Artikel ; Online: Acute Myelomonoblastic Leukemia (My1/De): A Preclinical Rat Model.
2024 Band 38, Heft 3, Seite(n) 1064–1073
Abstract: Background/aim: Since acute myeloid leukemias still represent the most aggressive type of adult acute leukemias, the profound understanding of disease pathology is of paramount importance for diagnostic and therapeutic purposes. Hence, this study aimed ... ...
| Abstract | Background/aim: Since acute myeloid leukemias still represent the most aggressive type of adult acute leukemias, the profound understanding of disease pathology is of paramount importance for diagnostic and therapeutic purposes. Hence, this study aimed to explore the real-time disease fate with the establishment of an experimental myelomonoblastic leukemia (My1/De) rat model using preclinical positron emission tomography (PET) and whole-body autoradiography. Materials and methods: In vitro [ Results: The tracer accumulation in the My1/De culture cells exceeded that of both the tumorous and the healthy bone marrow suspensions (p<0.01). Based on in vivo imaging, the subrenally transplanted My1/De cells resulted in the development of leukemia in the abdominal organs, and metastasized to the mesenterial and thoracic parathymic lymph nodes (PTLNs). The lymphatic spread of metastasis was further confirmed by the significantly higher %ID/g values of the metastatic PTLNs (4.25±0.28) compared to the control (0.94±0.34). Cytochemical staining of the peripheral blood, autopsy findings, and wright-Giemsa-stained post-mortem histological sections proved the leukemic involvement of the assessed tissues/organs. Conclusion: The currently established My1/De model appears to be well-suited for further leukemia-related therapeutic and diagnostic investigations. |
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| Mesh-Begriff(e) | Animals ; Rats ; Disease Models, Animal ; Fluorodeoxyglucose F18 ; Autoradiography ; Positron-Emission Tomography ; Cell Line, Tumor ; Tissue Distribution ; Leukemia, Myeloid, Acute/pathology ; Leukemia, Myeloid, Acute/metabolism ; Leukemia, Myeloid, Acute/diagnostic imaging ; Radiopharmaceuticals ; Male ; Humans |
| Chemische Substanzen | Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Radiopharmaceuticals |
| Sprache | Englisch |
| Erscheinungsdatum | 2024-04-28 |
| Erscheinungsland | Greece |
| Dokumenttyp | Journal Article |
| ZDB-ID | 807031-3 |
| ISSN | 1791-7549 ; 0258-851X |
| ISSN (online) | 1791-7549 |
| ISSN | 0258-851X |
| DOI | 10.21873/invivo.13540 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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