Artikel ; Online: Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef.
2024 Band 15, Heft 1, Seite(n) 3813
Abstract: Innate antiviral factors are essential for effective defense against viral pathogens. However, the identity of major restriction mechanisms remains elusive. Current approaches to discover antiviral factors usually focus on the initial steps of viral ... ...
Abstract | Innate antiviral factors are essential for effective defense against viral pathogens. However, the identity of major restriction mechanisms remains elusive. Current approaches to discover antiviral factors usually focus on the initial steps of viral replication and are limited to a single round of infection. Here, we engineered libraries of >1500 replication-competent HIV-1 constructs each expressing a single gRNAs to target >500 cellular genes for virus-driven discovery of antiviral factors. Passaging in CD4 |
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Mesh-Begriff(e) | Humans ; HIV-1/genetics ; HIV-1/physiology ; Virus Replication/genetics ; nef Gene Products, Human Immunodeficiency Virus/genetics ; nef Gene Products, Human Immunodeficiency Virus/metabolism ; CD4-Positive T-Lymphocytes/virology ; CD4-Positive T-Lymphocytes/metabolism ; CD4-Positive T-Lymphocytes/immunology ; HEK293 Cells ; CRISPR-Cas Systems ; HIV Infections/virology ; HIV Infections/genetics ; HIV Infections/immunology ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Phosphoproteins/metabolism ; Phosphoproteins/genetics ; rhoA GTP-Binding Protein/metabolism ; rhoA GTP-Binding Protein/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Virus Internalization |
Chemische Substanzen | nef Gene Products, Human Immunodeficiency Virus ; RNA, Guide, CRISPR-Cas Systems ; Phosphoproteins ; rhoA GTP-Binding Protein (EC 3.6.5.2) ; nef protein, Human immunodeficiency virus 1 |
Sprache | Englisch |
Erscheinungsdatum | 2024-05-07 |
Erscheinungsland | England |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural |
ZDB-ID | 2553671-0 |
ISSN | 2041-1723 ; 2041-1723 |
ISSN (online) | 2041-1723 |
ISSN | 2041-1723 |
DOI | 10.1038/s41467-024-48228-x |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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