Article ; Online: Response surface methodological optimization of L-asparaginase production from the medicinal plant endophyte Acinetobacter baumannii ZAS1.
Journal, genetic engineering & biotechnology
2022 Volume 20, Issue 1, Page(s) 22
Abstract: Background: This study targets the enhanced production of L-asparaginase, an antitumor enzyme by Acinetobacter baumannii ZAS1. This organism is an endophyte isolated from the medicinal plant Annona muricata. Plackett-Burman design (PBD) and central ... ...
| Abstract | Background: This study targets the enhanced production of L-asparaginase, an antitumor enzyme by Acinetobacter baumannii ZAS1. This organism is an endophyte isolated from the medicinal plant Annona muricata. Plackett-Burman design (PBD) and central composite design (CCD) were used for statistical optimization of media components. Results: The organism exhibited 18.85 ± 0.2 U/mL enzyme activities in unoptimized media. Eight variables: L-asparagine, peptone, glucose, lactose, yeast extract, NaCl, MgSO Conclusion: The findings of this study indicated that an endophyte, Acinetobacter baumannii ZAS1 that produces L-asparaginase could be used to increase enzyme output. However, using the statistical methods Plackett-Burman design and central composite design of response surface methodology is a handy tool for optimizing media components for increased L-asparaginase synthesis. |
|---|---|
| Language | English |
| Publishing date | 2022-02-09 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 2637420-1 |
| ISSN | 2090-5920 ; 1687-157X ; 2090-5920 |
| ISSN (online) | 2090-5920 |
| ISSN | 1687-157X ; 2090-5920 |
| DOI | 10.1186/s43141-022-00309-4 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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