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  1. Artikel ; Online: Cohort study of the effects of occupation and environmental tobacco smoke on the incidence of Alzheimer's disease among seniors.

    Yang, Li / Wan, Wenjie / Xuan, Cheng / Yu, Caiyan / Jin, Kailing / Zheng, Pinpin / Yan, Jing

    Tobacco induced diseases

    2023  Band 21, Seite(n) 18

    Abstract: Introduction: Alzheimer's disease (AD) is a disease caused by many factors including occupational and environmental factors. Secondhand smoke (SHS) can affect cognitive function. However, there is limited recent epidemiological research on how SHS and ... ...

    Abstract Introduction: Alzheimer's disease (AD) is a disease caused by many factors including occupational and environmental factors. Secondhand smoke (SHS) can affect cognitive function. However, there is limited recent epidemiological research on how SHS and occupational factors affect AD in Zhejiang province.
    Methods: We established a cohort of an AD high-risk population. In 2018, a cohort of 1742 elderly aged ≥60 years was established. In 2020, the cohort was followed up, and a total of 1545 people participated in the two surveys. Data collection included demographic and economic information such as age, gender, occupation, education level etc., and relative health behavior information such as smoking, drinking and tea drinking, etc. Basic physical examination data included height, weight, blood pressure, etc. At the same time, related cognitive status was assessed. Cox proportional hazards regression models, both unadjusted and adjusted models, were used to determine associations between cohort characteristics and AD.
    Results: The results showed that SHS exposure and occupational characteristics were associated with an increased risk of cognitive impairments in seniors. Subgroups who used to work as blue-collar workers, who never worked, who kept standing for most of the time at work, and who were engaged in hard physical labor prior to retirement, had high incidence rates of AD.
    Conclusions: It was revealed that SHS, standing for most of the time and hard physical labor were associated risk factors of AD among seniors, compared with white-collar work. We recommend that the government establish a community supervisory mechanism to persuade smokers to quit or control smoking.
    Sprache Englisch
    Erscheinungsdatum 2023-02-06
    Erscheinungsland Greece
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2194616-4
    ISSN 1617-9625 ; 1617-9625
    ISSN (online) 1617-9625
    ISSN 1617-9625
    DOI 10.18332/tid/157208
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Rapid and visual monitoring of alien sequences using crop wild relatives specific oligo-painting: The case of cucumber chromosome engineering

    Zhao, Qinzheng / Jin, Kailing / Hu, Wei / Qian, Chuntao / Li, Ji / Zhang, Wenli / Lou, Qunfeng / Chen, Jinfeng

    Plant science. 2022 June, v. 319

    2022  

    Abstract: Wild species related to domesticated crops (crop wild relatives, or CWRs) represent a high level of genetic diversity that provides a practical gene pool for crop pre-breeding employed to address climate change and food demand challenges globally. ... ...

    Abstract Wild species related to domesticated crops (crop wild relatives, or CWRs) represent a high level of genetic diversity that provides a practical gene pool for crop pre-breeding employed to address climate change and food demand challenges globally. Nevertheless, rapid identifying and visual tracking of alien chromosomes and sequences derived from CWRs have been a technical challenge for crop chromosome engineering. Here, a species-specific oligonucleotide (oligo) pool was developed by using the reference genome of Cucumis hystrix (HH, 2n = 2x = 24), a wild species carrying many favorable traits and interspecific compatibility with cultivated cucumber (C. sativus, CC, 2n = 2x = 14). These synthetic double-stranded oligo probes were applied to validate the assembly and characterize the chromosome architectures of C. hystrix, as well as to rapidly identify C. hystrix-chromosomes in diverse C. sativus-hystrix chromosome-engineered germplasms, including interspecific hybrid F1 (HC), synthetic allopolyploids (HHCC, CHC, and HCH) and alien additional lines (CC-H). Moreover, a ∼2Mb of C. hystrix-specific sequences, introduced into cultivated cucumber, were visualized by CWR-specific oligo-painting. These results demonstrate that the CWR-specific oligo-painting technique holds broad applicability for chromosome engineering of numerous crops, as it allows rapid identification of alien chromosomes, reliable detection of homoeologous recombination, and visual tracking of the introgression process. It is promising to achieve directed and high-precision crop pre-breeding combined with other breeding techniques, such as CRISPR/Cas9-mediated chromosome engineering.
    Schlagwörter CRISPR-Cas systems ; Cucumis hystrix ; allopolyploidy ; chromosomes ; climate change ; cucumbers ; gene pool ; genetic variation ; genome ; germplasm ; hybrids ; introgression ; oligonucleotides
    Sprache Englisch
    Erscheinungsverlauf 2022-06
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 742010-9
    ISSN 1873-2259 ; 0168-9452
    ISSN (online) 1873-2259
    ISSN 0168-9452
    DOI 10.1016/j.plantsci.2022.111199
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel ; Online: Rapid and visual monitoring of alien sequences using crop wild relatives specific oligo-painting: The case of cucumber chromosome engineering.

    Zhao, Qinzheng / Jin, Kailing / Hu, Wei / Qian, Chuntao / Li, Ji / Zhang, Wenli / Lou, Qunfeng / Chen, Jinfeng

    Plant science : an international journal of experimental plant biology

    2022  Band 319, Seite(n) 111199

    Abstract: Wild species related to domesticated crops (crop wild relatives, or CWRs) represent a high level of genetic diversity that provides a practical gene pool for crop pre-breeding employed to address climate change and food demand challenges globally. ... ...

    Abstract Wild species related to domesticated crops (crop wild relatives, or CWRs) represent a high level of genetic diversity that provides a practical gene pool for crop pre-breeding employed to address climate change and food demand challenges globally. Nevertheless, rapid identifying and visual tracking of alien chromosomes and sequences derived from CWRs have been a technical challenge for crop chromosome engineering. Here, a species-specific oligonucleotide (oligo) pool was developed by using the reference genome of Cucumis hystrix (HH, 2n = 2x = 24), a wild species carrying many favorable traits and interspecific compatibility with cultivated cucumber (C. sativus, CC, 2n = 2x = 14). These synthetic double-stranded oligo probes were applied to validate the assembly and characterize the chromosome architectures of C. hystrix, as well as to rapidly identify C. hystrix-chromosomes in diverse C. sativus-hystrix chromosome-engineered germplasms, including interspecific hybrid F1 (HC), synthetic allopolyploids (HHCC, CHC, and HCH) and alien additional lines (CC-H). Moreover, a ∼2Mb of C. hystrix-specific sequences, introduced into cultivated cucumber, were visualized by CWR-specific oligo-painting. These results demonstrate that the CWR-specific oligo-painting technique holds broad applicability for chromosome engineering of numerous crops, as it allows rapid identification of alien chromosomes, reliable detection of homoeologous recombination, and visual tracking of the introgression process. It is promising to achieve directed and high-precision crop pre-breeding combined with other breeding techniques, such as CRISPR/Cas9-mediated chromosome engineering.
    Mesh-Begriff(e) Chromosomes, Plant/genetics ; Crops, Agricultural/genetics ; Cucumis sativus/genetics ; Gene Pool ; Introduced Species ; Plant Breeding/methods
    Sprache Englisch
    Erscheinungsdatum 2022-02-22
    Erscheinungsland Ireland
    Dokumenttyp Journal Article
    ZDB-ID 742010-9
    ISSN 1873-2259 ; 0168-9452
    ISSN (online) 1873-2259
    ISSN 0168-9452
    DOI 10.1016/j.plantsci.2022.111199
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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