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  1. Artikel ; Online: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.

    Sprink, Thorben / Hartung, Frank

    International journal of molecular sciences

    2021  Band 22, Heft 17

    Abstract: In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. ... ...

    Abstract In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In
    Mesh-Begriff(e) Arabidopsis/chemistry ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Arabidopsis Proteins/physiology ; DNA Breaks, Double-Stranded ; DNA-Binding Proteins/metabolism ; Endodeoxyribonucleases/chemistry ; Endodeoxyribonucleases/genetics ; Endodeoxyribonucleases/metabolism ; Endodeoxyribonucleases/physiology ; Meiosis/physiology ; RNA Splicing/genetics ; Rad51 Recombinase/metabolism ; Recombination, Genetic ; Species Specificity
    Chemische Substanzen ASY1 protein, Arabidopsis ; Arabidopsis Proteins ; DNA-Binding Proteins ; ATRAD51 protein, Arabidopsis (EC 2.7.7.-) ; Rad51 Recombinase (EC 2.7.7.-) ; Endodeoxyribonucleases (EC 3.1.-) ; meiotic recombination protein SPO11 (EC 3.1.-)
    Sprache Englisch
    Erscheinungsdatum 2021-08-28
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22179346
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Genome editing around the globe: An update on policies and perceptions.

    Sprink, Thorben / Wilhelm, Ralf / Hartung, Frank

    Plant physiology

    2022  Band 190, Heft 3, Seite(n) 1579–1587

    Abstract: A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many ... ...

    Abstract A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many countries have made decisions on the regulatory status of products of genome editing, by exempting some kinds of edits from the classical GMO regulation. However, the guidance differs between countries even in the same region. Several countries are still debating the issue or are in the progress of updating guidance and regulatory systems to cover products of genome editing. The current global situation of different regulatory systems is putting a harmonized framework on genome-edited crops in the far future. In this update, we summarize the current developments in the field of regulation concerning edited crops and present a short insight into perception of genome editing in the society.
    Mesh-Begriff(e) Gene Editing ; Genome, Plant/genetics ; CRISPR-Cas Systems/genetics ; Crops, Agricultural/genetics ; Policy
    Sprache Englisch
    Erscheinungsdatum 2022-11-02
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiac359
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Buch ; Audio / Video ; Online: CRISPR/Cas9 und andere Genome Editing Techniken

    Hartung, Frank

    2017  

    Schlagwörter ddc:630
    Sprache Deutsch
    Erscheinungsdatum 2017-03-13
    Erscheinungsland de
    Dokumenttyp Buch ; Audio / Video ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: CRISPR/Cas9 und andere Genome Editing Techniken

    Hartung, Frank

    2017  

    Schlagwörter Text ; abstract_or_summary ; ddc:630
    Sprache Deutsch
    Erscheinungsdatum 2017-03-13
    Verlag Julius Kühn-Institut
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern

    Sprink, Thorben / Hartung, Frank

    2021  

    Abstract: In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In Arabidopsis and ... ...

    Abstract In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In Arabidopsis and other plants, both meiotic SPO11 are indispensable in a functional form for double strand break induction during meiotic prophase I. This stands in contrast to mammals and fungi, where a single SPO11 is present and sufficient. We aimed to investigate the specific function and evolution of both meiotic SPO11 paralogs in land plants. By performing immunostaining of both SPO11-1 and -2, an investigation of the spatiotemporal localization of each SPO11 during meiosis was achieved. We further exchanged SPO11-1 and -2 in Arabidopsis and could show a species-specific function of the respective SPO11. By additional changes of regions between SPO11-1 and -2, a sequence-specific function for both the SPO11 proteins was revealed. Furthermore, the previous findings about the aberrant splicing of each SPO11 were refined by narrowing them down to a specific developmental phase. These findings let us suggest that the function of both SPO11 paralogs is highly sequence specific and that the orthologs are species specific.
    Schlagwörter Text ; ddc:630 ; meiosis -- SPO11 -- double strand breaks -- DSB -- plant -- splicing -- immunostaining
    Thema/Rubrik (Code) 580
    Sprache Englisch
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Genome editing around the globe

    Sprink, Thorben / Wilhelm, Ralf / Hartung, Frank

    An update on policies and perceptions

    2022  

    Abstract: A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many ... ...

