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  1. Artikel ; Online: TLR7 in channel catfish (Ictalurus punctatus) is expressed in the endolysosome and is stimulated by synthetic ssRNA analogs, imiquimod, and resiquimod.

    Felch, Kristianna L / Crider, Jonathan D / Bhattacharjee, Debduti / Huhn, Cameron / Wilson, Melanie / Bengtén, Eva

    Developmental and comparative immunology

    2024  Band 157, Seite(n) 105197

    Abstract: Toll-like receptors (TLRs) are pivotal pattern recognition receptors (PRRs) and key mediators of innate immunity. Despite the significance of channel catfish (Ictalurus punctatus) in comparative immunology and aquaculture, its 20 TLR genes remain largely ...

    Abstract Toll-like receptors (TLRs) are pivotal pattern recognition receptors (PRRs) and key mediators of innate immunity. Despite the significance of channel catfish (Ictalurus punctatus) in comparative immunology and aquaculture, its 20 TLR genes remain largely functionally uncharacterized. In this study, our aim was to determine the catfish TLR7 agonists, signaling potential, and cellular localization. Using a mammalian reporter system, we identified imiquimod and resiquimod, typical ssRNA analogs, as potent catfish TLR7 agonists. Notably, unlike grass carp TLR7, catfish TLR7 lacks the ability to respond to poly (I:C). Confocal microscopy revealed predominant catfish TLR7 expression in lysosomes, co-localizing with the endosomal chaperone protein, UNC93B1. Furthermore, imiquimod stimulation elicited robust IFNb transcription in peripheral blood leukocytes isolated from adult catfish. These findings underscore the conservation of TLR7 signaling in catfish, reminiscent of mammalian TLR7 responses. Our study sheds light on the functional aspects of catfish TLR7 and contributes to a better understanding of its role in immune defense mechanisms.
    Mesh-Begriff(e) Animals ; Imiquimod ; Toll-Like Receptor 7/metabolism ; Toll-Like Receptor 7/agonists ; Toll-Like Receptor 7/genetics ; Imidazoles/pharmacology ; Ictaluridae/immunology ; Lysosomes/metabolism ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Immunity, Innate ; Signal Transduction ; Humans ; Aminoquinolines/pharmacology ; Poly I-C/immunology
    Chemische Substanzen Imiquimod (P1QW714R7M) ; Toll-Like Receptor 7 ; resiquimod (V3DMU7PVXF) ; Imidazoles ; Fish Proteins ; Aminoquinolines ; Poly I-C (O84C90HH2L)
    Sprache Englisch
    Erscheinungsdatum 2024-05-17
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 752411-0
    ISSN 1879-0089 ; 0145-305X
    ISSN (online) 1879-0089
    ISSN 0145-305X
    DOI 10.1016/j.dci.2024.105197
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: A subset of leukocyte immune-type receptors (LITRs) regulates phagocytosis in channel catfish (Ictalurus punctatus) leukocytes

    Crider, Jonathan / Wilson, Melanie / Felch, Kristianna L. / Dupre, Rebecca A. / Quiniou, Sylvie M.A. / Bengtén, Eva

    Molecular Immunology. 2023 Feb., v. 154 p.33-44

    2023  

    Abstract: Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; ...

