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  1. Artikel ; Online: Knockdown of long non-coding RNA SNHG3 inhibits proliferation, migration and invasion of human thyroid cancer via miR-339-5p/GPR62 axis

    Jin Tang / Xiao-xia Huang

    Heliyon, Vol 9, Iss 9, Pp e19713- (2023)

    2023  

    Abstract: Previous studies have implicated SNHG3, a long non-coding RNA, in various human cancers, suggesting its oncogenic role. However, its specific involvement in thyroid cancer and the underlying molecular mechanisms remain unclear. Therefore, this study aims ...

    Abstract Previous studies have implicated SNHG3, a long non-coding RNA, in various human cancers, suggesting its oncogenic role. However, its specific involvement in thyroid cancer and the underlying molecular mechanisms remain unclear. Therefore, this study aims to elucidate the role of SNHG3 in human thyroid cancer and its interaction with the miR-339-5p/GPR62 axis. Understanding these mechanisms could provide insights into potential therapeutic targets for managing thyroid cancer. Results revealed significant upregulation of SNHG3 in human thyroid cancer tissues and cell lines. Knockdown of SNHG3 significantly suppressed proliferation, migration and invasion of CUTC5 and IHH-4 thyroid cancer cells. Knockdown of SNHG3 induces apoptosis in CUTC5 and IHH-4 cells and also inhibits the growth of xenografted tumors in vivo. Different in vitro assays revealed the interaction of SNHG3 with microRNA-339-5p (miR-339-5p) in thyroid cancer cells. Expression of miR-339-5p was significantly downregulated in thyroid cancer tissues and cell lines. However, the knockdown of SNHG3 caused significant upregulation of miR-339-5p. Interestingly, overexpression of miR-339-5p exerted tumor-suppressive effects in CUTC5 and IHH-4 cells via post-transcriptional suppression of GPR62. Knockdown of GPR62 significantly inhibited the proliferation, migration and invasion of CUTC5 and IHH-4 cells. Nonetheless, inhibition of miR-339-5p or overexpression of GPR62 avoids the growth inhibitory effects of SNHG3 knockdown in CUTC5 and IHH-4 cells. Results indicated that SNHG3 exerts oncogenic molecular function in thyroid cancer via miR-339-5p/GPR62 axis and may act as a therapeutic target for its management.
    Schlagwörter Thyroid cancer ; Long non-coding RNA ; SNHG3 ; Apoptosis ; miR-339-p ; GPR62 ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2023-09-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Perfluorooctane sulfonate (PFOS) causes aging damage in the liver through the mt-DNA-mediated NLRP3 signaling pathway.

    Xiaopeng, Cui / Jin, Tang

    Ecotoxicology and environmental safety

    2023  Band 262, Seite(n) 115121

    Abstract: Perfluorooctane sulfonate (PFOS) is not readily degradable in the natural environment, and PFOS is widely used in industry. Globally, PFOS exposure occurs in the environment. PFOS is persistent and non-biodegradable. The general public can come into ... ...

    Abstract Perfluorooctane sulfonate (PFOS) is not readily degradable in the natural environment, and PFOS is widely used in industry. Globally, PFOS exposure occurs in the environment. PFOS is persistent and non-biodegradable. The general public can come into contact with PFOS by inhaling PFOS-contaminated dust and air, drinking contaminated water, eating contaminated food. Thus, PFOS induces potential health damage globally. In this study, the effect of PFOS on aging of the liver was investigated. In an in vitro cellular model, a series of biochemical experiments were conducted via cell proliferation assays, flow cytometry, immunocytochemistry and laser confocal microscopy. It was found that PFOS led to hepatocyte senescence via Sa-β-gal staining and detection of senescence markers (p16, p21 and p53). In addition, PFOS also led to oxidative stress and inflammation. Mechanistic studies have shown that PFOS can lead to elevated mitochondrial ROS in hepatocytes through calcium overload. ROS cause alterations in mitochondrial membrane potential, subsequently inducing mPTP (mitochondrial permeability transition pore) opening, which in turn releases mt-DNA from mitochondria into the cytoplasm, thus activating NLRP3, which causes the senescence of hepatocytes. Based on this, we further analyzed the effect of PFOS on liver aging in vivo and found that PFOS caused the aging of liver tissues. On this basis, we preliminarily investigated the effect of β-carotene on the aging damage caused by PFOS and found that it could alleviate the liver aging caused by PFOS. In summary, the current study shows that PFOS causes aging damage to the liver, and this study provides a more in-depth understanding of the toxicity characteristics of PFOS.
    Sprache Englisch
    Erscheinungsdatum 2023-06-27
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 436536-7
    ISSN 1090-2414 ; 0147-6513
    ISSN (online) 1090-2414
    ISSN 0147-6513
    DOI 10.1016/j.ecoenv.2023.115121
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Preparation of DNA interstrand cross-link repair intermediates induced by abasic sites

