpha have been proposed as biomarkers for COVID-19 progression. However, the role and the implication of these cytokines in SARS-CoV-2 infection remain controversial. The aim of this study was to measure levels of IL-6 and TNF-alpha in swab samples from individuals with symptoms compatible with COVID-19 and analyze their association with SARS-CoV-2 presence. Methods: SARS-CoV-2 detection was performed using the CDC (USA) real-time RT-PCR primers, probes and protocols. Cytokine concentrations were measured using commercial reagents based on enzyme linked immunosorbent assay (ELISA). Results: TNF-alpha median levels were greater in COVID19 (+) symptomatic group (5.88 (1.36 - 172.1) pg/ml) compared to COVID19 (-) symptomatic individuals (2.87 (1.45 - 69.9) pg/ml) (p=0.0003). No significant differences were shown in IL-6 median values between COVID-19 (+) and (-) symptomatic patients (5.40 (1.7 - 467) pg/ml and 6.07 (1.57 - 466.6) pg/ml respectively). In addition, increased TNF-alpha; levels (greater than 10 pg/ml), but not IL-6, were associated with SARS-CoV-2 presence (OR= 5.7; p=0.006; 95% CI= 1,551 to 19,11). Conclusions: IL-6 concentration showed high levels in swabs from some symptomatic patients, suggesting the presence of immune response at viral entry site. However, IL-6 levels were independent from SARS-CoV-2 presence and viral load, individual9s age and gender. On the contrary, TNF-alpha evaluation confirmed the presence of inflammatory response but mostly related to COVID-19. More studies are required in order to characterize the cytokine profile expressed at the site of infection of SARS-CoV-2 and its implications in disease outcomes.
Schlagwörter	covid19
Sprache	Englisch
Erscheinungsdatum	2021-07-13
Verlag	Cold Spring Harbor Laboratory
Dokumenttyp	Artikel ; Online
DOI	10.1101/2021.07.12.452071
Datenquelle	COVID19

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Artikel ; Online: Prevalence of salivary anti-SARS-CoV-2 IgG antibodies in vaccinated children

Badano, Maria Noel / Duarte, Alejandra / Salamone, Gabriela / Sabbione, Florencia / Pereson, Matias Javier / Chuit, Roberto / Bare, Patricia

medRxiv

Abstract: Vaccination against COVID-19 has mitigated the impact of SARS-CoV-2 infection, decreasing the probability of progression to severe disease and death in vaccinated people. Parallel to the development and administration of COVID-19 vaccines, the immune ... ...

Abstract	Vaccination against COVID-19 has mitigated the impact of SARS-CoV-2 infection, decreasing the probability of progression to severe disease and death in vaccinated people. Parallel to the development and administration of COVID-19 vaccines, the immune response induced by the different vaccine platforms has been investigated, mainly, in the adult population. However, since the approval of the vaccines for use in pediatric individuals was a posteriori, vaccination began later in this population. This, added to the difficulty in obtaining blood samples from pediatric individuals, has led to less knowledge about the humoral immune response following vaccination in children. In this work, we analyzed the humoral response induced by vaccination in children through a non-invasive approach such as the measurement of specific salivary antibodies. Our results showed a high prevalence of specific salivary antibodies (81%), with the highest levels of antibodies being observed in those children who had three doses, a greater number of exposures and a shorter interval time between the last exposure to SARS-CoV-2 antigens and saliva collection. These results agree with those reported for the systemic humoral immune response in vaccinated adults, suggesting the administration of booster doses in children to maintain high antibody levels. Therefore, determination of salivary antibodies against SARS-CoV-2 could be a non-invasive tool for disease surveillance, vaccination follow-up and to assist vaccination strategies against COVID-19.
Schlagwörter	covid19
Sprache	Englisch
Erscheinungsdatum	2022-12-16
Verlag	Cold Spring Harbor Laboratory Press
Dokumenttyp	Artikel ; Online
DOI	10.1101/2022.12.15.22283480
Datenquelle	COVID19

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Artikel: Humoral response to the BBIBP-CorV vaccine over time in healthcare workers with or without exposure to SARS-CoV-2

