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  1. Artikel: Genotyping of the Major SARS-CoV-2 Clade by Short-Amplicon High-Resolution Melting (SA-HRM) Analysis

    Diaz-Garcia, Hector / Guzmán-Ortiz, Ana L / Angeles-Floriano, Tania / Parra-Ortega, Israel / López-Martínez, Briceida / Martínez-Saucedo, Mirna / Aquino-Jarquin, Guillermo / Sánchez-Urbina, Rocío / Quezada, Hector / Granados-Riveron, Javier T

    Genes. 2021 Apr. 05, v. 12, no. 4

    2021  

    Abstract: The genome of the SARS-CoV-2 virus, the causal agent of the COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some mutations have defined several SARS-CoV-2 clades that seem to behave ... ...

    Abstract The genome of the SARS-CoV-2 virus, the causal agent of the COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some mutations have defined several SARS-CoV-2 clades that seem to behave differently in terms of regional distribution and other biological features. Next-generation sequencing (NGS) approaches are used to classify the sequence variants in viruses from individual human patients. However, the cost and relative scarcity of NGS equipment and expertise in developing countries prevent studies aimed to associate specific clades and variants to clinical features and outcomes in such territories. As of March 2021, the GR clade and its derivatives, including the B.1.1.7 and B.1.1.28 variants, predominate worldwide. We implemented the post-PCR small-amplicon high-resolution melting analysis to genotype SARS-CoV-2 viruses isolated from the saliva of individual patients. This procedure was able to clearly distinguish two groups of samples of SARS-CoV-2-positive samples predicted, according to their melting profiles, to contain GR and non-GR viruses. This grouping of the samples was validated by means of amplification-refractory mutation system (ARMS) assay as well as Sanger sequencing.
    Schlagwörter COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; animal pathogens ; equipment ; genotype ; genotyping ; humans ; mutation ; saliva ; viruses
    Sprache Englisch
    Erscheinungsverlauf 2021-0405
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12040531
    Datenquelle NAL Katalog (AGRICOLA)

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  2. Artikel ; Online: Genotyping of the Major SARS-CoV-2 Clade by Short-Amplicon High-Resolution Melting (SA-HRM) Analysis.

    Diaz-Garcia, Hector / Guzmán-Ortiz, Ana L / Angeles-Floriano, Tania / Parra-Ortega, Israel / López-Martínez, Briceida / Martínez-Saucedo, Mirna / Aquino-Jarquin, Guillermo / Sánchez-Urbina, Rocío / Quezada, Hector / Granados-Riveron, Javier T

    Genes

    2021  Band 12, Heft 4

    Abstract: The genome of the SARS-CoV-2 virus, the causal agent of the COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some mutations have defined several SARS-CoV-2 clades that seem to behave ... ...

    Abstract The genome of the SARS-CoV-2 virus, the causal agent of the COVID-19 pandemic, has diverged due to multiple mutations since its emergence as a human pathogen in December 2019. Some mutations have defined several SARS-CoV-2 clades that seem to behave differently in terms of regional distribution and other biological features. Next-generation sequencing (NGS) approaches are used to classify the sequence variants in viruses from individual human patients. However, the cost and relative scarcity of NGS equipment and expertise in developing countries prevent studies aimed to associate specific clades and variants to clinical features and outcomes in such territories. As of March 2021, the GR clade and its derivatives, including the B.1.1.7 and B.1.1.28 variants, predominate worldwide. We implemented the post-PCR small-amplicon high-resolution melting analysis to genotype SARS-CoV-2 viruses isolated from the saliva of individual patients. This procedure was able to clearly distinguish two groups of samples of SARS-CoV-2-positive samples predicted, according to their melting profiles, to contain GR and non-GR viruses. This grouping of the samples was validated by means of amplification-refractory mutation system (ARMS) assay as well as Sanger sequencing.
    Mesh-Begriff(e) COVID-19/virology ; Genotyping Techniques/methods ; High-Throughput Nucleotide Sequencing ; Humans ; Mutation ; Nucleic Acid Denaturation ; RNA, Viral/isolation & purification ; SARS-CoV-2/genetics
    Chemische Substanzen RNA, Viral
    Sprache Englisch
    Erscheinungsdatum 2021-04-05
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12040531
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Saliva as a promising biofluid for SARS-CoV-2 detection during the early stages of infection.

    López-Martínez, Briceida / Guzmán-Ortiz, Ana L / Nevárez-Ramírez, Abraham J / Parra-Ortega, Israel / Olivar-López, Víctor B / Ángeles-Floriano, Tania / Vilchis-Ordoñez, Armando / Quezada, Héctor

    Boletin medico del Hospital Infantil de Mexico

    2020  Band 77, Heft 5, Seite(n) 228–233

    Abstract: Background: Diagnostic testing for coronavirus disease (COVID)-19 is performed using nasopharyngeal swabs. This type of sampling is uncomfortable for the patient, dangerous for health workers, and its high demand has led to a global shortage of swabs. ... ...

