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  1. Artikel ; Online: Performance Optimization of a Microfluidic Virus Detection Cartridge: A Numerical and Experimental Study.

    Şenel, Enes Burak / Kizilelma, Bilal / Tamdoğan, Enes / Yorulmaz, Mustafa

    Journal of biomechanical engineering

    2023  Band 145, Heft 10

    Abstract: Detection and imaging of viruses in a complex solution is particularly significant for virology and requires a comprehensive understanding of biosensors. While lab-on-a-chip systems are used in virus detection as biosensors, analysis and optimization of ... ...

    Abstract Detection and imaging of viruses in a complex solution is particularly significant for virology and requires a comprehensive understanding of biosensors. While lab-on-a-chip systems are used in virus detection as biosensors, analysis and optimization of these systems are especially challenging due to the size of the system to be used in the certain application. The system of interest for virus detection is required to be cost efficient and is also needed to be able to easily operable with a simple setup. Moreover, the detailed analysis of these microfluidic systems should be made with precision in order to predict the capabilities and the efficiency of the system accurately. This paper reports on the use of a common commercial computational fluid dynamics (cfd) software for the analysis of a microfluidic lab-on-a-chip virus detection cartridge. This study evaluates the problems commonly encountered during microfluidic applications of cfd softwares particularly in the area of reaction modeling of the antigen-antibody interaction. cfd analysis is later validated and combined with experiments to optimize the amount of dilute solution used in the tests. Thereafter, the geometry of the microchannel is also optimized and optimal test conditions are set for a cost efficient and effective virus detection kit using light microscopy.
    Mesh-Begriff(e) Microfluidics ; Biosensing Techniques ; Microfluidic Analytical Techniques/methods ; Lab-On-A-Chip Devices
    Sprache Englisch
    Erscheinungsdatum 2023-06-29
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 243094-0
    ISSN 1528-8951 ; 0148-0731
    ISSN (online) 1528-8951
    ISSN 0148-0731
    DOI 10.1115/1.4062850
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Ultrasensitive electrochemical immunosensor system for determination of autologous SOX2 antibody.

    Özçelikay-Akyıldız, Göksu / Ünal, Mehmet Altay / Atakan, Şükrü / Gülden, Seçil / Kızılelma, Bilal / Aydın, Safa / Ozkan, Sibel A

    Journal of pharmaceutical and biomedical analysis

    2024  Band 241, Seite(n) 115992

    Abstract: Lung cancer is mainly seen as the cancer type in the world. Lung cancer causes the death of many people. It is classified as large-cell neuroendocrine carcinoma (LCNEC), small-cell lung cancer (SCLC), and adenocarcinoma by the World Health Organization ( ... ...

    Abstract Lung cancer is mainly seen as the cancer type in the world. Lung cancer causes the death of many people. It is classified as large-cell neuroendocrine carcinoma (LCNEC), small-cell lung cancer (SCLC), and adenocarcinoma by the World Health Organization (WHO) in 2015. Small cell lung cancer (SCLC) is a highly aggressive type of cancer, accounting for approximately 20% of all cases. By performing the serological analysis of expression cDNA libraries (SEREX), the humoral immune response of SCLC patients is determined. SEREX of SCLC cell lines using pooled sera of SCLC patients led to the isolation of SOX2 genes. The between SOX2 antigen expression intensity and autologous antibody presence has a significant correlation because SOX2 is the main antigen eliciting anti-SOX responses. Electrochemical biosensors take much attention because of their simplicity, selectivity, and sensitivity in clinical analysis. Antibody-based surface recognizes antibody-specific antigens. This work aims to fabricate an immunosensor for determining autologous SOX2 antibodies using a multi-walled carbon nanotube-modified screen-printed electrode (DRP-MWCNT). All immobilization processes were evaluated with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The critical parameters were optimized, such as EDC/NHS concentration and time, SOX2 protein concentration and incubation time, BSA ratio, BSA blocking time, and anti-SOX2 antibody incubation time. The developed immunosensor, under optimal conditions, shows a linear response of autologous SOX2 antibody between 0.005 ng.mL
    Mesh-Begriff(e) Humans ; Immunoassay/methods ; Biosensing Techniques/methods ; Antibodies ; Enzyme-Linked Immunosorbent Assay ; Lung Neoplasms ; Small Cell Lung Carcinoma ; Electrochemical Techniques ; Electrodes ; Limit of Detection ; Gold ; SOXB1 Transcription Factors
    Chemische Substanzen Antibodies ; Gold (7440-57-5) ; SOX2 protein, human ; SOXB1 Transcription Factors
    Sprache Englisch
    Erscheinungsdatum 2024-01-20
    Erscheinungsland England
    Dokumenttyp Journal Article ; Review
    ZDB-ID 604917-5
    ISSN 1873-264X ; 0731-7085
    ISSN (online) 1873-264X
    ISSN 0731-7085
    DOI 10.1016/j.jpba.2024.115992
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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