Badano, María Noel / Sabbione, Florencia / Keitelman, Irene / Pereson, Matias / Aloisi, Natalia / Colado, Ana / Ramos, María Victoria / Ortiz Wilczyñski, Juan Manuel / Pozner, Roberto Gabriel / Castillo, Luis / Wigdorovitz, Georgina / E.de Bracco, María Marta / Fink, Susana / Chuit, Roberto / Baré, Patricia

Molecular immunology. 2022 Mar., v. 143

2022

Abstract: SARS-CoV-2-specific humoral response was analyzed over time in a group of healthcare workers with or without exposure to SARS-CoV-2, who underwent vaccination with BBIBP-CorV (Sinopharm) vaccine in Argentina. Seroconversion rates in unexposed subjects ... ...

Abstract	SARS-CoV-2-specific humoral response was analyzed over time in a group of healthcare workers with or without exposure to SARS-CoV-2, who underwent vaccination with BBIBP-CorV (Sinopharm) vaccine in Argentina. Seroconversion rates in unexposed subjects after the first and second doses were 40 % and 100 %, respectively, showing a significant increase in antibody concentrations from dose 1 to dose 2 (p < 0.0001). The highest antibody concentrations were found in younger subjects and women, remaining significantly associated in a multivariable linear regression model (p = 0.005). A single dose of the BBIBP-CorV vaccine induced a strong antibody response in individuals with prior SARS-CoV-2infection, while a second dose did not increase this response. A sharp increase in antibody concentrations was observed following SARS-CoV-2 infection in those participants who became infected after the first and second doses (p = 0.008). Individuals with SARS-CoV-2 exposure prior to vaccination showed significantly higher anti-spike IgG antibody levels, at all-time points, than those not exposed (p < 0.001). Higher antibody titers were induced by a single dose in previously SARS-CoV-2 infected individuals than those induced in naïve subjects by two doses of the vaccine (p < 0.0001). Three months after the second dose both groups showed a decline in antibody levels, being more abrupt in unexposed subjects. Overall, our results showed a trend towards lower antibody concentrations over time following BBIBP-CorV vaccination. Sex and age seem to influence the magnitude of the humoral response in unexposed subjects while the combination of exposure to SARS-CoV-2 plus vaccination, whatever the sequence of the events was, produced a sharp increase in antibody levels. Evaluation of the humoral responses over time and the analysis of the induction and persistence of memory B and T cell responses, are needed to assess long-term immune protection induced by BBIBP-CorV vaccine.
Schlagwörter	Severe acute respiratory syndrome coronavirus 2 ; T-lymphocytes ; antibodies ; antibody formation ; health services ; humoral immunity ; memory ; regression analysis ; seroconversion ; vaccination ; vaccines ; Argentina
Sprache	Englisch
Erscheinungsverlauf	2022-03
Umfang	p. 94-99.
Erscheinungsort	Elsevier Ltd
Dokumenttyp	Artikel
ZDB-ID	424427-8
ISSN	1872-9142 ; 0161-5890
ISSN (online)	1872-9142
ISSN	0161-5890
DOI	10.1016/j.molimm.2022.01.009
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: A multidisciplinary, collaborative, inter-agency and comprehensive approach for the control of Chagas Disease as a public health problem in Guatemala

Monroy, María Carlota / Penados, Daniel / Pineda, José / Ruiz, Elisa Laparra / Agreda, Emmanuel O. / Alcantara, Belter / Rodas, Antonieta / Lange, Karla / Weinberg, Diego / Bazzani, Roberto / Marchiol, Andrea / Herazo, Rafael / Agrelo, Roberto Salvatella / Abril, Marcelo / Chuit, Roberto

Acta tropica. 2022 Jan., v. 225

2022

Abstract: The Pan American Health Organization (PAHO) has defined Chagas Disease hotspots in Central America associated with the vector Triatoma spp. Triatoma dimidiata is a native vector adapted to multiple environments, including intra-domestic and peri-domestic ...

