Article ; Online: Tyrosinase enzyme purification and immobilization from Pseudomonas sp. EG22 using cellulose coated magnetic nanoparticles: characterization and application in melanin production.
World journal of microbiology & biotechnology
2023 Volume 40, Issue 1, Page(s) 10
Abstract: Melanin is a brown-black pigment with significant roles in various biological processes. The tyrosinase enzyme catalyzes the conversion of tyrosine to melanin and has promising uses in the pharmaceutical and biotechnology sectors. This research aims to ... ...
| Abstract | Melanin is a brown-black pigment with significant roles in various biological processes. The tyrosinase enzyme catalyzes the conversion of tyrosine to melanin and has promising uses in the pharmaceutical and biotechnology sectors. This research aims to purify and immobilize the tyrosinase enzyme from Pseudomonas sp. EG22 using cellulose-coated magnetic nanoparticles. Various techniques were utilized to examine the synthesized nanoparticles, which exhibited a spherical shape with an average diameter of 12 nm and a negative surface potential of - 55.7 mV with a polydispersity index (PDI) of 0.260. Comparing the immobilized magnetic tyrosinase enzyme with the free enzyme, the study's findings showed that the immobilized tyrosinase enzyme had optimal activity at a pH of 6 and a temperature of 35 °C, and its activity increased as the concentration of tyrosine increased. The study investigated the antibacterial and anticancer bioactivity of the enzyme's melanin product and found that it exhibited potential antibacterial activity against a multi-drug resistant strain including S. aureus and E. coli. The produced melanin also demonstrated the potential to decrease cell survival and induce apoptosis in initiation cells. |
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| MeSH term(s) | Monophenol Monooxygenase ; Melanins ; Cellulose ; Magnetite Nanoparticles/chemistry ; Pseudomonas ; Escherichia coli ; Staphylococcus aureus ; Enzymes, Immobilized/chemistry ; Tyrosine ; Anti-Bacterial Agents/pharmacology |
| Chemical Substances | Monophenol Monooxygenase (EC 1.14.18.1) ; Melanins ; Cellulose (9004-34-6) ; Magnetite Nanoparticles ; Enzymes, Immobilized ; Tyrosine (42HK56048U) ; Anti-Bacterial Agents |
| Language | English |
| Publishing date | 2023-11-10 |
| Publishing country | Germany |
| Document type | Journal Article |
| ZDB-ID | 1499109-3 |
| ISSN | 1573-0972 ; 0959-3993 |
| ISSN (online) | 1573-0972 |
| ISSN | 0959-3993 |
| DOI | 10.1007/s11274-023-03796-w |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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