Article ; Online: Ex vivo model for elucidating the functional and structural differentiation of the embryonic mouse thyroid.
Molecular and cellular endocrinology
2020 Volume 510, Page(s) 110834
Abstract: Terminal thyroid gland differentiation - the last developmental step needed to enable thyroid hormone (T4) synthesis - involves profound structural and biochemical changes in the thyroid follicular cells (TFCs). We aimed to develop an ex vivo thyroid ... ...
Abstract | Terminal thyroid gland differentiation - the last developmental step needed to enable thyroid hormone (T4) synthesis - involves profound structural and biochemical changes in the thyroid follicular cells (TFCs). We aimed to develop an ex vivo thyroid model of embryonic mouse thyroid that would replicate the in vivo TFC differentiation program. E13.5 thyroid explants were cultured ex vivo in chemically defined medium for 7 days. Immunostaining and qPCR of thyroid explants showed thyroglobulin production onset, follicle formation, and T4 synthesis onset in 1-, 3-, and 5-day-old cultures, respectively. Differentiation was maintained and follicular growth continued throughout the 7-day culture period. Pharmacological approaches to culture inhibition were performed successfully in the ex vivo thyroids. Our robust and well described ex vivo thyroid culture model replicates the sequence of thyroid differentiation to T4 synthesis seen in vivo. This model can be used to test the effects of pharmacological inhibitors on thyroid hormone production. |
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MeSH term(s) | Animals ; Cell Differentiation ; Embryo, Mammalian/anatomy & histology ; Embryonic Development/genetics ; Gene Expression Regulation, Developmental ; Mice ; Microdissection ; Models, Biological ; Receptors, Notch/metabolism ; Thyroglobulin/metabolism ; Thyroid Gland/anatomy & histology ; Thyroid Gland/embryology ; Thyroid Gland/physiology ; Tissue Survival/physiology |
Chemical Substances | Receptors, Notch ; Thyroglobulin (9010-34-8) |
Language | English |
Publishing date | 2020-04-28 |
Publishing country | Ireland |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 187438-x |
ISSN | 1872-8057 ; 0303-7207 |
ISSN (online) | 1872-8057 |
ISSN | 0303-7207 |
DOI | 10.1016/j.mce.2020.110834 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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