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  1. Artikel ; Online: Structural atlas of human primary microRNAs generated by SHAPE-MaP.

    Baek, S Chan / Kim, Boseon / Jang, Harim / Kim, Kijun / Park, Il-Soo / Min, Dal-Hee / Kim, V Narry

    Molecular cell

    2024  Band 84, Heft 6, Seite(n) 1158–1172.e6

    Abstract: MicroRNA (miRNA) maturation is critically dependent on structural features of primary transcripts (pri-miRNAs). However, the scarcity of determined pri-miRNA structures has limited our understanding of miRNA maturation. Here, we employed selective 2'- ... ...

    Abstract MicroRNA (miRNA) maturation is critically dependent on structural features of primary transcripts (pri-miRNAs). However, the scarcity of determined pri-miRNA structures has limited our understanding of miRNA maturation. Here, we employed selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP), a high-throughput RNA structure probing method, to unravel the secondary structures of 476 high-confidence human pri-miRNAs. Our SHAPE-based structures diverge substantially from those inferred solely from computation, particularly in the apical loop and basal segments, underlining the need for experimental data in RNA structure prediction. By comparing the structures with high-throughput processing data, we determined the optimal structural features of pri-miRNAs. The sequence determinants are influenced substantially by their structural contexts. Moreover, we identified an element termed the bulged GWG motif (bGWG) with a 3' bulge in the lower stem, which promotes processing. Our structure-function mapping better annotates the determinants of pri-miRNA processing and offers practical implications for designing small hairpin RNAs and predicting the impacts of miRNA mutations.
    Mesh-Begriff(e) Humans ; RNA Processing, Post-Transcriptional ; MicroRNAs/metabolism ; RNA, Small Interfering ; Ribonuclease III/genetics
    Chemische Substanzen MicroRNAs ; RNA, Small Interfering ; Ribonuclease III (EC 3.1.26.3)
    Sprache Englisch
    Erscheinungsdatum 2024-03-05
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2024.02.005
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Hybridization-Controlled Pseudogap State in the Quantum Critical Superconductor CeCoIn_{5}.

    Jang, Harim / Thi Anh Hong, Vuong / Kim, Jihyun / Lu, Xin / Park, Tuson

    Physical review letters

    2023  Band 130, Heft 7, Seite(n) 76301

    Abstract: The origin of the partial suppression of the electronic density states in the enigmatic pseudogap behavior, which is at the core of understanding high-T_{c} superconductivity, has been hotly contested as either a hallmark of preformed Cooper pairs or an ... ...

    Abstract The origin of the partial suppression of the electronic density states in the enigmatic pseudogap behavior, which is at the core of understanding high-T_{c} superconductivity, has been hotly contested as either a hallmark of preformed Cooper pairs or an incipient order of competing interactions nearby. Here, we report the quasiparticle scattering spectroscopy of the quantum critical superconductor CeCoIn_{5}, where a pseudogap with energy Δ_{g} was manifested as a dip in the differential conductance (dI/dV) below the characteristic temperature of T_{g}. When subjected to external pressure, T_{g} and Δ_{g} gradually increase, following the trend of increase in quantum entangled hybridization between the Ce 4f moment and conduction electrons. On the other hand, the superconducting (SC) energy gap and its phase transition temperature shows a maximum, revealing a dome shape under pressure. The disparate dependence on pressure between the two quantum states shows that the pseudogap is less likely involved in the formation of SC Cooper pairs, but rather is controlled by Kondo hybridization, indicating that a novel type of pseudogap is realized in CeCoIn_{5}.
    Sprache Englisch
    Erscheinungsdatum 2023-03-02
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.130.076301
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Evidence for charge delocalization crossover in the quantum critical superconductor CeRhIn

    Wang, Honghong / Park, Tae Beom / Kim, Jihyun / Jang, Harim / Bauer, Eric D / Thompson, Joe D / Park, Tuson

    Nature communications

    2023  Band 14, Heft 1, Seite(n) 7341

    Abstract: The nature of charge degrees-of-freedom distinguishes scenarios for interpreting the character of a second order magnetic transition at zero temperature, that is, a magnetic quantum critical point (QCP). Heavy-fermion systems are prototypes of this ... ...

    Abstract The nature of charge degrees-of-freedom distinguishes scenarios for interpreting the character of a second order magnetic transition at zero temperature, that is, a magnetic quantum critical point (QCP). Heavy-fermion systems are prototypes of this paradigm, and in those, the relevant question is where, relative to a magnetic QCP, does the Kondo effect delocalize their f-electron degrees-of-freedom. Herein, we use pressure-dependent Hall measurements to identify a finite-temperature scale E
    Sprache Englisch
    Erscheinungsdatum 2023-11-13
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42965-1
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: A Quenched Disorder in the Quantum-Critical Superconductor CeCoIn

    Jung, Soon-Gil / Jang, Harim / Kim, Jihyun / Park, Jin-Hong / Lee, Sangyun / Seo, Soonbeom / Bauer, Eric D / Park, Tuson

    Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    2023  Band 11, Heft 1, Seite(n) e2304837

    Abstract: Emergent inhomogeneous electronic phases in metallic quantum systems are crucial for understanding high- ... ...

    Abstract Emergent inhomogeneous electronic phases in metallic quantum systems are crucial for understanding high-T
    Sprache Englisch
    Erscheinungsdatum 2023-11-20
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 2808093-2
    ISSN 2198-3844 ; 2198-3844
    ISSN (online) 2198-3844
    ISSN 2198-3844
    DOI 10.1002/advs.202304837
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8.

    Kwon, S Chul / Jang, Harim / Shen, Siyuan / Baek, S Chan / Kim, Kijun / Yang, Jihye / Kim, Jeesoo / Kim, Jong-Seo / Wang, Suman / Shi, Yunyu / Li, Fudong / Kim, V Narry

    Nucleic acids research

    2020  Band 48, Heft 19, Seite(n) 11097–11112

    Abstract: The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a ... ...

    Abstract The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate 'cluster assistance' in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway.
    Mesh-Begriff(e) Cell Cycle Proteins/metabolism ; HCT116 Cells ; HEK293 Cells ; Humans ; K562 Cells ; MicroRNAs/metabolism ; Protein Binding ; Protein Conformation ; RNA Processing, Post-Transcriptional ; RNA-Binding Proteins/metabolism ; Transcription Factors/metabolism
    Chemische Substanzen Cell Cycle Proteins ; DGCR8 protein, human ; ERH protein, human ; MIRN144 microRNA, human ; MIRN451 microRNA, human ; MicroRNAs ; RNA-Binding Proteins ; Transcription Factors
    Sprache Englisch
    Erscheinungsdatum 2020-11-11
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkaa827
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

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