    Abstract A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many countries have made decisions on the regulatory status of products of genome editing, by exempting some kinds of edits from the classical GMO regulation. However, the guidance differs between countries even in the same region. Several countries are still debating the issue or are in the progress of updating guidance and regulatory systems to cover products of genome editing. The current global situation of different regulatory systems is putting a harmonized framework on genome-edited crops in the far future. In this update, we summarize the current developments in the field of regulation concerning edited crops and present a short insight into perception of genome editing in the society.
    Schlagwörter Text ; ddc:630
    Thema/Rubrik (Code) 320
    Sprache Englisch
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Buch ; Audio / Video ; Online: Wie CRISPR ist die Zukunft der Pflanzenzüchtung?

    Wilhelm, Ralf / Hartung, Frank / Sprink, Thorben

    2022  

    Schlagwörter Text ; ddc:630
    Sprache Deutsch
    Erscheinungsland de
    Dokumenttyp Buch ; Audio / Video ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Transgene-Free Genome Editing in Plants

    Sprink, Thorben / Hartung, Frank / Metje-Sprink, Janina

    2022  

    Abstract: Genome editing has revolutionized genetics and breeding likewise. Especially in plant breeding, it opened new ways to address traits with never known specificity. In many cases genome editing tools are provided by classical transgenic methods, i.e., by ... ...

    Abstract Genome editing has revolutionized genetics and breeding likewise. Especially in plant breeding, it opened new ways to address traits with never known specificity. In many cases genome editing tools are provided by classical transgenic methods, i.e., by Agrobacterium-based delivery, but it is also possible to perform genome editing without the use of a transgene by providing proteins, or nucleic acid protein complexes. These methods have the big advantage that transgene organisms can be avoided at any time; even transgenic intermediates are not needed. However, transgene-free methods are technically challenging, and editing rates are often lower compared to classical methods. Nevertheless, it offers great opportunities to produce plants without the need of any transgene, simplifying the regulatory processes in many jurisdictions around the globe. In this chapter, we present methods and delivery methods that can be used for transgene-free editing and present first promising examples.
    Schlagwörter Text ; ddc:630 ; Genome editing -- Mito TALEs -- Meganucleases -- Plastid editing -- Transgene-free methods
    Sprache Englisch
    Verlag Springer International Publishing
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel: Genome-edited plants in the field

    Metje-Sprink, Janina / Sprink, Thorben / Hartung, Frank

    Current opinion in biotechnology. 2020 Feb., v. 61

    2020  

    Abstract: The application of site directed nucleases (SDN) for Genome Editing (GE) in plant breeding and research increases exponentially in the last few years. The main research so far was on ‘proof of concept’ studies or improvement of the precision and delivery ...

    Abstract The application of site directed nucleases (SDN) for Genome Editing (GE) in plant breeding and research increases exponentially in the last few years. The main research so far was on ‘proof of concept’ studies or improvement of the precision and delivery of the SDN. Nevertheless, a reasonable amount of research is present on market-oriented applications for cash crops such as rice but also for commercially lesser interesting crops and vegetables. Reported field trials involving GE plants are scarce around the world and almost not existing in Europe. This is due to the regulatory landscape for GE plants, which is quite distinct and especially in the European Union very demanding. By far the most field trials involve GE rice varieties in the Asian area, followed up by tomato and other vegetables and crops.
    Schlagwörter European Union ; cash crops ; field experimentation ; gene editing ; genetically modified plants ; nucleases ; plant breeding ; rice ; tomatoes ; Europe
    Sprache Englisch
    Erscheinungsverlauf 2020-02
    Umfang p. 1-6.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2019.08.007
    Datenquelle NAL Katalog (AGRICOLA)

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  10. Buch ; Online ; Dissertation / Habilitation: Approaches to generate herbicide resistant Taraxacum koksaghyz by directed and undirected mutagenesis of the acetohydroxyacid synthase

    Kölzsch, Regina [Verfasser] / Gadau, Jürgen [Sonstige] / Prüfer, Dirk [Gutachter] / Hartung, Frank [Gutachter]

    2022  

    Verfasserangabe Regina Kölzsch ; Gutachter: Dirk Prüfer, Frank Hartung ; Sonstige beteiligte Person: Jürgen Gadau ; Julius Kühn-Institute, Germany; Mathematisch-Naturwissenschaftlichen Fakultät der Westfälischen Wilhelms-Universität Münster, Germany
    Schlagwörter Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
    Thema/Rubrik (Code) sg630
    Sprache Englisch
    Verlag Julius Kühn-Institut
    Erscheinungsort Quedlinburg
    Dokumenttyp Buch ; Online ; Dissertation / Habilitation
    ISBN 978-3-95547-121-7 ; 3-95547-121-7
    Datenquelle Digitale Dissertationen im Internet

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