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  1. Artikel ; Online: Hydrogen Peroxide Production of Individual Nanosecond Pulsed Discharges Submerged in Water of Elevated Conductivity.

    Rataj, Raphael / Werneburg, Matthias / Below, Harald / Kolb, Juergen F

    Chemphyschem : a European journal of chemical physics and physical chemistry

    2023  Band 24, Heft 19, Seite(n) e202300143

    Abstract: The production of hydrogen peroxide ( ... ...

    Abstract The production of hydrogen peroxide (H
    Sprache Englisch
    Erscheinungsdatum 2023-08-10
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 2025223-7
    ISSN 1439-7641 ; 1439-4235
    ISSN (online) 1439-7641
    ISSN 1439-4235
    DOI 10.1002/cphc.202300143
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  2. Artikel: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields.

    Wolff, Christina M / Kolb, Juergen F / Bekeschus, Sander

    Biomedicines

    2022  Band 10, Heft 12

    Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

    Abstract In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.
    Sprache Englisch
    Erscheinungsdatum 2022-11-30
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines10123084
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Analysis of microstructural parameters of trabecular bone based on electrical impedance spectroscopy and deep neural networks.

    Wei, Wenzuo / Shi, Fukun / Kolb, Juergen F

    Bioelectrochemistry (Amsterdam, Netherlands)

    2022  Band 148, Seite(n) 108232

    Abstract: The potential of electrical impedance spectroscopy (EIS) was demonstrated for the investigation of microstructural properties of osseous tissue. Therefore, a deep neural network (DNN) was implemented for a sensitive assessment of different structural ... ...

    Abstract The potential of electrical impedance spectroscopy (EIS) was demonstrated for the investigation of microstructural properties of osseous tissue. Therefore, a deep neural network (DNN) was implemented for a sensitive assessment of different structural features that were derived on the basis of dielectric parameters, especially relative permittivities, recorded over a frequency range from 40 Hz to 5 MHz. The advantages of the developed method over conventional approaches, including equivalent circuit models (ECMs), linear regression and effective medium approximation (EMA), is the comprehensive quantification of bone morphologies by several microstructural parameters simultaneously, such as bone volume fraction (BV/TV), bone surface-volume-ratio (BS/BV), structure model index (SMI), trabecular number (Tb.N) and trabecular thickness (Tb.Th). The comparison of predictions of the DNN with an analysis of µCT-images confirmed a high accuracy for different microstructural parameters, which was indicated by corresponding Pearson correlation coefficients, especially for Tb.Th (r = 0.89) and BS/BV (r = 0.80). Concurrently, the approach was able to unambiguously discriminate anatomically similar bone regions (femoral head, greater trochanter and femoral neck) and therefore was capable to determine the morphological status of osseous tissue in detail. The classification was more discriminative than one based on classical linear discriminant analysis (LDA), due to the distinguishing features extracted by the DNN model. Accordingly, the method and model can serve as a potential tool for evaluating bone quality and bone status.
    Mesh-Begriff(e) Cancellous Bone/diagnostic imaging ; Dielectric Spectroscopy ; Femur/diagnostic imaging ; Neural Networks, Computer
    Sprache Englisch
    Erscheinungsdatum 2022-08-13
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 2010650-6
    ISSN 1878-562X ; 0302-4598 ; 1567-5394
    ISSN (online) 1878-562X
    ISSN 0302-4598 ; 1567-5394
    DOI 10.1016/j.bioelechem.2022.108232
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Enhanced resolution impedimetric analysis of cell responses from the distribution of relaxation times.

    Shi, Fukun / Kolb, Juergen F

    Biosensors & bioelectronics

    2020  Band 157, Seite(n) 112149

    Abstract: A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis ...

    Abstract A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis for the distribution of relaxation times (DRT). The DRT method provides high resolution for the identification of different polarization processes without a priori assumptions, as they are needed by more conventional approaches, such as an evaluation by equivalent circuit models. Accordingly, the physical properties of cells and their changes due to external stimuli can be uncovered and visualized and dispersion mechanisms introduced by Schwan et al. clearly identified. These are in particular relaxation processes at about 100 kHz that are associated with cell membrane characteristics and dominating respective changes of the distribution function for epithelial cell monolayers after exposure. A relatively moderate evolution at about 10 kHz may represent the polarization of extracellular matrices. Relaxation processes at around 1 MHz were suggested to be associated with intracellular changes. Conversely, the distribution of relaxation times can aid the optimization of the experimental design with respect to intended responses by an external stimulus.
    Mesh-Begriff(e) Algorithms ; Animals ; Biosensing Techniques/methods ; Cell Line ; Cell Membrane/chemistry ; Dielectric Spectroscopy/methods ; Electricity ; Epithelial Cells/chemistry ; Epithelial Cells/cytology ; Liver/chemistry ; Liver/cytology ; Normal Distribution ; Rats
    Sprache Englisch
    Erscheinungsdatum 2020-03-30
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2020.112149
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  5. Artikel: Enhanced resolution impedimetric analysis of cell responses from the distribution of relaxation times

    Shi, Fukun / Kolb, Juergen F

    Biosensors & bioelectronics. 2020 June 01, v. 157

    2020  

    Abstract: A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis ...

    Abstract A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis for the distribution of relaxation times (DRT). The DRT method provides high resolution for the identification of different polarization processes without a priori assumptions, as they are needed by more conventional approaches, such as an evaluation by equivalent circuit models. Accordingly, the physical properties of cells and their changes due to external stimuli can be uncovered and visualized and dispersion mechanisms introduced by Schwan et al. clearly identified. These are in particular relaxation processes at about 100 kHz that are associated with cell membrane characteristics and dominating respective changes of the distribution function for epithelial cell monolayers after exposure. A relatively moderate evolution at about 10 kHz may represent the polarization of extracellular matrices. Relaxation processes at around 1 MHz were suggested to be associated with intracellular changes. Conversely, the distribution of relaxation times can aid the optimization of the experimental design with respect to intended responses by an external stimulus.
    Schlagwörter biosensors ; cell membranes ; dielectric spectroscopy ; epithelial cells ; experimental design ; models ; physical properties ; pulsed electric fields
    Sprache Englisch
    Erscheinungsverlauf 2020-0601
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2020.112149
    Datenquelle NAL Katalog (AGRICOLA)

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 2275: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  6. Artikel ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

    Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

    Biomedicines : open access journal

    2022  Band 10, Heft , Nr. 12

    Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

    Abstract In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.

    publishedVersion
    Schlagwörter apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Verlag Basel : MDPI
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
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  7. Artikel ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

    Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

    Biomedicines : open access journal

    2022  Band 10, Heft , Nr. 12

    Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

    Abstract In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.

    publishedVersion
    Schlagwörter apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Verlag Basel : MDPI
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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  8. Artikel ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

    Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

    Biomedicines : open access journal

    2022  Band 10, Heft , Nr. 12

    Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

    Abstract In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.

    publishedVersion
    Schlagwörter apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Verlag Basel : MDPI
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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  9. Artikel ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

    Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

    Biomedicines : open access journal

    2022  Band 10, Heft , Nr. 12

    Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

    Abstract In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.

    publishedVersion
    Schlagwörter apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Verlag Basel : MDPI
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  10. Artikel ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

    Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

    Biomedicines : open access journal

    2022  Band 10, Heft , Nr. 12

    Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

    Abstract In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.

    publishedVersion
    Schlagwörter apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Verlag Basel : MDPI
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

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