Abstract	The Pan American Health Organization (PAHO) has defined Chagas Disease hotspots in Central America associated with the vector Triatoma spp. Triatoma dimidiata is a native vector adapted to multiple environments, including intra-domestic and peri-domestic habitats. A multi-institutional project named “Alliances for the elimination of Chagas in Central America” was created to help reduce the incidence of the disease in the region. Activities performed in the field as part of the project included aspects of vector surveillance and control, improvement of houses, diagnosis and treatment of individuals, health promotion, training of human resources and identification of access barriers to diagnosis and treatment. As a base line study, eleven villages, comprised of 1,572 households, were entomologically evaluated (83.4% overall participation); five were found to have very high infestation rates (>20%), three had high infestation rates (8-20%) and three had low-infestation rates (<8%), coinciding with the category of infestation-risk of the houses within each village. Serological tests were carried out in 812 people (>80% participation) in two of the 11 villages and none of the 128 children tested, less than 5 years of age, were positive for Trypanosoma cruzi infection. Community participation in all the activities was high (>70%). The collaboration between several subnational, national, and international institutions, each with specific roles, promoted community participation in the activities of vector control and patient care, thus, establishing a baseline to continue implementing and monitoring project progress.
Schlagwörter	Chagas disease ; Triatoma dimidiata ; Trypanosoma cruzi ; community service ; health promotion ; humans ; monitoring ; patient care ; people ; vector control ; villages ; Guatemala
Sprache	Englisch
Erscheinungsverlauf	2022-01
Erscheinungsort	Elsevier B.V.
Dokumenttyp	Artikel
ZDB-ID	210415-5
ISSN	1873-6254 ; 0001-706X
ISSN (online)	1873-6254
ISSN	0001-706X
DOI	10.1016/j.actatropica.2021.106157
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: Impact of a public health intervention for active surveillance and mitigation of SARS-CoV-2 in a district from Buenos Aires province, Argentina: a descriptive epidemiological study.

Crudo, Favio / Fernández, Mariana / Rodríguez Fermepín, Marcelo / Entrocassi, Andrea Carolina / Cardone, Karina Angela / Spina Markmann, Fernando / Periago, Maria Victoria / Chuit, Roberto / Abril, Marcelo Claudio

BMJ open

2021 Band 11, Heft 11, Seite(n) e053595

Abstract: Background: The first case of SARS-CoV-2 was reported in Argentina on 3 March 2020. Measures to limit the spread of the virus were implemented, including complete lockdown (26 March). Nonetheless, the virus spread throughout the country, with a first ... ...

Abstract	Background: The first case of SARS-CoV-2 was reported in Argentina on 3 March 2020. Measures to limit the spread of the virus were implemented, including complete lockdown (26 March). Nonetheless, the virus spread throughout the country, with a first peak of almost a million cases in October. On 30 November, the government's recommendation switched from social, preventive and compulsory isolation, to social, preventive and compulsory distancing. Objectives: To describe a tailored public health strategy to mitigate the spread of SARS-CoV-2 and determine its behaviour in San Antonio de Areco district from Buenos Aires province (Argentina) through a private-public association. Design, setting and participants: Surveillance of the virus was performed with the local healthcare system, through early identification of cases and the systematic study of each infected individual and contact, regardless of symptomatology, using telemedicine and a COVID-19-specific outpatient clinic. Real-time PCR was used for detection using both individual and pooled samples, with a 12-hour turnaround time. Results: Up to 30 November, a total of 2426 suspected cases were analysed and 578 were confirmed. Surveillance of health personnel and at-risk populations proved effective, mitigating viral spread. Pooling samples allowed reduction of operator time, helped reduce costs, and allowed detection of both symptomatic and asymptomatic cases. Conclusion: After 8 months of protocol implementation, the strategy to intensively survey groups at higher epidemiological risk and the systematic search for asymptomatic cases with the incorporation of pooled PCR for diagnosis, in combination with individual testing, is an efficient and viable option in populations with similar characteristics, in the frame of social isolation.
Mesh-Begriff(e)	Argentina/epidemiology ; COVID-19 ; Communicable Disease Control ; Delivery of Health Care ; Epidemiologic Studies ; Humans ; Public Health ; SARS-CoV-2 ; Watchful Waiting
Sprache	Englisch
Erscheinungsdatum	2021-11-26
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2021-053595
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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