    Jin Tang / Linlin Zhao

    MethodsX, Vol 9, Iss , Pp 101687- (2022)

    2022  

    Abstract: DNA interstrand cross-links (ICLs) are extremely deleterious DNA lesions, which can block different DNA transactions. A major step in ICL repair involves strand cleavage activities flanking the cross-linking site, also known as unhooking. The cleavage ... ...

    Abstract DNA interstrand cross-links (ICLs) are extremely deleterious DNA lesions, which can block different DNA transactions. A major step in ICL repair involves strand cleavage activities flanking the cross-linking site, also known as unhooking. The cleavage generates a single-stranded DNA remnant attached to the unbroken strand, often referred to as the unhooked ICL repair intermediates. The unhooked ICLs are substrates for specialized DNA polymerases, leading to the eventual restoration of the duplex DNA structure. Although these repair events have been outlined, the understanding of molecular details of the repair pathways has been hindered by the difficulty of preparing structurally defined ICL repair intermediates. Here, we present a straightforward method to prepare model ICL repair intermediates derived from a ubiquitous type of endogenous DNA modification, abasic (AP) sites. AP-derived ICLs have emerged as an important type of endogenous ICLs. We developed the method based on commercially available materials without the requirement of synthetic chemistry expertise. The method is expected to be accessible to any interested labs in the DNA repair community.• The method exploits the alkaline lability of ribonucleotides and uses designer oligonucleotides to create ICL repair intermediates with varying lengths of the unhooked strand.• Strand cleavage at ribonucleotides is achieved using NaOH, which avoids the potential for incomplete digestion during enzymatic workup due to specific substrate structures.• The method is grounded on the high cross-linking yield between an AP lesion and a nucleotide analog, 2-aminopurine, via reductive amination, developed by Gates and colleagues.
    Schlagwörter Preparation of DNA interstrand cross-link repair intermediates induced by abasic sites ; Science ; Q
    Thema/Rubrik (Code) 500
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Ziziphora clinopodioides Lam leaf aqueous extract mediated novel green synthesis of iron nanoparticles and its anti-hemolytic anemia potential

    Shengmei Chen / Ailing Fang / Yanfa Zhong / Jin Tang

    Arabian Journal of Chemistry, Vol 15, Iss 3, Pp 103561- (2022)

    A chemobiological study

    2022  

    Abstract: In this study, the aqueous extract Ziziphora clinopodioides was used to biosynthesis of iron nanoparticles. A green, productive, and environmentally method was developed for the valuable study and the effective preparation of the green-synthesis of iron ... ...

