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  1. Article: Tobacco‐specific nitrosamines – Determination of N‐nitrosoanabasine, N‐nitrosoanatabine, N‐nitrosonornicotine and 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanol in urine by LC‐MS/MS : Biomonitoring methods, 2019

    Deutsche Forschungsgemeinschaft. Ständige Senatskommission zur Prüfung Gesundheitsschädlicher Arbeitsstoffe / Scherer, Gerhard / Gilch, Gerhard / Köhler, Dominique / Völkel, Wolfgang / Göen, Thomas / Hartwig, Andrea / MAK Commission

    The MAK collection for occupational health and safety, 4(4):2416-2441

    2019  

    Abstract: The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. This analytical method ... ...

    Institution Deutsche Forschungsgemeinschaft. Ständige Senatskommission zur Prüfung Gesundheitsschädlicher Arbeitsstoffe
    Abstract The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. This analytical method permits the determination of tobacco‐specific nitrosamines (TSNA) in urine using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). The parameters in question are N‐nitrosoanabasine (NAB), N‐nitrosoanatabine (NAT), N‐nitrosonornicotine (NNN) and 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanol (NNAL). NNAL is a metabolite of 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone (NNK). Due to its sensitivity, this method is suitable for the detection of the aforementioned analytes in the urine of smokers. NNAL can also be quantified in the urine of passive smokers. The analytes NAB, NAT, NNN and NNAL are present in urine in both free and glucuronidated forms. For the determination of the total TSNA level in urine, the glucuronides are cleaved by enzymatic hydrolysis and then the analytes are isolated and concentrated using solid phase extraction (SPE). Two sorbent materials are used for sample preparation via SPE, first a material based on molecularly imprinted polymers and then a mixed‐mode cation exchange polymer. Analysis is performed by LC‐MS/MS. Deuterated internal standards are used for calibration. Calibration standards are prepared in pooled urine obtained from non‐smokers and are processed in the same way as the samples to be analysed.
    Keywords 4-(methylnitrosamino)-1-(3 pyridyl)-1-butanol ; 4-(methylnitrosamino)-1-(3 pyridyl)-1-butanone ; Analyses in Biological Materials ; Biomonitoring-Methoden ; Biomonitoring Methods ; LC-MS/MS ; N-nitrosoanabasine ; N-nitrosoanatabine ; N-nitrosonornicotine ; NNAL ; NNK ; NNN ; NAB ; NAT ; TSNA ; biomonitoring ; liquid chromatography-tandem mass spectrometry ; tobacco-specific nitrosamines ; urine
    Language English
    Document type Article
    DOI 10.4126/FRL01-006455558
    Database Repository for Life Sciences

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  2. Article: Tabakspezifische Nitrosamine – Bestimmung von N‐Nitrosoanabasin, N‐Nitrosoanatabin, N‐Nitrosonornikotin und 4‐(Methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanol in Urin mittels LC‐MS/MS : Biomonitoring methods in German language, 2019

    Deutsche Forschungsgemeinschaft. Ständige Senatskommission zur Prüfung Gesundheitsschädlicher Arbeitsstoffe / Scherer, Gerhard / Gilch, Gerhard / Köhler, Dominique / Völkel, Wolfgang / Göen, Thomas / Hartwig, Andrea / MAK Commission

    The MAK collection for occupational health and safety, 4(4):2442-2468

    2019  

    Abstract: The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. This analytical method ... ...

