Artikel: A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata
Developmental and comparative immunology. 2018 Feb., v. 79
2018
Abstract: Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of- ... ...
Abstract | Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-κB pathway mobilization. Biochemical and cell-based assays revealed that Of-NF-κB resembles the mammalian non-canonical NF-κB protein p100 in that C-terminal truncation results in translocation of Of-NF-κB to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human IκB kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-κB in vitro and induce C-terminal processing of Of-NF-κB in vivo. These results are the first characterization of TLR-to-NF-κB signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways. |
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Schlagwörter | coral reefs ; corals ; humans ; IKappaB kinase ; ligands ; lipopolysaccharides ; signal transduction ; Toll-like receptor 4 ; transcription factor NF-kappa B ; transcriptional activation | |||||
Sprache | Englisch | |||||
Erscheinungsverlauf | 2018-02 | |||||
Umfang | p. 128-136. | |||||
Erscheinungsort | Elsevier Ltd | |||||
Dokumenttyp | Artikel | |||||
ZDB-ID | 752411-0 | |||||
ISSN | 1879-0089 ; 0145-305X | |||||
ISSN (online) | 1879-0089 | |||||
ISSN | 0145-305X | |||||
DOI | 10.1016/j.dci.2017.10.016 | |||||
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Datenquelle | NAL Katalog (AGRICOLA) |
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