Artikel ; Online: Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs.
2023 Band 24, Heft 1, Seite(n) 770
Abstract: Background: As the largest substantive organ of animals, the liver plays an essential role in the physiological processes of digestive metabolism and immune defense. However, the cellular composition of the pig liver remains poorly understood. This ... ...
| Abstract | Background: As the largest substantive organ of animals, the liver plays an essential role in the physiological processes of digestive metabolism and immune defense. However, the cellular composition of the pig liver remains poorly understood. This investigation used single-nucleus RNA sequencing technology to identify cell types from liver tissues of pigs, providing a theoretical basis for further investigating liver cell types in pigs. Results: The analysis revealed 13 cells clusters which were further identified 7 cell types including endothelial cells, T cells, hepatocytes, Kupffer cells, stellate cells, B cells, and cholangiocytes. The dominant cell types were endothelial cells, T cells and hepatocytes in the liver tissue of Dahe pigs and Dahe black pigs, which accounts for about 85.76% and 82.74%, respectively. The number of endothelial cells was higher in the liver tissue of Dahe pigs compared to Dahe black pigs, while the opposite tendency was observed for T cells. Moreover, functional enrichment analysis demonstrated that the differentially expressed genes in pig hepatic endothelial cells were significantly enriched in the protein processing in endoplasmic reticulum, MAPK signaling pathway, and FoxO signaling pathway. Functional enrichment analysis demonstrated that the differentially expressed genes in pig hepatic T cells were significantly enriched in the thyroid hormone signaling pathway, B cell receptor signaling pathway, and focal adhesion. Functional enrichment analysis demonstrated that the differentially expressed genes in pig hepatic hepatocytes were significantly enriched in the metabolic pathways. Conclusions: In summary, this study provides a comprehensive cell atlas of porcine hepatic tissue. The number, gene expression level and functional characteristics of each cell type in pig liver tissue varied between breeds. |
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| Mesh-Begriff(e) | Animals ; Swine ; Transcriptome ; Endothelial Cells ; Plant Breeding ; Hepatocytes/metabolism ; Liver/metabolism |
| Sprache | Englisch |
| Erscheinungsdatum | 2023-12-12 |
| Erscheinungsland | England |
| Dokumenttyp | Journal Article |
| ZDB-ID | 2041499-7 |
| ISSN | 1471-2164 ; 1471-2164 |
| ISSN (online) | 1471-2164 |
| ISSN | 1471-2164 |
| DOI | 10.1186/s12864-023-09765-9 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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