Artikel ; Online: Methods to Study Posttranslational Modification Patterns in Cytotoxic T-Cells and Cancer.
Methods in molecular biology (Clifton, N.J.)
2021 Band 2325, Seite(n) 137–153
Abstract: Protein posttranslational modifications (PTMs) regulate intracellular signaling associated with development and progression of many diseases; thus, they are key to understanding pathological mechanisms and set up more tailored therapies. In addition, ... ...
Abstract | Protein posttranslational modifications (PTMs) regulate intracellular signaling associated with development and progression of many diseases; thus, they are key to understanding pathological mechanisms and set up more tailored therapies. In addition, many posttranslationally modified proteins are released into biological fluids and can be used as new and more specific biomarkers. Based on this evidence, we analyzed the role of some PTMs in cancer and described the correlation between specific PTMs and T-cells activation/inhibition in cancer microenvironment. In the second part of this chapter, we analyzed the most commonly used approaches for qualitative and quantitative determination of PTMs. The comparison of three distinct but often complementary methodologies such as immunoblotting, mass spectrometry, and ELISA assays has allowed to highlight the pros and cons of each approach with a focus on their current application and their future developments to obtain more confident biomarkers and therapeutic targets useful for diagnosis, prognosis, and monitoring of the response to therapy. |
---|---|
Mesh-Begriff(e) | Biomarkers, Tumor/metabolism ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Immunoblotting/methods ; Lymphocyte Activation/immunology ; Mass Spectrometry/methods ; Neoplasms/immunology ; Neoplasms/metabolism ; Protein Processing, Post-Translational ; Proteomics/methods ; T-Lymphocytes, Cytotoxic/metabolism ; Tumor Microenvironment/immunology |
Chemische Substanzen | Biomarkers, Tumor |
Sprache | Englisch |
Erscheinungsdatum | 2021-05-29 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-1507-2_10 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.
Fernleihe an ZB MED
Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.