    Abstract In this study, the aqueous extract Ziziphora clinopodioides was used to biosynthesis of iron nanoparticles. A green, productive, and environmentally method was developed for the valuable study and the effective preparation of the green-synthesis of iron nanoparticles using aqueous extracts from the leaf of Ziziphora clinopodioides as a result of reducing and stabilizing factor. The simplicity of the synthesis procedures and easy work up are the benefits of the present study. The structural and morphological characterization of green‐synthesized FeNPs was performed by Uv–Vis. and FT-IR spectroscopy, XRD, SEM, and EDX techniques. The SEM images have exhibited an equal and uniform spherical morphology in size of 30.04. We also investigated the anti-hemolytic anemia property of FeNPs in an animal model of hemolytic anemia. In vivo assay, induction of hemolytic anemia was done by phenylhydrazine in mice. FeNPs significantly reduced the weight and volume of liver and spleen and the concentration of pro-inflammatory cytokines and increased the body weight, the anti-inflammatory cytokines concentration, and the total platelet, WBC, neutrophil, lymphocyte, eosinophil, monocyte, and basophil counts, and RBC parameters as compared to the untreated mice. About the biochemical parameters, FeNPs significantly increased GPx, CAT, and SOD in serum, liver, and spleen, and also HDL, total protein, and albumin in serum, and decreased GR in serum, liver, and spleen, and also erythropoietin, ferritin, ferrous, creatinine, urea, LDL, triglyceride, cholesterol, GGT, ALT, AST, and ALP in serum as compared to the anemic mice. DPPH test revealed similar antioxidant potentials for FeNPs and Butylated hydroxytoluene. FeNPs had low cell viability dose-dependently against HUVEC cell line. It appears that FeNPs can be administrated as a hematoprotective and anti-hemolytic anemia drug or supplement for the treatment of hemolytic anemia in the clinical trial.
    Schlagwörter Ziziphora clinopodioides Lam leaf ; Iron nanoparticles ; Hemolytic anemia ; Mouse model of hemolytic anemia ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2022-03-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Cross-Modality Person Re-Identification Based on Heterogeneous Center Loss and Non-Local Features

    Chengmei Han / Peng Pan / Aihua Zheng / Jin Tang

    Entropy, Vol 23, Iss 919, p

    2021  Band 919

    Abstract: Cross-modality person re-identification is the study of images of people matching under different modalities (RGB modality, IR modality). Given one RGB image of a pedestrian collected under visible light in the daytime, cross-modality person re- ... ...

    Abstract Cross-modality person re-identification is the study of images of people matching under different modalities (RGB modality, IR modality). Given one RGB image of a pedestrian collected under visible light in the daytime, cross-modality person re-identification aims to determine whether the same pedestrian appears in infrared images (IR images) collected by infrared cameras at night, and vice versa. Cross-modality person re-identification can solve the task of pedestrian recognition in low light or at night. This paper aims to improve the degree of similarity for the same pedestrian in two modalities by improving the feature expression ability of the network and designing appropriate loss functions. To implement our approach, we introduce a deep neural network structure combining heterogeneous center loss (HC loss) and a non-local mechanism. On the one hand, this can heighten the performance of feature representation of the feature learning module, and, on the other hand, it can improve the similarity of cross-modality within the class. Experimental data show that the network achieves excellent performance on SYSU-MM01 datasets.
    Schlagwörter cross-modality ; person re-identification ; heterogeneous center loss ; non-local ; Science ; Q ; Astrophysics ; QB460-466 ; Physics ; QC1-999
    Thema/Rubrik (Code) 380
    Sprache Englisch
    Erscheinungsdatum 2021-07-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Anterior atlantoaxial motion preservation fixation technique for axis complex fractures (odontoid process with Hangman’s fractures) and technique notes

    Qilin Lu / Jin Tang / Wei Xie / Xianzhong Mei / Hui Kang / Ximing Liu / Feng Xu / Xianhua Cai

    Scientific Reports, Vol 14, Iss 1, Pp 1-

    2024  Band 8

    Abstract: Abstract This study aims to investigate the feasibility and efficacy of anterior atlantoaxial motion preservation fixation (AMPF) in treating axis complex fractures involving the odontoid process fracture and Hangman’s fractures with C2/3 instability. A ... ...