    Titelübersetzung La saliva como biofluido promisorio para la detección del SARS-CoV-2 durante las primeras etapas de la infección.
    Abstract Background: Diagnostic testing for coronavirus disease (COVID)-19 is performed using nasopharyngeal swabs. This type of sampling is uncomfortable for the patient, dangerous for health workers, and its high demand has led to a global shortage of swabs. One of the alternative specimens is saliva. However, the optimal conditions for the test have not been established.
    Methods: Reverse transcription-polymerase chain reaction was used to detect the viral genome in saliva samples kept at room temperature, in the fridge or frozen for 2 days. In addition, the influence of brushing teeth and feeding on the detection of the virus in saliva was addressed. Finally, the efficiency of saliva in revealing the presence of the virus during the hospitalization period was determined in children.
    Results: The viral genome was consistently detected regardless of the storage conditions of saliva samples. Brushing teeth and feeding did not influence the sensitivity of the test. In hospitalized children, positive results were obtained only during the early days.
    Conclusions: These results support the idea of the use of saliva as an alternative specimen for diagnostic testing for COVID-19. The viral genome is stable and endures perturbations in the oral cavity. However, clearance of the virus from the mouth during the infection may limit the use of the test only to the early stages of the disease.
    Mesh-Begriff(e) Adolescent ; Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Child, Preschool ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/virology ; Female ; Genome, Viral ; Hospitalization ; Humans ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/virology ; Reverse Transcriptase Polymerase Chain Reaction/methods ; SARS-CoV-2 ; Saliva/virology ; Sensitivity and Specificity ; Specimen Handling/methods ; Temperature ; Time Factors
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-10-15
    Erscheinungsland Mexico
    Dokumenttyp Journal Article
    ZDB-ID 730519-9
    ISSN 1665-1146 ; 1665-1146 ; 0539-6115 ; 0539-6123
    ISSN (online) 1665-1146
    ISSN 1665-1146 ; 0539-6115 ; 0539-6123
    DOI 10.24875/BMHIM.20000204
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Extracellular Vesicles from Human Plasma Show a Distinctive Proteome and miRNome Profile in Patients with Severe Cutaneous Adverse Reactions.

    Salinas-Jaramillo, Orlando / Monroy-Arreola, Alejandra / Herrera-Noreña, Sebastian / Guzmán-Ortiz, Ana L / Hernández-Hernández, Abrahan / Méndez-Flores, Silvia / Domínguez-Cherit, Judith / Duran-Figueroa, Noe V / Naisbitt, Dean J / Cortes-Reynosa, Pedro / Perez Salazar, Eduardo / Quezada, Héctor / Castrejón-Flores, J Luis

    Chemical research in toxicology

    2021  Band 34, Heft 7, Seite(n) 1738–1748

    Abstract: Cutaneous drug-induced reactions are immune-mediated responses that can lead to life-threatening diseases such as drug reaction with eosinophilia and systemic symptoms (DRESS), Stevens-Johnson syndrome, and toxic epidermal necrolysis, collectively known ... ...

    Abstract Cutaneous drug-induced reactions are immune-mediated responses that can lead to life-threatening diseases such as drug reaction with eosinophilia and systemic symptoms (DRESS), Stevens-Johnson syndrome, and toxic epidermal necrolysis, collectively known as severe cutaneous adverse reactions (SCARs). Unfortunately, they cannot be predicted during drug development, and, at present, a prognostic biomarker is not available nor are validated
    Mesh-Begriff(e) Drug Eruptions/diagnosis ; Drug Eruptions/genetics ; Extracellular Vesicles/chemistry ; Extracellular Vesicles/genetics ; Extracellular Vesicles/pathology ; Humans ; MicroRNAs/genetics ; Proteome/analysis ; Proteome/genetics ; Proteomics ; Transcriptome
    Chemische Substanzen MicroRNAs ; Proteome
    Sprache Englisch
    Erscheinungsdatum 2021-06-18
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639353-6
    ISSN 1520-5010 ; 0893-228X
    ISSN (online) 1520-5010
    ISSN 0893-228X
    DOI 10.1021/acs.chemrestox.1c00047
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: La saliva como biofluido promisorio para la detección del SARS-CoV-2 durante las primeras etapas de la infección./ Saliva as a promising biofluid for SARS-CoV-2 detection during the early stages of infection

    López-Martínez, Briceida / Guzmán-Ortiz, Ana L / Nevárez-Ramírez, Abraham J / Parra-Ortega, Israel / Olivar-López, Víctor B / Ángeles-Floriano, Tania / Vilchis-Ordoñez, Armando / Quezada, Héctor

    Bol Med Hosp Infant Mex

    Abstract: Background: Diagnostic testing for coronavirus disease (COVID)-19 is performed using nasopharyngeal swabs. This type of sampling is uncomfortable for the patient, dangerous for health workers, and its high demand has led to a global shortage of swabs. ... ...

    Abstract Background: Diagnostic testing for coronavirus disease (COVID)-19 is performed using nasopharyngeal swabs. This type of sampling is uncomfortable for the patient, dangerous for health workers, and its high demand has led to a global shortage of swabs. One of the alternative specimens is saliva. However, the optimal conditions for the test have not been established. Methods: Reverse transcription-polymerase chain reaction was used to detect the viral genome in saliva samples kept at room temperature, in the fridge or frozen for 2 days. In addition, the influence of brushing teeth and feeding on the detection of the virus in saliva was addressed. Finally, the efficiency of saliva in revealing the presence of the virus during the hospitalization period was determined in children. Results: The viral genome was consistently detected regardless of the storage conditions of saliva samples. Brushing teeth and feeding did not influence the sensitivity of the test. In hospitalized children, positive results were obtained only during the early days. Conclusions: These results support the idea of the use of saliva as an alternative specimen for diagnostic testing for COVID-19. The viral genome is stable and endures perturbations in the oral cavity. However, clearance of the virus from the mouth during the infection may limit the use of the test only to the early stages of the disease.
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #869466
    Datenquelle COVID19

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