Artikel ; Online: Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source.
2023 Band 10, Heft Pt 6, Seite(n) 729–737
Abstract: Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary ... ...
Abstract | Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourth-generation synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing. |
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Sprache | Englisch |
Erscheinungsdatum | 2023-11-01 |
Erscheinungsland | England |
Dokumenttyp | Journal Article |
ZDB-ID | 2754953-7 |
ISSN | 2052-2525 ; 2052-2525 |
ISSN (online) | 2052-2525 |
ISSN | 2052-2525 |
DOI | 10.1107/S2052252523008618 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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