    Abstract Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; however, LITR-mediated phagocytosis has not been examined in the catfish model. In this study, we use two anti-LITR monoclonal antibodies, CC41 and 125.2, to contrast the effects of crosslinking subsets of inhibitory and activating LITRs. Briefly, LITRs expressed by catfish γδ T cells, αβ T cells, and macrophage cell lines were crosslinked using mAb-conjugated fluorescent microbeads, and bead uptake was evaluated by flow cytometry and confirmed by confocal microscopy. A clear difference in the uptake of 125.2- and CC41-conjugated beads was observed. Crosslinking LITRs with mAb 125.2 resulted in efficient bead internalization, while mAb CC41 crosslinking of inhibitory LITRs resulted predominantly in a capturing phenotype. Pretreating catfish macrophages with mAb CC41 resulted in a marked decrease in LITR-mediated phagocytosis of 125.2-conjugated beads. Overall, these findings provide insight into fish immunobiology and validate LITRs as regulators of phagocytosis in catfish macrophages and γδ T cells.
    Schlagwörter Ictalurus punctatus ; catfish ; confocal microscopy ; crosslinking ; flow cytometry ; fluorescence ; immunomodulation ; leukocytes ; macrophages ; mammals ; microbeads ; models ; phagocytosis ; phenotype ; APC ; μME ; BSA ; cfPBS ; cfRPMI ; CCV ; CEACAM ; Csk ; CTL ; CYT ; DAP10 ; DAP12 ; DICP ; FcR ; FcRL ; HA ; Ig ; IgSF ; ITAM ; ITIM ; ITSM ; KIR ; LAK ; LILR ; LITR ; MHC ; MLC ; nanoLC-MS/MS ; Nck ; NILT ; NITR ; NK ; NKG2D ; PBL ; PIGRL ; RBL ; TM ; TREM ; SHP-2 ; Siglec ; SIRP ; WGA ; Paired receptors ; Immunoregulatory receptors ; Inhibitory signaling ; Cytoplasmic tails ; Innate immunity ; Leukocyte immune-type receptors ; Channel catfish
    Sprache Englisch
    Erscheinungsverlauf 2023-02
    Umfang p. 33-44.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel ; Online
    Anmerkung Use and reproduction
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2022.12.009
    Datenquelle NAL Katalog (AGRICOLA)

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    ab Jg. 2022: Lesesaal (EG)

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  3. Artikel ; Online: Interferons and interferon receptors in the channel catfish, Ictalurus punctatus

    Quiniou, Sylvie M.A. / Crider, Jonathan / Felch, Kristianna L. / Bengtén, Eva / Boudinot, Pierre

    Fish and Shellfish Immunology. 2022 Apr., v. 123 p.442-452

    2022  

    Abstract: In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups ...

    Abstract In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups previously defined in salmonids (a-f.) No representatives of subgroup h previously only found in percomorphs were identified. An expansion in copy numbers of subgroup d IFN genes was of particular interest, as this has not been reported in other fish species to date. Furthermore, we confirmed the presence of two type II ifn genes encoding orthologs of IFNγ and the teleost-specific IFNγRel. Six homologs of IFN type I receptor genes were found in an array that shows conserved synteny with human chromosome 21. Three homologs of type II IFN receptor genes were also identified. These type I and type II receptor sequences are compatible with the dual type I IFN receptors, and the potentially more complex type II IFN receptors described in teleosts. Our data provide a comprehensive resource for future studies of channel catfish innate antiviral immunity.
    Schlagwörter Ictalurus punctatus ; Salmonidae ; chromosomes ; fish ; genomics ; humans ; immunity ; interferons ; shellfish ; transcriptomics ; Interferon ; Antiviral immunity ; Teleost ; Inflammation ; Innate immunity ; Channelcatfish
    Sprache Englisch
    Erscheinungsverlauf 2022-04
    Umfang p. 442-452.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel ; Online
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2022.02.019
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  4. Artikel ; Online: A subset of leukocyte immune-type receptors (LITRs) regulates phagocytosis in channel catfish (Ictalurus punctatus) leukocytes.

    Crider, Jonathan / Wilson, Melanie / Felch, Kristianna L / Dupre, Rebecca A / Quiniou, Sylvie M A / Bengtén, Eva

    Molecular immunology

    2022  Band 154, Seite(n) 33–44

    Abstract: Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; ...