    Abstract Abstract This study aims to investigate the feasibility and efficacy of anterior atlantoaxial motion preservation fixation (AMPF) in treating axis complex fractures involving the odontoid process fracture and Hangman’s fractures with C2/3 instability. A retrospective study was conducted on eight patients who underwent AMPF for axis complex fractures at the General Hospital of Central Theater Command from February 2004 to October 2021. The types of axis injuries, reasons for injuries, surgery time, intraoperative blood loss, spinal cord injury classification (American Spinal Injury Association, ASIA), as well as complications and technical notes, were documented. This study included eight cases of type II Hangman’s fracture, five cases of type II and three cases of type III odontoid process fracture. Five patients experienced traffic accidents, while three patients experienced falling injuries. All patients underwent AMPF surgery with an average intraoperative blood loss of 288.75 mL and a duration of 174.5 min. Two patients experienced dysphagia 1 month after surgery. The patients were followed up for an average of 15.63 months. One case improved from C to E in terms of neurological condition, three cases improved from D to E, and four cases remained at E. Bony fusion and Atlantoaxial Motion Preservation were successfully achieved for all eight patients. AMPF is a feasible and effective way for simultaneous odontoid process fracture and Hangman’s fractures with C2/3 instability, while preserving atlantoaxial movement.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2024-01-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Essential role of the amino-terminal region of Drosha for the Microprocessor function

    Amit Prabhakar / Song Hu / Jin Tang / Prajakta Ghatpande / Giorgio Lagna / Xuan Jiang / Akiko Hata

    iScience, Vol 26, Iss 10, Pp 107971- (2023)

    2023  

    Abstract: Summary: Drosha is a core component of the Microprocessor complex that cleaves primary-microRNAs (pri-miRNAs) to generate precursor-miRNA and regulates the expression of ∼80 ribosomal protein (RP) genes. Despite the fact that mutations in the amino- ... ...

    Abstract Summary: Drosha is a core component of the Microprocessor complex that cleaves primary-microRNAs (pri-miRNAs) to generate precursor-miRNA and regulates the expression of ∼80 ribosomal protein (RP) genes. Despite the fact that mutations in the amino-terminal region of Drosha (Drosha-NTR) are associated with a vascular disorder, hereditary hemorrhagic telangiectasia, the precise function of Drosha-NTR remains unclear. By deleting exon 5 from the Drosha gene and generating a Drosha mutant lacking the NTR (ΔN), we demonstrate that ΔN is unable to process pri-miRNAs, which leads to a global miRNA depletion, except for the miR-183/96/182 cluster. We find that Argonaute 2 facilitates the processing of the pri-miR-183/96/182 in ΔN cells. Unlike full-length Drosha, ΔN is not degraded under serum starvation, resulting in unregulated RP biogenesis and protein synthesis in ΔN cells, allowing them to evade growth arrest. This study reveals the essential role of Drosha-NTR in miRNA production and nutrient-dependent translational control.
    Schlagwörter Molecular biology ; Omics ; Science ; Q
    Thema/Rubrik (Code) 612
    Sprache Englisch
    Erscheinungsdatum 2023-10-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: How Could Cooperatives Successfully Develop Their Social Responsibility

    Jin Tang / Timo Sipiläinen / Gang Fu

    Sustainability, Vol 12, Iss 9282, p

    The Perspective of Life Cycle

    2020  Band 9282

    Abstract: Social responsibility is a natural obligation of cooperatives, and fulfilling social responsibility is of great meaning to the sustainable development of cooperatives and society. This article constructs a “life cycle-cooperative social responsibility ... ...

    Abstract Social responsibility is a natural obligation of cooperatives, and fulfilling social responsibility is of great meaning to the sustainable development of cooperatives and society. This article constructs a “life cycle-cooperative social responsibility framework (LC-CoopSRF)” and analyzes the framework with the case of Chongxin Apiculture Specialized Cooperative of Sichuan Province, China. The research results show that cooperatives should respect the law of life cycle, consider conditions such as operational capabilities and ethical expectations, and fulfill social responsibility in a reasonable manner. The successful cooperative highlights the bottom-line responsibility in the establishment phase, internal responsibility in the growth phase, system responsibility in the maturity phase, and the differentiation phase is the phase of system responsibility.
    Schlagwörter cooperative ; social responsibility ; life cycle ; operational capability ; ethical expectations ; case study ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
    Thema/Rubrik (Code) 170
    Sprache Englisch
    Erscheinungsdatum 2020-11-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel ; Online: Net Carbon Sequestration Performance of Cropland Use in China’s Principal Grain-Producing Area

    Haoyue Wu / Jin Tang / Hanjiao Huang / Wenkuan Chen / Yue Meng

    Land, Vol 10, Iss 714, p

    An Evaluation and Spatiotemporal Divergence

    2021  Band 714

    Abstract: As cropland possess dual carbon effects of emitting and sequestering, giving full attention to its net carbon sequestration is an effective option for mitigating global warming. By analyzing the carbon cycle of a cropland use system, we develop an ... ...