    Institution Deutsche Forschungsgemeinschaft. Ständige Senatskommission zur Prüfung Gesundheitsschädlicher Arbeitsstoffe
    Abstract The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. This analytical method permits the determination of tobacco‐specific nitrosamines (TSNA) in urine using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). The parameters in question are N‐nitrosoanabasine (NAB), N‐nitrosoanatabine (NAT), N‐nitrosonornicotine (NNN) and 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanol (NNAL). NNAL is a metabolite of 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone (NNK). Due to its sensitivity, this method is suitable for the detection of the aforementioned analytes in the urine of smokers. NNAL can also be quantified in the urine of passive smokers. The analytes NAB, NAT, NNN and NNAL are present in urine in both free and glucuronidated forms. For the determination of the total TSNA level in urine, the glucuronides are cleaved by enzymatic hydrolysis and then the analytes are isolated and concentrated using solid phase extraction (SPE). Two sorbent materials are used for sample preparation via SPE, first a material based on molecularly imprinted polymers and then a mixed‐mode cation exchange polymer. Analysis is performed by LC‐MS/MS. Deuterated internal standards are used for calibration. Calibration standards are prepared in pooled urine obtained from non‐smokers and are processed in the same way as the samples to be analysed.
    Keywords 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol ; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanon ; Analysen in biologischem Material ; Biomonitoring ; Biomonitoring-Methoden ; Biomonitoring Methods ; Hochleistungsflüssigchromatographie-Tandemmassenspektrometrie ; LC-MS/MS ; N-Nitrosoanabasin ; N-Nitrosoanatabin ; N-Nitrosonornikotin ; NNAL ; NNK ; NNN ; NAB ; NAT ; TSNA ; Urin ; tabakspezifische Nitrosamine
    Language German
    Document type Article
    DOI 10.4126/FRL01-006455556
    Database Repository for Life Sciences

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  3. Article ; Online: Enrichment and properties of urinary pre-S-phenylmercapturic acid (pre-SPMA).

    Sterz, Katharina / Köhler, Dominique / Schettgen, Thomas / Scherer, Gerhard

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2010  Volume 878, Issue 27, Page(s) 2502–2505

    Abstract: In benzene metabolism, pre-S-phenylmercapturic acid (pre-SPMA) is the precursor to S-phenylmercapturic acid (SPMA). Urinary pre-SPMA/SPMA ratios are variable. For the determination of urinary SPMA as a biomarker of exposure to benzene it is essential to ... ...

    Abstract In benzene metabolism, pre-S-phenylmercapturic acid (pre-SPMA) is the precursor to S-phenylmercapturic acid (SPMA). Urinary pre-SPMA/SPMA ratios are variable. For the determination of urinary SPMA as a biomarker of exposure to benzene it is essential to completely convert pre-SPMA to SPMA. We developed a procedure for the enrichment and determination of urinary pre-SPMA by LC-MS/MS which allowed us to trace the conversion of pre-SPMA to SPMA. Complete conversion was found upon treatment of urine with HCl (37%) at pH 1.1. Previously reported treatment of urine with concentrated H(2)SO(4) was found to yield SPMA levels higher than after HCl treatment. The origin of that extra SPMA amount is unknown. In conclusion, our findings suggest that pre-treatment of urine with HCl to adjust the pH to 0.5-1 is essential for complete conversion of pre-SPMA to SPMA and should be applied prior to analysis of SPMA in urine.
    MeSH term(s) Acetylcysteine/analogs & derivatives ; Acetylcysteine/chemistry ; Acetylcysteine/urine ; Biomarkers/chemistry ; Biomarkers/urine ; Chromatography, Liquid/methods ; Hydrogen-Ion Concentration ; Tandem Mass Spectrometry/methods
    Chemical Substances Biomarkers ; S-phenyl-N-acetylcysteine (4775-80-8) ; Acetylcysteine (WYQ7N0BPYC)
    Language English
    Publishing date 2010-10-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2009.08.043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Bedeutung regionaler Gesundheitspolitik für die Verteilung von Verbandsaufgaben zwischen dem Bundesverband und einzelnen Landesverbänden der Betriebskrankenkassen

    Hodek, Jan-Marc / Greiner, Wolfgang / Köhler, Dominique

    Sozialer Fortschritt : unabhängige Zeitschrift für Sozialpolitik Vol. 56, No. 1 , p. 17-24

    2007  Volume 56, Issue 1, Page(s) 17–24

    Author's details Jan-Marc Hodek, Wolfgang Greiner und Dominique Köhler
    Keywords Gesetzliche Krankenversicherung ; Interessenvertretung ; Föderalismus ; Deutschland
    Language German
    Size graph. Darst.
    Publisher Duncker & Humblot
    Publishing place Berlin
    Document type Article
    Note Zsfassung in engl. Sprache
    ZDB-ID 2088-6
    Database ECONomics Information System

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