    Abstract Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; however, LITR-mediated phagocytosis has not been examined in the catfish model. In this study, we use two anti-LITR monoclonal antibodies, CC41 and 125.2, to contrast the effects of crosslinking subsets of inhibitory and activating LITRs. Briefly, LITRs expressed by catfish γδ T cells, αβ T cells, and macrophage cell lines were crosslinked using mAb-conjugated fluorescent microbeads, and bead uptake was evaluated by flow cytometry and confirmed by confocal microscopy. A clear difference in the uptake of 125.2- and CC41-conjugated beads was observed. Crosslinking LITRs with mAb 125.2 resulted in efficient bead internalization, while mAb CC41 crosslinking of inhibitory LITRs resulted predominantly in a capturing phenotype. Pretreating catfish macrophages with mAb CC41 resulted in a marked decrease in LITR-mediated phagocytosis of 125.2-conjugated beads. Overall, these findings provide insight into fish immunobiology and validate LITRs as regulators of phagocytosis in catfish macrophages and γδ T cells.
    Mesh-Begriff(e) Animals ; Ictaluridae/genetics ; Ictaluridae/metabolism ; Receptors, Immunologic ; Phagocytosis ; Leukocytes ; Catfishes ; Mammals
    Chemische Substanzen Receptors, Immunologic
    Sprache Englisch
    Erscheinungsdatum 2022-12-29
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2022.12.009
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 166: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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    ab Jg. 2022: Lesesaal (EG)

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  5. Artikel ; Online: Interferons and interferon receptors in the channel catfish, Ictalurus punctatus.

    Quiniou, Sylvie M A / Crider, Jonathan / Felch, Kristianna L / Bengtén, Eva / Boudinot, Pierre

    Fish & shellfish immunology

    2022  Band 123, Seite(n) 442–452

    Abstract: In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups ...

    Abstract In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups previously defined in salmonids (a-f.) No representatives of subgroup h previously only found in percomorphs were identified. An expansion in copy numbers of subgroup d IFN genes was of particular interest, as this has not been reported in other fish species to date. Furthermore, we confirmed the presence of two type II ifn genes encoding orthologs of IFNγ and the teleost-specific IFNγRel. Six homologs of IFN type I receptor genes were found in an array that shows conserved synteny with human chromosome 21. Three homologs of type II IFN receptor genes were also identified. These type I and type II receptor sequences are compatible with the dual type I IFN receptors, and the potentially more complex type II IFN receptors described in teleosts. Our data provide a comprehensive resource for future studies of channel catfish innate antiviral immunity.
    Mesh-Begriff(e) Animals ; Genome ; Ictaluridae/genetics ; Interferons/genetics ; Phylogeny ; Receptors, Interferon/genetics
    Chemische Substanzen Receptors, Interferon ; Interferons (9008-11-1)
    Sprache Englisch
    Erscheinungsdatum 2022-03-15
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2022.02.019
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: A Comprehensive Annotation of the Channel Catfish (

    Crider, Jonathan / Quiniou, Sylvie M A / Felch, Kristianna L / Showmaker, Kurt / Bengtén, Eva / Wilson, Melanie

    Frontiers in immunology

    2021  Band 12, Seite(n) 786402

    Abstract: The complete germline repertoires of the channel catfish, ...

    Abstract The complete germline repertoires of the channel catfish,
    Mesh-Begriff(e) Animals ; Evolution, Molecular ; Fish Proteins/genetics ; Fish Proteins/immunology ; Genes, T-Cell Receptor ; Genes, T-Cell Receptor alpha ; Genes, T-Cell Receptor beta ; Genes, T-Cell Receptor delta ; Genes, T-Cell Receptor gamma ; Genetic Loci ; Ictaluridae/genetics ; Ictaluridae/immunology ; Phylogeny ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/immunology ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Receptors, Antigen, T-Cell, alpha-beta/immunology ; Receptors, Antigen, T-Cell, gamma-delta/genetics ; Receptors, Antigen, T-Cell, gamma-delta/immunology ; Species Specificity ; Zebrafish/genetics ; Zebrafish/immunology ; Zebrafish Proteins/genetics ; Zebrafish Proteins/immunology
    Chemische Substanzen Fish Proteins ; Receptors, Antigen, T-Cell ; Receptors, Antigen, T-Cell, alpha-beta ; Receptors, Antigen, T-Cell, gamma-delta ; Zebrafish Proteins
    Sprache Englisch
    Erscheinungsdatum 2021-11-25
    Erscheinungsland Switzerland
    Dokumenttyp Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.786402
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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