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  1. Artikel ; Online: pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens.

    Parrish, Phoebe C R / Groso, Daniel J / Thomas, James D / Bradley, Robert K / Berger, Alice H

    ArXiv

    2023  

    Abstract: We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR ... ...

    Abstract We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples. pgMAP is implemented using Snakemake and is available open-source under the MIT license at https://github.com/fredhutch/pgmap_pipeline.
    Sprache Englisch
    Erscheinungsdatum 2023-06-01
    Erscheinungsland United States
    Dokumenttyp Preprint
    ISSN 2331-8422
    ISSN (online) 2331-8422
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Buch ; Online: pgMAP

    Parrish, Phoebe C. R. / Groso, Daniel J. / Thomas, James D. / Bradley, Robert K. / Berger, Alice H.

    a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens

    2023  

    Abstract: We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR ... ...

    Abstract We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples. pgMAP is implemented using Snakemake and is available open-source under the MIT license at https://github.com/fredhutch/pgmap_pipeline.

    Comment: 6 pages, 1 figure, code available at https://github.com/fredhutch/pgmap_pipeline
    Schlagwörter Quantitative Biology - Genomics
    Erscheinungsdatum 2023-06-01
    Erscheinungsland us
    Dokumenttyp Buch ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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