Artikel ; Online: Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli.
2021 Band 36, Heft 5, Seite(n) 380–387
Abstract: The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on ... ...
Abstract | The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test, respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, which did not resemble that for UV implying that CAP-produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences. |
---|---|
Mesh-Begriff(e) | DNA Damage/drug effects ; DNA Fragmentation ; Dose-Response Relationship, Drug ; Drug Resistance, Bacterial ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Mutagenesis/drug effects ; Mutagens/pharmacology ; Mutation/drug effects ; Plasma Gases/pharmacology ; Ultraviolet Rays ; X-Rays |
Chemische Substanzen | Mutagens ; Plasma Gases |
Sprache | Englisch |
Erscheinungsdatum | 2021-08-29 |
Erscheinungsland | England |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 632903-2 |
ISSN | 1464-3804 ; 0267-8357 |
ISSN (online) | 1464-3804 |
ISSN | 0267-8357 |
DOI | 10.1093/mutage/geab030 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
Zs.A 2189: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.