Artikel ; Online: Novel method for detecting complement C3 deposition on Staphylococcus aureus.
2022 Band 12, Heft 1, Seite(n) 15766
Abstract: The primary host response to Staphylococcus aureus infection occurs via complement. Complement is an elegant evolutionarily conserved system, playing essential roles in early defences by working in concert with immune cells to survey, label and destroy ... ...
Abstract | The primary host response to Staphylococcus aureus infection occurs via complement. Complement is an elegant evolutionarily conserved system, playing essential roles in early defences by working in concert with immune cells to survey, label and destroy microbial intruders and coordinate inflammation. Currently the exact mechanisms employed by S. aureus to manipulate and evade complement is not clear and is hindered by the lack of accurate molecular tools that can report on complement deposition on the bacterial surface. Current gold-standard detection methods employ labelled complement-specific antibodies and flow cytometry to determine complement deposited on bacteria. These methods are restricted by virtue of the expression of the S. aureus immunoglobulin binding proteins, Protein A and Sbi. In this study we describe the use of a novel antibody-independent C3 probe derived from the staphylococcal Sbi protein, specifically Sbi-IV domain. Here we show that biotin-labelled Sbi-IV interacts specifically with deposited C3 products on the staphylococcal surface and thus can be used to measure complement fixation on wild-type cells expressing a full repertoire of immune evasion proteins. Lastly, our data indicates that genetically diverse S. aureus strains restrict complement to different degrees suggesting that complement evasion is a variable virulence trait among S. aureus isolates. |
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Mesh-Begriff(e) | Bacterial Proteins ; Biotin/metabolism ; Complement C3/metabolism ; Humans ; Protein Binding ; Staphylococcal Infections ; Staphylococcus aureus/metabolism |
Chemische Substanzen | Bacterial Proteins ; Complement C3 ; Biotin (6SO6U10H04) |
Sprache | Englisch |
Erscheinungsdatum | 2022-09-21 |
Erscheinungsland | England |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2615211-3 |
ISSN | 2045-2322 ; 2045-2322 |
ISSN (online) | 2045-2322 |
ISSN | 2045-2322 |
DOI | 10.1038/s41598-022-20098-7 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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