    Abstract As cropland possess dual carbon effects of emitting and sequestering, giving full attention to its net carbon sequestration is an effective option for mitigating global warming. By analyzing the carbon cycle of a cropland use system, we develop an inventory for measuring the net carbon sequestration, covering four carbon sources, i.e., agricultural materials, rice fields, soils, straw burning, with the crop carbon sequestration considered. Different from conventional studies that have focused on quantity, in this study, we define net carbon sequestration performance of cropland use (NCSPC) as the ratio of actual net carbon sequestration to an optimal value per unit of cropland. We estimate the net carbon sequestration of cropland use, from 2000 to 2019, for the study area consisting of the 13 principal grain-producing provinces in China. Then, global-SBM is applied to measure the provincial NCSPC; furthermore, the Theil index and convergence test are employed to portray the spatiotemporal characteristics and regional divergence. The results show the following: (1) The net carbon sequestration was 3.837 t per hectare of cropland in the principal grain-producing area, of which the sequestration and the emission were 6.343 t and 2.506 t, respectively. The share of emissions, from largest to smallest, was methane from rice paddies, agricultural materials, straw burning, and soil nitrous oxide. Specifically, cropland use in Henan exhibited the strongest net carbon sequestration, whereas in Hunan it was the lowest. (2) The average NCSPC was 0.774 in the principal grain-producing area, indicating that 22.6% of the net carbon sequestration per unit of cropland remained to be explored under the corresponding production technology and input combinations. Temporally, the NCSPC had an annual change rate of −0.30%, displaying a slowly declining trend. Spatially, the NCSPC evolved from a scattered distribution to blocky agglomeration, eventually presenting a decreasing pattern from north to south. (3) First, the total Theil ...
    Schlagwörter crop carbon sequestration ; carbon emission ; performance evaluation ; spatiotemporal characteristics ; global-SBM ; convergence test ; Agriculture ; S
    Thema/Rubrik (Code) 550
    Sprache Englisch
    Erscheinungsdatum 2021-07-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: Successful repair of a tuberculous bronchial lymphatic fistula with pericardial fat pad patch. A case report.

    He, Jin Tang / Fu, Shao Wu / Zheng, Gang / Duan, Hong

    Annali italiani di chirurgia

    2022  Band 91, Seite(n) 691–696

    Abstract: This study reported a rare case of mediastinal abscess scrofula. The patient was found to have a large left main bronchial orificium fistula (approximately 1.5x1.5cm in size) after clearing a mediastinal lymph node abscess via the right thoracotomy ... ...

    Abstract This study reported a rare case of mediastinal abscess scrofula. The patient was found to have a large left main bronchial orificium fistula (approximately 1.5x1.5cm in size) after clearing a mediastinal lymph node abscess via the right thoracotomy approach. The adjacent tissue was empty and could not be directly repaired. Therefore, the research team cut out an appropriately sized right pericardial fat pad patch during the operation for repairing and then covered it with a biological patch (Neoveil) for reinforcement. The mediastinal pleura was treated by embedding and the postoperative recovery was good. KEY WORDS: Abscess, Fistula, Pericardial fat patch, Tuberculous bronchial lymphatic, Tuberculosis bacillus, Type mediastinal lymphatic tuberculosis.
    Mesh-Begriff(e) Adipose Tissue ; Bronchial Fistula/etiology ; Bronchial Fistula/surgery ; Humans ; Pericardium ; Thoracotomy ; Tuberculosis
    Sprache Englisch
    Erscheinungsdatum 2022-02-15
    Erscheinungsland Italy
    Dokumenttyp Case Reports ; Journal Article
    ZDB-ID 418927-9
    ISSN 2239-253X ; 0003-469X
    ISSN (online) 2239-253X
    ISSN 0003-469X
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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