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  1. Artikel ; Online: Inflammation, Autoinflammation and Autoimmunity in Inflammatory Bowel Diseases.

    Padoan, Andrea / Musso, Giulia / Contran, Nicole / Basso, Daniela

    Current issues in molecular biology

    2023  Band 45, Heft 7, Seite(n) 5534–5557

    Abstract: In this review, the role of innate and adaptive immunity in the pathogenesis of inflammatory bowel diseases (IBD) is reported. In IBD, an altered innate immunity is often found, with increased Th17 and decreased Treg cells infiltrating the intestinal ... ...

    Abstract In this review, the role of innate and adaptive immunity in the pathogenesis of inflammatory bowel diseases (IBD) is reported. In IBD, an altered innate immunity is often found, with increased Th17 and decreased Treg cells infiltrating the intestinal mucosa. An associated increase in inflammatory cytokines, such as IL-1 and TNF-α, and a decrease in anti-inflammatory cytokines, such as IL-10, concur in favoring the persistent inflammation of the gut mucosa. Autoinflammation is highlighted with insights in the role of inflammasomes, which activation by exogenous or endogenous triggers might be favored by mutations of NOD and NLRP proteins. Autoimmunity mechanisms also take place in IBD pathogenesis and in this context of a persistent immune stimulation by bacterial antigens and antigens derived from intestinal cells degradation, the adaptive immune response takes place and results in antibodies and autoantibodies production, a frequent finding in these diseases. Inflammation, autoinflammation and autoimmunity concur in altering the mucus layer and enhancing intestinal permeability, which sustains the vicious cycle of further mucosal inflammation.
    Sprache Englisch
    Erscheinungsdatum 2023-06-30
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2000024-8
    ISSN 1467-3045 ; 1467-3037
    ISSN (online) 1467-3045
    ISSN 1467-3037
    DOI 10.3390/cimb45070350
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Vitamin D Prevents Pancreatic Cancer-Induced Apoptosis Signaling of Inflammatory Cells.

    Moz, Stefania / Contran, Nicole / Facco, Monica / Trimarco, Valentina / Plebani, Mario / Basso, Daniela

    Biomolecules

    2020  Band 10, Heft 7

    Abstract: Combined approaches based on immunotherapy and drugs supporting immune effector cell function might increase treatment options for pancreatic ductal adenocarcinoma (PDAC), vitamin D being a suitable drug candidate. In this study, we evaluated whether ... ...

    Abstract Combined approaches based on immunotherapy and drugs supporting immune effector cell function might increase treatment options for pancreatic ductal adenocarcinoma (PDAC), vitamin D being a suitable drug candidate. In this study, we evaluated whether treatment with the vitamin D analogue, calcipotriol, counterbalances PDAC induced and SMAD4-associated intracellular calcium [Ca
    Mesh-Begriff(e) Calcitriol/analogs & derivatives ; Calcitriol/pharmacology ; Calcium/metabolism ; Carcinoma, Pancreatic Ductal/metabolism ; Cell Proliferation/drug effects ; Culture Media, Conditioned/chemistry ; Cytokines/metabolism ; Humans ; Lymphocytes/cytology ; Lymphocytes/drug effects ; Lymphocytes/metabolism ; NF-kappa B/metabolism ; Pancreatic Neoplasms/metabolism ; Signal Transduction/drug effects ; Smad4 Protein/metabolism ; Tumor Necrosis Factor-alpha/pharmacology ; Vitamin D/pharmacology
    Chemische Substanzen Culture Media, Conditioned ; Cytokines ; NF-kappa B ; SMAD4 protein, human ; Smad4 Protein ; Tumor Necrosis Factor-alpha ; Vitamin D (1406-16-2) ; calcipotriene (143NQ3779B) ; Calcitriol (FXC9231JVH) ; Calcium (SY7Q814VUP)
    Sprache Englisch
    Erscheinungsdatum 2020-07-15
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom10071055
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: New insights into SARS-CoV-2 Lumipulse G salivary antigen testing: accuracy, safety and short TAT enhance surveillance.

    Aita, Ada / Navaglia, Filippo / Moz, Stefania / Contran, Nicole / Barbaro, Francesco / Cattelan, Anna Maria / Padoan, Andrea / Cosma, Chiara / Faggian, Diego / Plebani, Mario / Basso, Daniela

    Clinical chemistry and laboratory medicine

    2022  Band 61, Heft 2, Seite(n) 323–331

    Abstract: Objectives: The rapid, accurate and safe detection of SARS-CoV-2 is the key to improving surveillance and infection containment. The aim of the present study was to ascertain whether, after heat/chemical inactivation, SARS-CoV-2 N antigen ... ...

