Artikel ; Online: Protein-Mediated Changes in Membrane Fluidity and Ordering: Insights into the Molecular Mechanism and Implications for Cellular Function.
The journal of physical chemistry letters
2024 Band 15, Heft 16, Seite(n) 4408–4415
Abstract: Probing protein-membrane interactions is vital for understanding biological functionality for various applications such as drug development, targeted drug delivery, and creation of functional biomaterials for medical and industrial purposes. In this ... ...
Abstract | Probing protein-membrane interactions is vital for understanding biological functionality for various applications such as drug development, targeted drug delivery, and creation of functional biomaterials for medical and industrial purposes. In this study, we have investigated interaction of Human Serum Albumin (HSA) with two different lipids, dipalmitoylphosphatidylglycerol (dDPPG) and dipalmitoylphosphatidylcholine (dDPPC), using Vibrational Sum Frequency Generation spectroscopy at different membrane fluidity values. In the liquid-expanded (LE) state of the lipid, HSA (at pH 3.5) deeply intercalated lipid chains through a combination of electrostatic and hydrophobic interactions, which resulted in more ordering of the lipid chains. However, in the liquid-condensed (LC) state, protein intercalation is decreased due to tighter lipid packing. Moreover, our findings revealed distinct differences in HSA's interaction with dDPPG and dDPPC lipids. The interaction with dDPPC remained relatively weak compared to dDPPG. These results shed light on the significance of protein mediated changes in lipid characteristics, which hold considerable implications for understanding membrane protein behavior, lipid-mediated cellular processes, and lipid-based biomaterial design. |
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Mesh-Begriff(e) | Humans ; Phosphatidylglycerols/chemistry ; Phosphatidylglycerols/metabolism ; Membrane Fluidity ; 1,2-Dipalmitoylphosphatidylcholine/chemistry ; Hydrophobic and Hydrophilic Interactions ; Serum Albumin, Human/chemistry ; Serum Albumin, Human/metabolism ; Static Electricity |
Chemische Substanzen | Phosphatidylglycerols ; 1,2-Dipalmitoylphosphatidylcholine (2644-64-6) ; 1,2-dipalmitoylphosphatidylglycerol (VA9U6BR3SB) ; Serum Albumin, Human (ZIF514RVZR) |
Sprache | Englisch |
Erscheinungsdatum | 2024-04-16 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article |
ISSN | 1948-7185 |
ISSN (online) | 1948-7185 |
DOI | 10.1021/acs.jpclett.3c03627 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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