Artikel ; Online: Simplified Mass Spectrometric Analysis of Ceramides using a Common Collision Energy.
2019 Band 54, Heft 8, Seite(n) 471–477
Abstract: Ceramides (CER) are biologically active sphingolipid precursors that are mechanistically linked to several pathogenic states including cancer, insulin resistance, and neurodegeneration. CER are commonly quantified through mass spectrometry-based methods ... ...
Abstract | Ceramides (CER) are biologically active sphingolipid precursors that are mechanistically linked to several pathogenic states including cancer, insulin resistance, and neurodegeneration. CER are commonly quantified through mass spectrometry-based methods founded upon a product ion scan (PIS) in positive mode to produce a characteristic m/z 264 ion. The ionization efficiency (IE) of CER species decreases with an increase in CER mass, thus quantitation of CER typically involves application of mass-dependent response factors (RF) for each CER species. In this work, we observed that the RF were systematically dependent on the number of fatty acid acyl carbons and the collision energy (CE) used to generate the m/z 264 ion. Using these complimentary trends, we determined an "isosbestic" CE where the RF for all CER species were equivalent, allowing for CER quantitation without postcollection correction factors. A comparison of this common CE/common RF method to the multiple RF method demonstrated good agreement between the two methods. Use of the common CE/common RF method will reduce data processing and reduce the use of multiple CER species standards. |
---|---|
Mesh-Begriff(e) | Animals ; Calibration ; Ceramides/analysis ; Ceramides/blood ; Liver/chemistry ; Male ; Mice ; Mice, Inbred C57BL ; Solvents/chemistry ; Spectrometry, Mass, Electrospray Ionization |
Chemische Substanzen | Ceramides ; Solvents |
Sprache | Englisch |
Erscheinungsdatum | 2019-07-24 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. |
ZDB-ID | 241539-2 |
ISSN | 1558-9307 ; 0024-4201 |
ISSN (online) | 1558-9307 |
ISSN | 0024-4201 |
DOI | 10.1002/lipd.12179 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Volltext online
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
Zs.A 645: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.