    Abstract Objectives: The rapid, accurate and safe detection of SARS-CoV-2 is the key to improving surveillance and infection containment. The aim of the present study was to ascertain whether, after heat/chemical inactivation, SARS-CoV-2 N antigen chemiluminescence (CLEIA) assay in saliva remains a valid alternative to molecular testing.
    Methods: In 2022, 139 COVID-19 inpatients and 467 healthcare workers were enrolled. In 606 self-collected saliva samples (Salivette), SARS-CoV-2 was detected by molecular (TaqPath rRT-PCR) and chemiluminescent Ag assays (Lumipulse G). The effect of sample pre-treatment (extraction solution-ES or heating) on antigen recovery was verified.
    Results: Salivary SARS-CoV-2 antigen assay was highly accurate (AUC=0.959, 95% CI: 0.943-0.974), with 90% sensitivity and 92% specificity. Of the 254 antigen positive samples, 29 were false positives. We demonstrated that heterophilic antibodies could be a cause of false positive results. A significant antigen concentration decrease was observed after ES treatment (p=0.0026), with misclassification of 43 samples. Heat had a minimal impact, after treatment the correct classification of cases was maintained.
    Conclusions: CLEIA SARS-CoV-2 salivary antigen provides accurate, timely and high-throughput results that remain accurate also after heat inactivation, thus ensuring a safer work environment. This supports the use of salivary antigen detection by CLEIA in surveillance programs.
    Mesh-Begriff(e) Humans ; SARS-CoV-2 ; COVID-19/diagnosis ; COVID-19 Testing ; Saliva ; Sensitivity and Specificity
    Sprache Englisch
    Erscheinungsdatum 2022-10-26
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 1418007-8
    ISSN 1437-4331 ; 1434-6621 ; 1437-8523
    ISSN (online) 1437-4331
    ISSN 1434-6621 ; 1437-8523
    DOI 10.1515/cclm-2022-0849
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 121: Hefte anzeigen Standort:
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  4. Artikel ; Online: Salivary proteomic analysis in asymptomatic and symptomatic SARS-CoV-2 infection: Innate immunity, taste perception and FABP5 proteins make the difference.

    Aita, Ada / Battisti, Ilaria / Contran, Nicole / Furlan, Serena / Padoan, Andrea / Franchin, Cinzia / Barbaro, Francesco / Cattelan, Anna Maria / Zambon, Carlo-Federico / Plebani, Mario / Basso, Daniela / Arrigoni, Giorgio

    Clinica chimica acta; international journal of clinical chemistry

    2022  Band 537, Seite(n) 26–37

    Abstract: Background and aim: SARS-CoV-2 infection spawns from an asymptomatic condition to a fatal disease. Age, comorbidities, and several blood biomarkers are associated with infection outcome. We searched for biomarkers by untargeted and targeted proteomic ... ...

    Abstract Background and aim: SARS-CoV-2 infection spawns from an asymptomatic condition to a fatal disease. Age, comorbidities, and several blood biomarkers are associated with infection outcome. We searched for biomarkers by untargeted and targeted proteomic analysis of saliva, a source of viral particles and host proteins.
    Methods: Saliva samples from 19 asymptomatic and 16 symptomatic SARS-CoV-2 infected subjects, and 20 controls were analyzed by LC-MS/MS for untargeted peptidomic (flow through of 10 kDa filter) and proteomic (trypsin digestion of filter retained proteins) profiling.
    Results: Peptides from 53 salivary proteins were identified. ADF was detected only in controls, while IL1RA only in infected subjects. PRPs, DSC2, FABP5, his-1, IL1RA, PRH1, STATH, SMR3B, ANXA1, MUC7, ACTN4, IGKV1-33 and TGM3 were significantly different between asymptomatic and symptomatic subjects. Retained proteins were 117, being 11 highly different between asymptomatic and symptomatic (fold change ≥2 or ≤-2). After validation by LC-MS/MS-SRM (selected reaction monitoring analysis), the most significant discriminant proteins at PCA were IL1RA, CYSTB, S100A8, S100A9, CA6, and FABP5.
    Conclusions: The differentially abundant proteins involved in innate immunity (S100 proteins), taste (CA6 and cystatins), and viral binding to the host (FABP5), appear to be of interest for use as potential biomarkers and drugs targets.
    Mesh-Begriff(e) Humans ; Proteomics ; Chromatography, Liquid ; COVID-19 ; Taste Perception ; SARS-CoV-2 ; Taste ; Tandem Mass Spectrometry ; Saliva/metabolism ; Biomarkers/metabolism ; Immunity, Innate ; Fatty Acid-Binding Proteins/metabolism ; Transglutaminases/metabolism
    Chemische Substanzen Biomarkers ; FABP5 protein, human ; Fatty Acid-Binding Proteins ; TGM3 protein, human (EC 2.3.2.13) ; Transglutaminases (EC 2.3.2.13) ; STATH protein, human
    Sprache Englisch
    Erscheinungsdatum 2022-10-10
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2022.09.023
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Peptidomic and proteomic analysis of stool for diagnosing IBD and deciphering disease pathogenesis.

    Basso, Daniela / Padoan, Andrea / D'Incà, Renata / Arrigoni, Giorgio / Scapellato, Maria Luisa / Contran, Nicole / Franchin, Cinzia / Lorenzon, Greta / Mescoli, Claudia / Moz, Stefania / Bozzato, Dania / Rugge, Massimo / Plebani, Mario

    Clinical chemistry and laboratory medicine

    2021  Band 58, Heft 6, Seite(n) 968–979

    Abstract: Background The sensitivities and specificities of C-reactive protein (CRP) and faecal calprotectin (fCal), as recommended for inflammatory bowel diseases (IBD) diagnosis and monitoring, are low. Our aim was to discover new stool protein/peptide ... ...

    Abstract Background The sensitivities and specificities of C-reactive protein (CRP) and faecal calprotectin (fCal), as recommended for inflammatory bowel diseases (IBD) diagnosis and monitoring, are low. Our aim was to discover new stool protein/peptide biomarkers for diagnosing IBD. Methods For peptides, MALDI-TOF/MS (m/z 1000-4000) was performed using stools from an exploratory (34 controls; 72 Crohn's disease [CD], 56 ulcerative colitis [UC]) and a validation (28 controls, 27 CD, 15 UC) cohort. For proteins, LTQ-Orbitrap XL MS analysis (6 controls, 5 CD, 5 UC) was performed. Results MALDI-TOF/MS spectra of IBD patients had numerous features, unlike controls. Overall, 426 features (67 control-associated, 359 IBD-associated) were identified. Spectra were classified as control or IBD (absence or presence of IBD-associated features). In the exploratory cohort, the sensitivity and specificity of this classification algorithm were 81% and 97%, respectively. Blind analysis of the validation cohort confirmed 97% specificity, with a lower sensitivity (55%) paralleling active disease frequency. Following binary logistic regression analysis, IBD was independently correlated with MALDI-TOF/MS spectra (p < 0.0001), outperforming fCal measurements (p = 0.029). The IBD-correlated m/z 1810.8 feature was a fragment of APC2, homologous with APC, over-expressed by infiltrating cells lining the surface in UC or the muscularis-mucosae in CD (assessed by immunohistochemistry). IBD-associated over-expressed proteins included immunoglobulins and neutrophil proteins, while those under-expressed comprised proteins of the nucleic acid assembly or those (OLFM4, ENPP7) related to cancer risk. Conclusions Our study provides evidence for the clinical utility of a novel proteomic method for diagnosing IBD and insight on the pathogenic role of APC. Moreover, the newly described IBD-associated proteins might become tools for cancer risk assessment in IBD patients.
    Mesh-Begriff(e) Adult ; Biomarkers/metabolism ; Cohort Studies ; Feces/chemistry ; Female ; Humans ; Inflammatory Bowel Diseases/diagnosis ; Inflammatory Bowel Diseases/etiology ; Inflammatory Bowel Diseases/metabolism ; Male ; Middle Aged ; Peptides/metabolism ; Proteomics ; Reproducibility of Results
    Chemische Substanzen Biomarkers ; Peptides
    Sprache Englisch
    Erscheinungsdatum 2021-08-02
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 1418007-8
    ISSN 1437-4331 ; 1434-6621 ; 1437-8523
    ISSN (online) 1437-4331
    ISSN 1434-6621 ; 1437-8523
    DOI 10.1515/cclm-2019-1125
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Salivary SARS-CoV-2 antigen rapid detection: A prospective cohort study.

    Basso, Daniela / Aita, Ada / Padoan, Andrea / Cosma, Chiara / Navaglia, Filippo / Moz, Stefania / Contran, Nicole / Zambon, Carlo-Federico / Maria Cattelan, Anna / Plebani, Mario

    Clinica chimica acta; international journal of clinical chemistry

    2021  Band 517, Seite(n) 54–59

    Abstract: Background and aim: SARS-CoV-2 quick testing is relevant for the containment of new pandemic waves. Antigen testing in self-collected saliva might be useful. We compared salivary and naso-pharyngeal swab (NPS) SARS-CoV-2 antigen detection by a rapid ... ...

    Abstract Background and aim: SARS-CoV-2 quick testing is relevant for the containment of new pandemic waves. Antigen testing in self-collected saliva might be useful. We compared salivary and naso-pharyngeal swab (NPS) SARS-CoV-2 antigen detection by a rapid chemiluminescent assay (CLEIA) and two different point-of-care (POC) immunochromatographic assays, with results of molecular testing.
    Methods: 234 patients were prospectively enrolled. Paired self-collected saliva (Salivette) and NPS were obtained to perform rRT-PCR, chemiluminescent (Lumipulse G) and POC (NPS: Fujirebio and Abbott; saliva: Fujirebio) for SARS-CoV-2 antigen detection.
    Results: The overall agreement between NPS and saliva rRT-PCR was 78.7%, reaching 91.7% at the first week from symptoms. SARS-CoV-2 CLEIA antigen was highly accurate in distinguishing positive and negative NPS (ROC-AUC = 0.939, 95%CI:0.903-0.977), with 81.6% sensitivity and 93.8% specificity. This assay on saliva reached the optimal value within 7 days from symptoms onset (Sensitivity: 72%; Specificity: 97%). Saliva POC antigen was limited in sensitivity (13%), performing better in NPS (Sensitivity: 48% and 66%; Specificity: 100% and 99% for Espline and Abbott respectively), depending on viral loads.
    Conclusions: Self-collected saliva is a valid alternative to NPS for SARS-CoV-2 detection by molecular, but also by CLEIA antigen testing, which is therefore potentially useful for large scale screening.
    Mesh-Begriff(e) Antigens, Viral/analysis ; COVID-19/diagnosis ; Humans ; Luminescent Measurements ; Nasopharynx/virology ; Pandemics ; Point-of-Care Testing ; Prospective Studies ; SARS-CoV-2 ; Saliva/chemistry ; Sensitivity and Specificity
    Chemische Substanzen Antigens, Viral
    Sprache Englisch
    Erscheinungsdatum 2021-02-21
    Erscheinungsland Netherlands
    Dokumenttyp Comparative Study ; Journal Article
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2021.02.014
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Salivary SARS-CoV-2 antigen rapid detection: a prospective cohort study

    Basso, Daniela / Aita, Ada / Padoan, Andrea / Cosma, Chiara / Navaglia, Filippo / Moz, Stefania / Contran, Nicole / Zambon, Carlo-Federico / Cattelan, Anna Maria / Plebani, Mario

    medRxiv

    Abstract: Background: SARS-CoV-2 quick testing and reporting are now considered relevant for the containment of new pandemic waves. Antigen testing in self-collected saliva might be useful. We compared the diagnostic performance of salivary and naso-pharyngeal ... ...

    Abstract Background: SARS-CoV-2 quick testing and reporting are now considered relevant for the containment of new pandemic waves. Antigen testing in self-collected saliva might be useful. We compared the diagnostic performance of salivary and naso-pharyngeal swab (NPS) SARS-CoV-2 antigen detection by a rapid chemiluminescent assay (CLEIA) and two different point-of-care (POC) immunochromatographic assays, with that of molecular testing. Methods. 234 patients were prospectively enrolled. Paired self-collected saliva (Salivette) and NPS were obtained to perform rRT-PCR, chemiluminescent (Lumipulse G) and POC (NPS: Fujirebio and Abbott; saliva: Fujirebio) for SARS-CoV-2 antigen detection. Results. The overall agreement between NPS and saliva rRT-PCR was 78.7%, reaching 91.7% at the first week from symptoms onset. SARS-CoV-2 CLEIA antigen was highly accurate in distinguishing between positive and negative NPS (ROC-AUC=0.939, 95%CI:0.903-0.977), with 81.6% sensitivity and 93.8% specificity. This assay on saliva had an overall good accuracy (ROC- AUC=0.805, 95%CI:0.740-0.870), reaching the optimal value within 7 days from symptom onset (Sensitivity: 72%; Specificity: 97%). POC antigen in saliva had a very limited sensitivity (13%), performing better in NPS (Sensitivity: 48% and 66%; Specificity: 100% and 99% for Espline and Abbott respectively), depending on viral loads. Conclusions: Self-collected saliva is a valid alternative to NPS for SARS-CoV-2 detection not only by molecular, but also by CLEIA antigen testing, for which the highest diagnostic accuracy was achieved in the first week from symptom onset. Saliva is not suitable for POC, although the accuracy of these tests appears satisfactory for NPS with high viral load.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-12-27
    Verlag Cold Spring Harbor Laboratory Press
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2020.12.24.20248825
    Datenquelle COVID19

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